C9UV30 (C9UV30_BRUAO) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha chain HAMAP MF_00281
EC=6.1.1.20 HAMAP MF_00281

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha chain HAMAP MF_00281

Gene names

Name:	pheS HAMAP MF_00281
ORF Names:	BADG_00251 EMBL EEX57997.1

Organism

Brucella abortus bv. 2 str. 86/8/59 EMBL EEX57997.1

Taxonomic identifier

520450 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00281
Subunit structure	Tetramer of two alpha and two beta chains By similarity. HAMAP MF_00281
Subcellular location	Cytoplasm By similarity HAMAP MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily. HAMAP MF_00281

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP MF_00281
Cellular component	Cytoplasm HAMAP MF_00281
Ligand	ATP-binding HAMAP MF_00281 Magnesium HAMAP MF_00281 Metal-binding HAMAP MF_00281 Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase HAMAP MF_00281 EMBL EEX57997.1 Ligase
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	C9UV30 [UniParc]. Last modified November 24, 2009. Version 1. Checksum: BC2B822DB0270926	FASTA	369	41,598
	10 20 30 40 50 60 MSDLEQLERQ ILEDIAAAVD EQGIEAVRVA ALGKKGTVSE KLKTLGGMSP EERQMQGPAI 70 80 90 100 110 120 NGLKNRVTEA LSERRTELRK AAVAARLERE KVDVTLPVRE SAASRGRIHP ISQVIDEITA 130 140 150 160 170 180 IFADMGFSIA EGPDIETDYY NFTALNFPEG HPAREMHDTF FFNPDEKGER KLLRTHTSPV 190 200 210 220 230 240 QVHTMEKFAA MRDKEGRDEP IRIVIPGKTY RMDSDATHSP MFHQVEGLVV DKSANVANMK 250 260 270 280 290 300 WVLEEFCKAF FEVPSVKMRM RPSFFPFTEP SVEVDIQCDR SGPHVKFGEG NDWLEILGCG 310 320 330 340 350 360 MVHPNVLRMS GYDPEVYQGF AWGMGIDRIA MLKYGMPDLR AFFDADVRWI EHYGFRPLDI PTLFGGLSA

References

[1]

"The Genome Sequence of Brucella abortus bv. 2 str. 86/8/59." The Broad Institute Genome Sequencing Platform Ward D., Young S.K., Kodira C.D., Zeng Q., Koehrsen M., Alvarado L., Berlin A., Borenstein D., Chen Z., Engels R., Freedman E., Gellesch M., Goldberg J., Griggs A., Gujja S., Heiman D., Hepburn T., Howarth C. , Jen D., Larson L., Lewis B., Mehta T., Park D., Pearson M., Roberts A., Saif S., Shea T., Shenoy N., Sisk P., Stolte C., Sykes S., Walk T., White J., Yandava C., Whatmore A.M., Perrett L.L., O'Callaghan D., Nusbaum C., Galagan J., Birren B. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: 86/8/59 EMBL EEX57997.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS999900 Genomic DNA. Translation: EEX57997.1.
3D structure databases
ProteinModelPortal	C9UV30.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
PATRIC	24164866. VBIBruAbo60950_2097.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR022911. Phe_tRNA_synth_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. PheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C9UV30_BRUAO

Accession

Primary (citable) accession number: C9UV30

Entry history

Integrated into UniProtKB/TrEMBL:	November 24, 2009
Last sequence update:	November 24, 2009
Last modified:	January 25, 2012
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)