Periplasmic nitrate reductase - Salmonella typhimurium (strain D23580)

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C9XA97 (C9XA97_SALTD) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 25. History...

Clusters with 100%, 90%, 50% identity |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info Customize order

Names and origin

Protein names

Recommended name:
Periplasmic nitrate reductase HAMAP-Rule MF_01630
EC=1.7.99.4 HAMAP-Rule MF_01630

Gene names

Name:	napA HAMAP-Rule MF_01630
Ordered Locus Names:	STMMW_22841 EMBL CBG25295.1

Organism

Salmonella typhimurium (strain D23580) [Complete proteome] [HAMAP] EMBL CBG25295.1

Taxonomic identifier

568708 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella ›

Protein attributes

Sequence length	828 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630 SAAS SAAS006656
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630 SAAS SAAS006656
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630 SAAS SAAS006656 Binds 1 molybdenum ion per subunit By similarity. HAMAP-Rule MF_01630 SAAS SAAS006656 Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity. HAMAP-Rule MF_01630 SAAS SAAS006656
Subunit structure	Interacts with NapB By similarity. HAMAP-Rule MF_01630 SAAS SAAS006656
Subcellular location	Periplasm By similarity HAMAP-Rule MF_01630.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven By similarity. HAMAP-Rule MF_01630
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily. HAMAP-Rule MF_01630

Ontologies

Keywords
Biological process	Electron transport HAMAP-Rule MF_01630 SAAS SAAS006656 Nitrate assimilation HAMAP-Rule MF_01630 SAAS SAAS006656 Transport
Cellular component	Periplasm HAMAP-Rule MF_01630
Domain	Signal HAMAP-Rule MF_01630
Ligand	4Fe-4S HAMAP-Rule MF_01630 SAAS SAAS006656 Iron Iron-sulfur Metal-binding Molybdenum HAMAP-Rule MF_01630 SAAS SAAS006656
Molecular function	Oxidoreductase HAMAP-Rule MF_01630 SAAS SAAS006656
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular_component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP molybdenum ion binding Inferred from electronic annotation. Source: InterPro nitrate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 31

Tat-type signal By similarity HAMAP-Rule MF_01630

Sites

Metal binding

Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_01630

Metal binding

Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_01630

Metal binding

Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_01630

Metal binding

Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_01630

Sequences

Sequence

Length

Mass (Da)

Tools

C9XA97 [UniParc].

Last modified November 24, 2009. Version 1.
Checksum: 0CF99BC8B2509A85
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FASTA

828

92,874

        10         20         30         40         50         60 
MKLSRRSFMK ANAVAAAAAA AGLSVPGVAR AVVGQQEAIK WDKAPCRFCG TGCGVLVGTQ 

        70         80         90        100        110        120 
QGRVVACQGD PDAPVNRGLN CIKGYFLPKI MYGKDRLTQP MLRMKDGSYH KDGEFTPVSW 

       130        140        150        160        170        180 
EQAFDVMEEK FKTSLKEKGP EAIGMFGSGQ WTIWEGYAAA KLFKAGFRSN NIDPNARHCM 

       190        200        210        220        230        240 
ASAVVGFMRT FGMDEPMGCY DDIEQADAFV LWGSNMAEMH PILWSRITNR RLSDPNVKVA 

       250        260        270        280        290        300 
VLSTFQHRSF ELADNGIVFT PQSDLVILNY IANYIIQNNA VNQDFFTKHV NLRKGATDIG 

       310        320        330        340        350        360 
YGLRPTHPLE KAAKNPGSDA SEPMSFDEYK AFVAEYTLDK TAEMTGVPKD QLEQLAQLYA 

       370        380        390        400        410        420 
DPNKRVISYW TMGFNQHTRG VWANNLVYNL HLLTGKISQP GCGPFSLTGQ PSACGTAREV 

       430        440        450        460        470        480 
GTFSHRLPAD MVVTNEKHRD ICEKHWQIPA GTIPAKVGLH AVAQDRALKD GKLNVYWVMC 

       490        500        510        520        530        540 
NNNMQAGPNI NEDRMPGWRD PRNFIIVSDP YPTVSALSAD LILPTAMWVE KEGAYGNAER 

       550        560        570        580        590        600 
RTQFWRQQIK APGEAKSDLW QLVQFSRRFK TEEVWPEALL AQKPELRGKT LYDVLFATPA 

       610        620        630        640        650        660 
VSKFPLSELK EDQLNDESRE LGFYLQKGLF EEYAWFGRGH GHDLAPFDDY HNARGLRWPV 

       670        680        690        700        710        720 
VEGKETQWRY SEGNDPYVKA GEGYKFYGKP DGKAVIFALP FEPAAESPDN EYDLWLSTGR 

       730        740        750        760        770        780 
VLEHWHTGSM TRRVPELHRA FPEAVVFIHP LDAKARDLRR GDKVKVSSRR GEVISIVETR 

       790        800        810        820 
GRNRPPQGLV YMPFFDAAQL VNNLTLDATD PLSKETDFKK CAVKLAKV

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References

[1]

"Epidemic multiple drug resistant Salmonella Typhimurium causing invasive disease in sub-Saharan Africa have a distinct genotype."
Kingsley R.A., Msefula C.L., Thomson N.R., Kariuki S., Holt K.E., Gordon M.A., Harris D., Clarke L., Whitehead S., Sangal V., Marsh K., Achtman M., Molyneux M.E., Cormican M., Parkhill J., Maclennan C.A., Heyderman R.S., Dougan G.
Genome Res. 19:2279-2287(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: D23580 EMBL CBG25295.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FN424405 Genomic DNA. Translation: CBG25295.1.
RefSeq	YP_005233321.1. NC_016854.1.
3D structure databases
ProteinModelPortal	C9XA97.
SMR	C9XA97. Positions 1-35, 37-827.
ModBase	Search...
Proteomic databases
PRIDE	C9XA97.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CBG25295; CBG25295; STMMW_22841.
GeneID	11761312.
KEGG	sev:STMMW_22841.
PATRIC	36725981. VBISalEnt111430_2417.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000031441.
KO	K02567.
Family and domain databases
Gene3D	2.40.40.20. 1 hit.
HAMAP	MF_01630. Nitrate_reduct.
InterPro	IPR009010. Asp_de-COase-like_dom. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4_dom. IPR006655. Mopterin_OxRdtase_prok_CS. IPR010051. Periplasm_NO3_reductase_lsu. IPR006311. TAT_signal. IPR019546. TAT_signal_bac_arc. [Graphical view]
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
SMART	SM00926. Molybdop_Fe4S4. 1 hit. [Graphical view]
SUPFAM	SSF50692. Asp_decarb_fold. 1 hit.
TIGRFAMs	TIGR01706. NAPA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C9XA97_SALTD

Accession

Primary (citable) accession number: C9XA97

Entry history

Integrated into UniProtKB/TrEMBL:	November 24, 2009
Last sequence update:	November 24, 2009
Last modified:	May 1, 2013
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information