C9VMJ5 (C9VMJ5_9RHIZ) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Glutamate 5-kinase HAMAP MF_00456
EC=2.7.2.11 HAMAP MF_00456

Alternative name(s):
Gamma-glutamyl kinase HAMAP MF_00456

Gene names

Name:	proB HAMAP MF_00456
ORF Names:	BAQG_03160 EMBL EEX88197.1

Organism

Brucella ceti B1/94 EMBL EEX88197.1

Taxonomic identifier

520457 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP MF_00456 SAAS SAAS011529
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456 SAAS SAAS011529
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456 SAAS SAAS011529
Subcellular location	Cytoplasm By similarity HAMAP MF_00456 SAAS SAAS011529.
Sequence similarities	Belongs to the glutamate 5-kinase family. HAMAP MF_00456 Contains 1 PUA domain. HAMAP MF_00456 SAAS SAAS011529

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis SAAS SAAS011529 HAMAP MF_00456
Cellular component	Cytoplasm SAAS SAAS011529 HAMAP MF_00456
Ligand	ATP-binding SAAS SAAS011529 HAMAP MF_00456 Nucleotide-binding
Molecular function	Kinase SAAS SAAS011529 HAMAP MF_00456 EMBL EEX88197.1 Transferase
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Domain	279 – 356	78	PUA By similarity HAMAP MF_00456

Sequences

	Sequence		Length	Mass (Da)
	C9VMJ5 [UniParc]. Last modified November 24, 2009. Version 1. Checksum: D4AA35B35182226A	FASTA	378	39,874
	10 20 30 40 50 60 MLKKLKDYRR IVVKIGSALL VDRATGLKRE WLESLGQDIA ALQHAGVEVL VVSSGAIALG 70 80 90 100 110 120 RTVLGLPKKA LKLEESQAAA AAGQIALAKA YADVLGGHGI KSGQILVTLS DTEERRRYLN 130 140 150 160 170 180 ARATIETLLK LKAVPIINEN DTVATTEIRY GDNDRLAARV ATMMGADLLI LLSDIDGLYT 190 200 210 220 230 240 APPHKNPDAQ FLPFVETITP QIEAMAGAAA SELSRGGMKT KLDAGKIANA AGTAMIITSG 250 260 270 280 290 300 TRFGPLSAID RGERATLFEA AHAPVNAWKT WISGNLEPAG RLTVDAGAVK ALKSGKSLLP 310 320 330 340 350 360 AGVKEVDGDF ERGDTVAVMN EDGREIARGL IAYDAADARK VAGHKSDEIS AILGYDARAA 370 MIHRNDLVVR AASDAKAA

References

[1]

"The Genome Sequence of Brucella ceti B1/94." The Broad Institute Genome Sequencing Platform Ward D., Young S.K., Kodira C.D., Zeng Q., Koehrsen M., Alvarado L., Berlin A., Borenstein D., Chen Z., Engels R., Freedman E., Gellesch M., Goldberg J., Griggs A., Gujja S., Heiman D., Hepburn T., Howarth C. , Jen D., Larson L., Lewis B., Mehta T., Park D., Pearson M., Roberts A., Saif S., Shea T., Shenoy N., Sisk P., Stolte C., Sykes S., Walk T., White J., Yandava C., Whatmore A.M., Perrett L.L., O'Callaghan D., Nusbaum C., Galagan J., Birren B. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: B1/94 EMBL EEX88197.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EQ999628 Genomic DNA. Translation: EEX88197.1.
3D structure databases
ProteinModelPortal	C9VMJ5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
PATRIC	24206647. VBIBruCet113143_3371.
Family and domain databases
HAMAP	MF_00456. ProB. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. IPR011529. Glu_5kinase. IPR005715. Glu_5kinase/COase_Synthase. IPR019797. Glutamate_5-kinase_CS. IPR002478. PUA. IPR015947. PUA-like_domain. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
SUPFAM	SSF53633. Aa_kinase. 1 hit. SSF88697. PUA-like. 1 hit.
TIGRFAMs	TIGR01027. ProB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C9VMJ5_9RHIZ

Accession

Primary (citable) accession number: C9VMJ5

Entry history

Integrated into UniProtKB/TrEMBL:	November 24, 2009
Last sequence update:	November 24, 2009
Last modified:	December 14, 2011
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)