D0CD53 (D0CD53_ACIBA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Malate dehydrogenase HAMAP MF_01517
EC=1.1.1.37 HAMAP MF_01517

Gene names

Name:	mdh HAMAP MF_01517
ORF Names:	HMPREF0010_02683 EMBL EEX02990.1

Organism

Acinetobacter baumannii ATCC 19606 EMBL EEX02990.1

Taxonomic identifier

575584 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter › Acinetobacter calcoaceticus/baumannii complex

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP MF_01517
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP MF_01517 SAAS SAAS010945
Subunit structure	Homodimer By similarity. HAMAP MF_01517 SAAS SAAS010945
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 2 family. HAMAP MF_01517

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle HAMAP MF_01517 SAAS SAAS010945
Ligand	NAD HAMAP MF_01517 SAAS SAAS010945
Molecular function	Oxidoreductase HAMAP MF_01517
Gene Ontology (GO)
Biological process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	11 – 17	7	NAD By similarity HAMAP MF_01517
	Nucleotide binding	131 – 133	3	NAD By similarity HAMAP MF_01517
Sites
	Active site	189	1	Proton acceptor By similarity HAMAP MF_01517
	Binding site	94	1	Substrate By similarity HAMAP MF_01517
	Binding site	100	1	Substrate By similarity HAMAP MF_01517
	Binding site	107	1	NAD By similarity HAMAP MF_01517
	Binding site	114	1	NAD By similarity HAMAP MF_01517
	Binding site	133	1	Substrate By similarity HAMAP MF_01517
	Binding site	164	1	Substrate By similarity HAMAP MF_01517

Sequences

	Sequence		Length	Mass (Da)
	D0CD53 [UniParc]. Last modified November 24, 2009. Version 1. Checksum: 4A3ABDD04F615F6E	FASTA	328	35,323
	10 20 30 40 50 60 MKQPVRVAVT GAAGQIGYSL LFRIASGEML GKDQPVILQL LEVPVEKAQQ ALKGVMMELD 70 80 90 100 110 120 DCAFPLLAGM IGTDDPKVAF KDADYALLVG SRPRGPGMER ADLLKVNGEI FIGQGQALNE 130 140 150 160 170 180 VASRDVKVLV VGNPANTNAY IAMKSAPDLP AKNFTAMLRL DHNRALTQVA QKAGVAVADI 190 200 210 220 230 240 EKLTVWGNHS PTMYADYRFA TANGESLKDK INDPAWNKDV FLPTVGKRGA AIIEARGLSS 250 260 270 280 290 300 AASAANAAID HMRDWALGTN GKWVTMGVPS DGSYGIPEGV MFGFPVTTEN GEYKIVQGLE 310 320 IDEFSRERIN FTLNELEEER AAIADMVK

References

[1]

"The Genome Sequence of Acinetobacter baumannii strain ATCC 19606." The Broad Institute Genome Sequencing Platform Ward D., Feldgarden M., Earl A., Young S.K., Zeng Q., Koehrsen M., Alvarado L., Berlin A., Bochicchio J., Borenstein D., Chapman S., Chen Z., Engels R., Freedman E., Gellesch M., Goldberg J., Griggs A., Gujja S. , Heilman E., Heiman D., Hepburn T., Howarth C., Jen D., Larson L., Lewis B., Mehta T., Park D., Pearson M., Roberts A., Saif S., Shea T., Shenoy N., Sisk P., Stolte C., Sykes S., Thomson T., Walk T., White J., Yandava C., Peleg A., Haas B., Nusbaum C., Birren B. Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 19606 EMBL EEX02990.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	GG704576 Genomic DNA. Translation: EEX02990.1.
3D structure databases
ProteinModelPortal	D0CD53.
SMR	D0CD53. Positions 2-327.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
PATRIC	24334905. VBIAciBau3967_2623.
Family and domain databases
HAMAP	MF_01517. Malate_dehydrog_2. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR010945. Malate_DH_type2. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR23382. MDH_SF1. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01759. MalateDH-SF1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

D0CD53_ACIBA

Accession

Primary (citable) accession number: D0CD53

Entry history

Integrated into UniProtKB/TrEMBL:	November 24, 2009
Last sequence update:	November 24, 2009
Last modified:	December 14, 2011
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)