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D0B2M2

- D0B2M2_BRUME

UniProt

D0B2M2 - D0B2M2_BRUME

Protein

NADH-quinone oxidoreductase subunit B

Gene

nuoB

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 32 (01 Oct 2014)
      Sequence version 1 (24 Nov 2009)
      Previous versions | rss
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    Functioni

    NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.UniRule annotation

    Catalytic activityi

    NADH + quinone = NAD+ + quinol.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi79 – 791Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi80 – 801Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi144 – 1441Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi174 – 1741Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. iron ion binding Source: UniProtKB-HAMAP
    3. NADH dehydrogenase (ubiquinone) activity Source: InterPro
    4. quinone binding Source: UniProtKB-KW

    GO - Biological processi

    1. transport Source: UniProtKB-KW

    Keywords - Molecular functioni

    OxidoreductaseUniRule annotation

    Keywords - Biological processi

    TransportUniRule annotation

    Keywords - Ligandi

    4Fe-4SUniRule annotation, Iron, Iron-sulfur, Metal-binding, NADUniRule annotation, UbiquinoneUniRule annotation

    Enzyme and pathway databases

    BioCyciBMEL224914:GCJ0-1192-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH-quinone oxidoreductase subunit BUniRule annotation (EC:1.6.99.5UniRule annotation)
    Alternative name(s):
    NADH dehydrogenase I subunit BUniRule annotation
    NDH-1 subunit BUniRule annotation
    Gene namesi
    Name:nuoBUniRule annotation
    ORF Names:BAWG_1052Imported
    OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)Imported
    Taxonomic identifieri224914 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

    Subcellular locationi

    Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membraneUniRule annotation, Cell membrane, Membrane

    PTM / Processingi

    Keywords - PTMi

    QuinoneUniRule annotation

    Interactioni

    Subunit structurei

    NDH-1 is composed of 14 different subunits. Subunits NuoB, C, D, E, F, and G constitute the peripheral sector of the complex.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliD0B2M2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the complex I 20 kDa subunit family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0377.
    KOiK00331.
    OMAiNNSAEYV.

    Family and domain databases

    Gene3Di3.40.50.700. 1 hit.
    HAMAPiMF_01356. NDH1_NuoB.
    InterProiIPR006137. NADH_UbQ_OxRdtase-like_20kDa.
    IPR006138. NADH_UQ_OxRdtase_20Kd_su.
    [Graphical view]
    PfamiPF01058. Oxidored_q6. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01957. nuoB_fam. 1 hit.
    PROSITEiPS01150. COMPLEX1_20K. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    D0B2M2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEEGGAGMGL TGTNTTLVAP QPKGILDPRT GKPVGSDDAF FNDLNGELSD    50
    KGFIVTSADA LITWARTGSL MWMTFGLACC AVEMMHISMP RYDAERFGIA 100
    PRASPRQSDV MIVAGTLTNK MAPALRKVYD QMPEPRYVIS MGSCANGGGY 150
    YHYSYSVVRG CDRVVPVDIY VPGCPPTAEA LLYGILLLQK KIRRTGTIER 200
    Length:200
    Mass (Da):21,664
    Last modified:November 24, 2009 - v1
    Checksum:i65410FA2F51B8DC6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    GG703778 Genomic DNA. Translation: EEW88944.1.
    RefSeqiNP_540074.1. NC_003317.1.

    Genome annotation databases

    EnsemblBacteriaiEEW88944; EEW88944; BAWG_1052.
    GeneIDi1196868.
    KEGGibme:BMEI1157.
    PATRICi17797704. VBIBruMel146950_0667.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    GG703778 Genomic DNA. Translation: EEW88944.1 .
    RefSeqi NP_540074.1. NC_003317.1.

    3D structure databases

    ProteinModelPortali D0B2M2.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai EEW88944 ; EEW88944 ; BAWG_1052 .
    GeneIDi 1196868.
    KEGGi bme:BMEI1157.
    PATRICi 17797704. VBIBruMel146950_0667.

    Phylogenomic databases

    eggNOGi COG0377.
    KOi K00331.
    OMAi NNSAEYV.

    Enzyme and pathway databases

    BioCyci BMEL224914:GCJ0-1192-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.700. 1 hit.
    HAMAPi MF_01356. NDH1_NuoB.
    InterProi IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
    IPR006138. NADH_UQ_OxRdtase_20Kd_su.
    [Graphical view ]
    Pfami PF01058. Oxidored_q6. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01957. nuoB_fam. 1 hit.
    PROSITEi PS01150. COMPLEX1_20K. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE.
      Strain: 16MImported.

    Entry informationi

    Entry nameiD0B2M2_BRUME
    AccessioniPrimary (citable) accession number: D0B2M2
    Entry historyi
    Integrated into UniProtKB/TrEMBL: November 24, 2009
    Last sequence update: November 24, 2009
    Last modified: October 1, 2014
    This is version 32 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)