C9VKB1 (C9VKB1_9RHIZ) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
LexA repressor HAMAP MF_00015 RuleBase RU003992
EC=3.4.21.88 HAMAP MF_00015 RuleBase RU003992

Gene names

Name:	lexA HAMAP MF_00015
ORF Names:	BAQG_01391 EMBL EEX87547.1

Organism

Brucella ceti B1/94 EMBL EEX87547.1

Taxonomic identifier

520457 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair By similarity. HAMAP MF_00015 Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, leading to derepression of the SOS regulon and eventually DNA repair By similarity. SAAS SAAS006199
Catalytic activity	Hydrolysis of Ala-|-Gly bond in repressor lexA. HAMAP MF_00015 SAAS SAAS006197 RuleBase RU003992
Subunit structure	Homodimer By similarity. HAMAP MF_00015 SAAS SAAS006197
Sequence similarities	Belongs to the peptidase S24 family. HAMAP MF_00015 RuleBase RU003991

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication HAMAP MF_00015 SAAS SAAS006197 RuleBase RU003992 SOS response HAMAP MF_00015 SAAS SAAS006197 RuleBase RU003992 Transcription Transcription regulation HAMAP MF_00015 SAAS SAAS006197 RuleBase RU003992
Ligand	DNA-binding HAMAP MF_00015 SAAS SAAS006197 RuleBase RU003992
Molecular function	Hydrolase HAMAP MF_00015 SAAS SAAS006197 RuleBase RU003991 Repressor HAMAP MF_00015 SAAS SAAS006197 RuleBase RU003992
PTM	Autocatalytic cleavage HAMAP MF_00015 RuleBase RU003991
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP proteolysis Inferred from electronic annotation. Source: InterPro transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: HAMAP serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	DNA binding	26 – 46	21	H-T-H motif By similarity HAMAP MF_00015
Sites
	Active site	161	1	For autocatalytic cleavage activity By similarity HAMAP MF_00015
	Active site	199	1	For autocatalytic cleavage activity By similarity HAMAP MF_00015
	Site	126 – 127	2	Cleavage; by autolysis By similarity HAMAP MF_00015

Sequences

	Sequence		Length	Mass (Da)
	C9VKB1 [UniParc]. Last modified November 24, 2009. Version 1. Checksum: 40661DAC60C8C7A2	FASTA	240	26,070
	10 20 30 40 50 60 MLTRKQHELL LFIHERLKET GIPPSFDEMK EALDLASKSG IHRLITALEE RGFIRRLPNR 70 80 90 100 110 120 ARALEVLRLP DSIAPGLSPQ KKFAPSVIEG SLGKVASVQP VRPAPAPQNS EAPATVSVPV 130 140 150 160 170 180 MGRIAAGVPI SAIQNQTHML SLPPEMIGAG EHYALEVKGD SMIDAGIFDG DTVIIKRGDT 190 200 210 220 230 240 ANPGEIVVAL VDEEEATLKR FRREGASIAL EAANPAYETR IFGPDRVHVQ GKLVGLIRRY

References

[1]

"The Genome Sequence of Brucella ceti B1/94." The Broad Institute Genome Sequencing Platform Ward D., Young S.K., Kodira C.D., Zeng Q., Koehrsen M., Alvarado L., Berlin A., Borenstein D., Chen Z., Engels R., Freedman E., Gellesch M., Goldberg J., Griggs A., Gujja S., Heiman D., Hepburn T., Howarth C. , Jen D., Larson L., Lewis B., Mehta T., Park D., Pearson M., Roberts A., Saif S., Shea T., Shenoy N., Sisk P., Stolte C., Sykes S., Walk T., White J., Yandava C., Whatmore A.M., Perrett L.L., O'Callaghan D., Nusbaum C., Galagan J., Birren B. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: B1/94 EMBL EEX87547.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EQ999621 Genomic DNA. Translation: EEX87547.1.
3D structure databases
ProteinModelPortal	C9VKB1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
PATRIC	24205201. VBIBruCet113143_2339.
Family and domain databases
HAMAP	MF_00015. LexA. [Tree]
InterPro	IPR006199. LexA_DNA-bd_dom. IPR006200. Pept_S24_LexA. IPR006197. Peptidase_S24_LexA. IPR019759. Peptidase_S24_S26. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF01726. LexA_DNA_bind. 1 hit. PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00726. LEXASERPTASE.
SUPFAM	SSF51306. Pept_S24_S26_C. 1 hit.
TIGRFAMs	TIGR00498. LexA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

C9VKB1_9RHIZ

Accession

Primary (citable) accession number: C9VKB1

Entry history

Integrated into UniProtKB/TrEMBL:	November 24, 2009
Last sequence update:	November 24, 2009
Last modified:	January 25, 2012
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)