D0BAW2 (D0BAW2_BRUSS) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00818
EC=1.7.1.13 HAMAP MF_00818

Alternative name(s):
7-cyano-7-carbaguanine reductase HAMAP MF_00818
NADPH-dependent nitrile oxidoreductase HAMAP MF_00818
PreQ(0) reductase HAMAP MF_00818

Gene names

Name:	queF HAMAP MF_00818
ORF Names:	BAVG_0403 EMBL EEW90884.1

Organism

Brucella suis bv. 4 str. 40 EMBL EEW90884.1

Taxonomic identifier

520488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	155 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP MF_00818 SAAS SAAS016856
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00818 SAAS SAAS016856
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00818 SAAS SAAS016856
Subcellular location	Cytoplasm By similarity HAMAP MF_00818 SAAS SAAS016856.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily. HAMAP MF_00818

Ontologies

Keywords
Biological process	Queuosine biosynthesis HAMAP MF_00818 SAAS SAAS016856
Cellular component	Cytoplasm HAMAP MF_00818 SAAS SAAS016856
Ligand	NADP HAMAP MF_00818 SAAS SAAS016856
Molecular function	Oxidoreductase HAMAP MF_00818 SAAS SAAS016856
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP preQ1 synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	D0BAW2 [UniParc]. Last modified November 24, 2009. Version 1. Checksum: EB93CD3CC760ADE1	FASTA	155	17,416
	10 20 30 40 50 60 MSENTIYSGL KQLGSHTDIP LTPEEAVLER VANPQEGTPY CVRFTAPEFT SLCPMTGQPD 70 80 90 100 110 120 FAHLVIDYVP GKWLVESKSL KLFLFSFRNH GAFHEDCTVT IGKRLVDLLE PEWLRIGGYW 130 140 150 YPRGGIPIDV FYQTGAAPLN VWIPEQGVAN YRGRG

References

[1]

"The Genome Sequence of Brucella suis bv. 4 str. 40." The Broad Institute Genome Sequencing Platform Ward D., Young S.K., Kodira C.D., Zeng Q., Koehrsen M., Alvarado L., Berlin A., Borenstein D., Chen Z., Engels R., Freedman E., Gellesch M., Goldberg J., Griggs A., Gujja S., Heiman D., Hepburn T., Howarth C. , Jen D., Larson L., Lewis B., Mehta T., Park D., Pearson M., Roberts A., Saif S., Shea T., Shenoy N., Sisk P., Stolte C., Sykes S., Walk T., White J., Yandava C., Whatmore A.M., Perrett L.L., O'Callaghan D., Nusbaum C., Galagan J., Birren B. Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: 40 EMBL EEW90884.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	GG703793 Genomic DNA. Translation: EEW90884.1.
3D structure databases
ProteinModelPortal	D0BAW2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
PATRIC	24291227. VBIBruSui38587_1722.
Phylogenomic databases
OMA	HEDCTVA.
Family and domain databases
HAMAP	MF_00818. QueF_type1. [Tree]
InterPro	IPR016856. CN_OxRdtase_NADPH-dep_QueF. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF027377. Nitrile_oxidored_QueF. 1 hit.
TIGRFAMs	TIGR03139. QueF-II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

D0BAW2_BRUSS

Accession

Primary (citable) accession number: D0BAW2

Entry history

Integrated into UniProtKB/TrEMBL:	November 24, 2009
Last sequence update:	November 24, 2009
Last modified:	December 14, 2011
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)