Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

D0C9L6

- D0C9L6_ACIBA

UniProt

D0C9L6 - D0C9L6_ACIBA

Protein

Peptidyl-tRNA hydrolase

Gene

pth

Organism
Acinetobacter baumannii ATCC 19606 = CIP 70.34
Status
Unreviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 31 (01 Oct 2014)
      Sequence version 1 (24 Nov 2009)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.UniRule annotation

    Catalytic activityi

    N-substituted aminoacyl-tRNA + H2O = N-substituted amino acid + tRNA.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei22 – 221PhosphateImported
    Binding sitei116 – 1161PhosphateImported

    GO - Molecular functioni

    1. aminoacyl-tRNA hydrolase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    HydrolaseUniRule annotationImported

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peptidyl-tRNA hydrolaseUniRule annotation (EC:3.1.1.29UniRule annotation)
    Short name:
    PTHUniRule annotation
    Gene namesi
    Name:pthUniRule annotationImported
    ORF Names:F911_03144Imported, HMPREF0010_01329Imported
    OrganismiAcinetobacter baumannii ATCC 19606 = CIP 70.34Imported
    Taxonomic identifieri575584 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacterAcinetobacter calcoaceticus/baumannii complex

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3WH4X-ray1.36A1-193[»]
    4FOPX-ray1.86A1-193[»]
    4FOTX-ray2.20A1-193[»]
    4HOYX-ray1.78A1-193[»]
    4IKOX-ray1.90A1-193[»]
    4JWKX-ray1.87A1-193[»]
    4JX9X-ray1.40A1-193[»]
    4JY7X-ray1.90A1-193[»]
    4LWQX-ray1.38A1-193[»]
    4LWRX-ray1.10A1-193[»]
    4OLJX-ray1.49A1-193[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PTH family.UniRule annotation

    Family and domain databases

    Gene3Di3.40.50.1470. 1 hit.
    HAMAPiMF_00083. Pept_tRNA_hydro_bact.
    InterProiIPR001328. Pept_tRNA_hydro.
    IPR018171. Pept_tRNA_hydro_CS.
    [Graphical view]
    PANTHERiPTHR17224. PTHR17224. 1 hit.
    PfamiPF01195. Pept_tRNA_hydro. 1 hit.
    [Graphical view]
    SUPFAMiSSF53178. SSF53178. 1 hit.
    TIGRFAMsiTIGR00447. pth. 1 hit.
    PROSITEiPS01195. PEPT_TRNA_HYDROL_1. 1 hit.
    PS01196. PEPT_TRNA_HYDROL_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    D0C9L6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSNISLIVGL GNPGSEYAQT RHNAGFWFVE QLADKYGITL KNDPKFHGIS    50
    GRGNIEGHDV RLLLPMTYMN RSGQSVVPFS KFYQIAPEAI LIAHDELDMN 100
    PGVIRLKTGG GHGGHNGLRD IVPHIGPNFH RLRIGIGHPG SKERVSGHVL 150
    GKAPSNEQSL MDGAIDHALS KVKLLVQGQV PQAMNQINAY KPA 193
    Length:193
    Mass (Da):20,938
    Last modified:November 24, 2009 - v1
    Checksum:i08025EF679FC28FF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    GG704573 Genomic DNA. Translation: EEX03935.1.
    APRG01000016 Genomic DNA. Translation: ENW73725.1.

    Genome annotation databases

    EnsemblBacteriaiEEX03935; EEX03935; HMPREF0010_01329.
    ENW73725; ENW73725; F911_03144.
    PATRICi24332185. VBIAciBau3967_1302.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    GG704573 Genomic DNA. Translation: EEX03935.1 .
    APRG01000016 Genomic DNA. Translation: ENW73725.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3WH4 X-ray 1.36 A 1-193 [» ]
    4FOP X-ray 1.86 A 1-193 [» ]
    4FOT X-ray 2.20 A 1-193 [» ]
    4HOY X-ray 1.78 A 1-193 [» ]
    4IKO X-ray 1.90 A 1-193 [» ]
    4JWK X-ray 1.87 A 1-193 [» ]
    4JX9 X-ray 1.40 A 1-193 [» ]
    4JY7 X-ray 1.90 A 1-193 [» ]
    4LWQ X-ray 1.38 A 1-193 [» ]
    4LWR X-ray 1.10 A 1-193 [» ]
    4OLJ X-ray 1.49 A 1-193 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai EEX03935 ; EEX03935 ; HMPREF0010_01329 .
    ENW73725 ; ENW73725 ; F911_03144 .
    PATRICi 24332185. VBIAciBau3967_1302.

    Family and domain databases

    Gene3Di 3.40.50.1470. 1 hit.
    HAMAPi MF_00083. Pept_tRNA_hydro_bact.
    InterProi IPR001328. Pept_tRNA_hydro.
    IPR018171. Pept_tRNA_hydro_CS.
    [Graphical view ]
    PANTHERi PTHR17224. PTHR17224. 1 hit.
    Pfami PF01195. Pept_tRNA_hydro. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53178. SSF53178. 1 hit.
    TIGRFAMsi TIGR00447. pth. 1 hit.
    PROSITEi PS01195. PEPT_TRNA_HYDROL_1. 1 hit.
    PS01196. PEPT_TRNA_HYDROL_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE.
      Strain: ATCC 19606Imported.
    2. "The mode of inhibitor binding to peptidyl-tRNA hydrolase: binding studies and structure determination of unbound and bound peptidyl-tRNA hydrolase from Acinetobacter baumannii."
      Kaushik S., Singh N., Yamini S., Singh A., Sinha M., Arora A., Kaur P., Sharma S., Singh T.P.
      PLoS ONE 8:e67547-e67547(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS).
    3. Cited for: NUCLEOTIDE SEQUENCE.
      Strain: CIP 70.34Imported.
    4. "Crystal structure of native peptidyl t-RNA hydrolase from Acinetobacter baumannii at 1.38A resolution."
      Kaushik S., Singh N., Sinha M., Kaur P., Sharma S., Singh T.P.
      Submitted (JUL-2013) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.38 ANGSTROMS).
    5. "Crystal structure of the ternary complex of peptidyl tRNA hydrolase from Acinetobacter baumannii with cytosine arabinoside and phosphate ion at 1.1A resolution."
      Kaushik S., Singh N., Sinha M., Kaur P., Sharma S., Singh T.P.
      Submitted (JUL-2013) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.10 ANGSTROMS).
    6. "Crystal structure of peptidyl t-RNA hydrolase from Acinetobacter baumannii in unbound state at 1.36A resolution."
      Kaushik S., Singh N., Sinha M., Kaur P., Sharma S., Singh T.P.
      Submitted (AUG-2013) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.36 ANGSTROMS).
    7. "Crystal structure of Arg119Gln mutant of Peptidyl-tRNA hydrolase from Acinetobacter Baumannii at 1.49 A resolution."
      Sikarwar J., Dube D., Kaushik S., Sinha M., Kaur P., Sharma S., Singh T.P.
      Submitted (JAN-2014) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.49 ANGSTROMS).

    Entry informationi

    Entry nameiD0C9L6_ACIBA
    AccessioniPrimary (citable) accession number: D0C9L6
    Entry historyi
    Integrated into UniProtKB/TrEMBL: November 24, 2009
    Last sequence update: November 24, 2009
    Last modified: October 1, 2014
    This is version 31 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3