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Protein

NADH-quinone oxidoreductase subunit B

Gene

nuoB

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.UniRule annotation

Catalytic activityi

NADH + quinone = NAD+ + quinol.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi79 – 791Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi80 – 801Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi144 – 1441Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi174 – 1741Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. iron ion binding Source: UniProtKB-HAMAP
  3. NADH dehydrogenase (ubiquinone) activity Source: InterPro
  4. quinone binding Source: UniProtKB-KW

GO - Biological processi

  1. transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotation

Keywords - Biological processi

TransportUniRule annotation

Keywords - Ligandi

4Fe-4SUniRule annotation, Iron, Iron-sulfur, Metal-binding, NADUniRule annotation, UbiquinoneUniRule annotation

Enzyme and pathway databases

BioCyciBMEL224914:GCJ0-1192-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit BUniRule annotation (EC:1.6.99.5UniRule annotation)
Alternative name(s):
NADH dehydrogenase I subunit BUniRule annotation
NDH-1 subunit BUniRule annotation
Gene namesi
Name:nuoBUniRule annotation
ORF Names:BAWG_1052Imported
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)Imported
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Subcellular locationi

Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membraneUniRule annotation, Cell membrane, Membrane

PTM / Processingi

Keywords - PTMi

QuinoneUniRule annotation

Interactioni

Subunit structurei

NDH-1 is composed of 14 different subunits. Subunits NuoB, C, D, E, F, and G constitute the peripheral sector of the complex.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliD0B2M2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 20 kDa subunit family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0377.
KOiK00331.
OMAiHYDNERF.

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
HAMAPiMF_01356. NDH1_NuoB.
InterProiIPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR006138. NADH_UQ_OxRdtase_20Kd_su.
[Graphical view]
PfamiPF01058. Oxidored_q6. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01957. nuoB_fam. 1 hit.
PROSITEiPS01150. COMPLEX1_20K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D0B2M2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEGGAGMGL TGTNTTLVAP QPKGILDPRT GKPVGSDDAF FNDLNGELSD
60 70 80 90 100
KGFIVTSADA LITWARTGSL MWMTFGLACC AVEMMHISMP RYDAERFGIA
110 120 130 140 150
PRASPRQSDV MIVAGTLTNK MAPALRKVYD QMPEPRYVIS MGSCANGGGY
160 170 180 190 200
YHYSYSVVRG CDRVVPVDIY VPGCPPTAEA LLYGILLLQK KIRRTGTIER
Length:200
Mass (Da):21,664
Last modified:November 24, 2009 - v1
Checksum:i65410FA2F51B8DC6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GG703778 Genomic DNA. Translation: EEW88944.1.
RefSeqiNP_540074.1. NC_003317.1.

Genome annotation databases

EnsemblBacteriaiEEW88944; EEW88944; BAWG_1052.
GeneIDi1196868.
KEGGibme:BMEI1157.
PATRICi17797704. VBIBruMel146950_0667.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GG703778 Genomic DNA. Translation: EEW88944.1.
RefSeqiNP_540074.1. NC_003317.1.

3D structure databases

ProteinModelPortaliD0B2M2.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiEEW88944; EEW88944; BAWG_1052.
GeneIDi1196868.
KEGGibme:BMEI1157.
PATRICi17797704. VBIBruMel146950_0667.

Phylogenomic databases

eggNOGiCOG0377.
KOiK00331.
OMAiHYDNERF.

Enzyme and pathway databases

BioCyciBMEL224914:GCJ0-1192-MONOMER.

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
HAMAPiMF_01356. NDH1_NuoB.
InterProiIPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR006138. NADH_UQ_OxRdtase_20Kd_su.
[Graphical view]
PfamiPF01058. Oxidored_q6. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01957. nuoB_fam. 1 hit.
PROSITEiPS01150. COMPLEX1_20K. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 16MImported.

Entry informationi

Entry nameiD0B2M2_BRUME
AccessioniPrimary (citable) accession number: D0B2M2
Entry historyi
Integrated into UniProtKB/TrEMBL: November 24, 2009
Last sequence update: November 24, 2009
Last modified: January 7, 2015
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.