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D0B3F7 (D0B3F7_BRUME) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 14. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
3-isopropylmalate dehydratase large subunit 2 HAMAP MF_01026
EC=4.2.1.33 HAMAP MF_01026
Alternative name(s):
Alpha-IPM isomerase 2 HAMAP MF_01026
Isopropylmalate isomerase 2 HAMAP MF_01026
Gene names
Name:leuC2 HAMAP MF_01026
ORF Names:BAWG_0039 EMBL EEW87931.1
OrganismBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifier224914 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length469 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate By similarity. HAMAP MF_01026 SAAS SAAS004430

Catalytic activity

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmaleate + H2O. HAMAP MF_01026 SAAS SAAS004430

(2S)-2-isopropylmaleate + H2O = (2S)-2-isopropylmalate. HAMAP MF_01026 SAAS SAAS004430

Cofactor

Binds 1 4Fe-4S cluster per subunit By similarity. HAMAP MF_01026 SAAS SAAS004430

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4. HAMAP MF_01026 SAAS SAAS004430

Subunit structure

Heterodimer of LeuC and LeuD By similarity. HAMAP MF_01026 SAAS SAAS004430

Sequence similarities

Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily. HAMAP MF_01026

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding3501Iron-sulfur (4Fe-4S) By similarity HAMAP MF_01026
Metal binding4101Iron-sulfur (4Fe-4S) By similarity HAMAP MF_01026
Metal binding4131Iron-sulfur (4Fe-4S) By similarity HAMAP MF_01026

Sequences

Sequence LengthMass (Da)Tools
D0B3F7 [UniParc].

Last modified November 24, 2009. Version 1.
Checksum: 14442D949C40BCDF

FASTA46950,692
        10         20         30         40         50         60 
MSAPRTLYDK IWDDHVVDQQ EDGTCLLYID RHLVHEVTSP QAFEGLHMAG RPVRHPEKTL 

        70         80         90        100        110        120 
AVVDHNVPTS PDRINGIQNE ESRIQVEALA RNAADFGVEY YSERDKRQGI VHIVGPEQGF 

       130        140        150        160        170        180 
TLPGMTIVCG DSHTSTHGAF GALAHGIGTS EVEHVLATQT LIQKKAKNML VRVDGKLPAG 

       190        200        210        220        230        240 
VTAKDIVLAI IGEIGTAGGT GYVIEYAGEA IRSLSMEGRM TICNMSIEGG ARAGLIAPDE 

       250        260        270        280        290        300 
TTFEYIKGRP RAPQGETLEQ AINYWKTLHS DEGAHFDKIV TLDAGSLPPI VSWGSSPEDV 

       310        320        330        340        350        360 
VSVTGVVPNP DDIADETKRA SKWRALDYMG LKPGTKITDI AVDRVFIGSC TNGRIEDLRA 

       370        380        390        400        410        420 
AAKVVEGKKV APTVNAMIVP GSGLVKEQAE AEGLHKIFIE AGFDWREPGC SMCLAMNDDR 

       430        440        450        460 
LKPGERCAST SNRNFEGRQG FKGRTHLVSP AMAAAAAIAG HFVDIRAWK

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		GG703778 Genomic DNA. Translation: EEW87931.1.
RefSeqNP_539075.1. NC_003317.1.

3D structure databases

ProteinModelPortalD0B3F7.
ModBaseSearch...

Protein-protein interaction databases

STRINGD0B3F7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1195869.
PATRIC17795361. VBIBruMel146950_0847.

Phylogenomic databases

OMADIRQGIV.

Family and domain databases

HAMAPMF_01026. LeuC_type1.
[Tree]
InterProIPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR015936. Homoacnase/3IPM_deHydtase_su.
[Graphical view]
Gene3DG3DSA:3.30.499.10. Acnase/IPM_dHydase_lsu_aba_1/3. 3 hits.
G3DSA:3.40.1060.10. Aconitase/IPMdHydase_lsu_aba_2. 1 hit.
PANTHERPTHR11670. Aconitase-like_core. 1 hit.
PTHR11670:SF6. Homoacnase/3IPM_dehydase_s/lsu. 1 hit.
PfamPF00330. Aconitase. 1 hit.
[Graphical view]
PRINTSPR00415. ACONITASE.
SUPFAMSSF53732. Aconitase_N. 1 hit.
TIGRFAMsTIGR00170. LeuC. 1 hit.
PROSITEPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD0B3F7_BRUME
AccessionPrimary (citable) accession number: D0B3F7
Entry history
Integrated into UniProtKB/TrEMBL: November 24, 2009
Last sequence update: November 24, 2009
Last modified: January 25, 2012
This is version 14 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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