C9VCS9 (C9VCS9_BRUNE) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Adenosylhomocysteinase HAMAP MF_00563 RuleBase RU000548
EC=3.3.1.1 HAMAP MF_00563 RuleBase RU000548

Alternative name(s):
S-adenosyl-L-homocysteine hydrolase HAMAP MF_00563

Gene names

Name:	ahcY HAMAP MF_00563
ORF Names:	BANG_00225 EMBL EEY03514.1

Organism

Brucella neotomae 5K33 EMBL EEY03514.1

Taxonomic identifier

520456 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May play a key role in the regulation of the intracellular concentration of adenosylhomocysteine By similarity. HAMAP MF_00563
Catalytic activity	S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine. HAMAP MF_00563 RuleBase RU000548
Cofactor	Binds 1 NAD per subunit By similarity. HAMAP MF_00563 NAD By similarity. RuleBase RU000548
Pathway	Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. HAMAP MF_00563 Amino-acid biosynthesis; homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. RuleBase RU000548
Subcellular location	Cytoplasm By similarity HAMAP MF_00563.
Sequence similarities	Belongs to the adenosylhomocysteinase family. HAMAP MF_00563 RuleBase RU004166

Ontologies

Keywords
Biological process	One-carbon metabolism HAMAP MF_00563 RuleBase RU000548
Cellular component	Cytoplasm HAMAP MF_00563
Ligand	NAD HAMAP MF_00563 RuleBase RU000548
Molecular function	Hydrolase HAMAP MF_00563 RuleBase RU000548
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenosylhomocysteinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	193 – 195	3	NAD By similarity HAMAP MF_00563
	Nucleotide binding	256 – 261	6	NAD By similarity HAMAP MF_00563
	Nucleotide binding	335 – 337	3	NAD By similarity HAMAP MF_00563
Sites
	Binding site	57	1	Substrate By similarity PIRSR PIRSR001109-1 HAMAP MF_00563
	Binding site	132	1	Substrate By similarity PIRSR PIRSR001109-1 HAMAP MF_00563
	Binding site	192	1	Substrate By similarity PIRSR PIRSR001109-1 HAMAP MF_00563
	Binding site	222	1	Substrate By similarity PIRSR PIRSR001109-1 HAMAP MF_00563
	Binding site	226	1	Substrate By similarity PIRSR PIRSR001109-1 HAMAP MF_00563
	Binding site	227	1	NAD By similarity HAMAP MF_00563
	Binding site	279	1	NAD By similarity HAMAP MF_00563
	Binding site	314	1	NAD By similarity HAMAP MF_00563
	Binding site	380	1	NAD By similarity HAMAP MF_00563

Sequences

	Sequence		Length	Mass (Da)
	C9VCS9 [UniParc]. Last modified November 24, 2009. Version 1. Checksum: 6AB3B4B1C2F0BE41	FASTA	466	50,791
	10 20 30 40 50 60 MTASQDFVVK DISLADWGRK ELDIAETEMP GLMAAREEFG KSQPLKGARI SGSLHMTIQT 70 80 90 100 110 120 AVLIETLKVL GAEVRWASCN IFSTQDHAAA AIAATGTPVF AVKGETLEEY WTYTDQIFQW 130 140 150 160 170 180 PDGEPSNMIL DDGGDATMYI LIGARAEAGE DVLSNPQSEE EEVLFAQIKK RMAATPGFFT 190 200 210 220 230 240 KQRAAIKGVT EETTTGVNRL YQLQKKGLLP FPAINVNDSV TKSKFDNKYG CKESLVDGIR 250 260 270 280 290 300 RGTDVMMAGK VAVVCGYGDV GKGSAQSLAG AGARVKVTEV DPICALQAAM DGFEVVTLDD 310 320 330 340 350 360 AASTADIVVT TTGNKDVITI DHMRKMKDMC IVGNIGHFDN EIQVAALRNL KWTNVKPQVD 370 380 390 400 410 420 LIEFPDGKRL ILLSEGRLLN LGNATGHPSF VMSASFTNQV LGQIELFTRT DAYKNEVYVL 430 440 450 460 PKHLDEKVAR LHLDKLGAKL TVLSEEQAAY IGVTPQGPFK SEHYRY

References

[1]

"The Genome Sequence of Brucella neotomae 5K33." The Broad Institute Genome Sequencing Platform Ward D., Young S.K., Kodira C.D., Zeng Q., Koehrsen M., Alvarado L., Berlin A., Borenstein D., Chen Z., Engels R., Freedman E., Gellesch M., Goldberg J., Griggs A., Gujja S., Heiman D., Hepburn T., Howarth C. , Jen D., Larson L., Lewis B., Mehta T., Park D., Pearson M., Roberts A., Saif S., Shea T., Shenoy N., Sisk P., Stolte C., Sykes S., Walk T., White J., Yandava C., Whatmore A.M., Perrett L.L., O'Callaghan D., Nusbaum C., Galagan J., Birren B. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: 5K33 EMBL EEY03514.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EQ999582 Genomic DNA. Translation: EEY03514.1.
3D structure databases
ProteinModelPortal	C9VCS9.
SMR	C9VCS9. Positions 5-466.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
PATRIC	24244631. VBIBruNeo114381_3056.
Family and domain databases
HAMAP	MF_00563. AdoHcyase. [Tree]
InterPro	IPR000043. Adenosylhomocysteinase. IPR015878. Ado_hCys_hydrolase_NAD-bd. IPR020082. S-Ado-L-homoCys_hydrolase_CS. [Graphical view]
PANTHER	PTHR23420. Ad_hcy_hydrolase. 1 hit.
Pfam	PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view]
PIRSF	PIRSF001109. Ad_hcy_hydrolase. 1 hit.
SMART	SM00996. AdoHcyase. 1 hit. SM00997. AdoHcyase_NAD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00936. AhcY. 1 hit.
PROSITE	PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C9VCS9_BRUNE

Accession

Primary (citable) accession number: C9VCS9

Entry history

Integrated into UniProtKB/TrEMBL:	November 24, 2009
Last sequence update:	November 24, 2009
Last modified:	January 25, 2012
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)