D-alanine--D-alanine ligase - Brucella pinnipedialis (strain NCTC 12890 / BCCN 94-73 / B2/94)

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C9TXE7 (C9TXE7_BRUPB) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 21. History...

Clusters with 100%, 90%, 50% identity |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info Customize order

Names and origin

Protein names

Recommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047
EC=6.3.2.4 HAMAP-Rule MF_00047

Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047

Gene names

Name:	ddl HAMAP-Rule MF_00047 EMBL AEK54733.1
ORF Names:	BAHG_01690 EMBL EEY00761.1, BPI_I1480 EMBL AEK54733.1

Organism

Brucella pinnipedialis (strain NCTC 12890 / BCCN 94-73 / B2/94) [Complete proteome] [HAMAP] EMBL EEY00761.1

Taxonomic identifier

520461 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella ›

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. SAAS SAAS005905 HAMAP-Rule MF_00047
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. SAAS SAAS005905 HAMAP-Rule MF_00047
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity. SAAS SAAS005905
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. SAAS SAAS005905 HAMAP-Rule MF_00047
Subcellular location	Cytoplasm By similarity SAAS SAAS005905 HAMAP-Rule MF_00047.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047 Contains 1 ATP-grasp domain. SAAS SAAS005905 HAMAP-Rule MF_00047

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation SAAS SAAS005905 HAMAP-Rule MF_00047 Peptidoglycan synthesis SAAS SAAS005905 HAMAP-Rule MF_00047
Cellular component	Cytoplasm SAAS SAAS005905 HAMAP-Rule MF_00047
Ligand	ATP-binding SAAS SAAS005905 HAMAP-Rule MF_00047 Magnesium SAAS SAAS005905 HAMAP-Rule MF_00047 Manganese SAAS SAAS005905 HAMAP-Rule MF_00047 Metal-binding SAAS SAAS005905 HAMAP-Rule MF_00047 Nucleotide-binding
Molecular function	Ligase SAAS SAAS005905 HAMAP-Rule MF_00047 EMBL EEY00761.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Regions

Domain

102 – 302

201

ATP-grasp By similarity HAMAP-Rule MF_00047

Nucleotide binding

128 – 183

ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding

252

Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047

Metal binding

269

Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047

Metal binding

269

Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Metal binding

271

Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence

Length

Mass (Da)

Tools

C9TXE7 [UniParc].

Last modified November 24, 2009. Version 1.
Checksum: 633111E4CB6596F8
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FASTA

308

33,394

        10         20         30         40         50         60 
MTGKHVAVLM GGFSSERSVS LSSGVACATT LEECGYRVTR IDVDRNVASI LVELKPDVVF 

        70         80         90        100        110        120 
NALHGPFGED GAIQGVLEYL QIPYTHSGVL ASALAMDKDR AKKVAAAAGV VVAPSRLMNR 

       130        140        150        160        170        180 
FDIGSQHPMK PPYVVKPVRE GSSFGVVIVK EDQPHPPQVI GSADWKYGDE VMVEGYIAGR 

       190        200        210        220        230        240 
ELTCAVMGDR AMDVCEIIPV GYQFYDYDSK YVAGASTHVC PAKILPNIYQ KIQTMALTAH 

       250        260        270        280        290        300 
RAIGCRGVSR SDFRFDDRFS EEGEVVWLEI NTQPGMTPTS LVPDIAKAAG ISFAELLSWM 


VEDASCLR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Genome Sequence of Brucella pinnipedialis B2/94." The Broad Institute Genome Sequencing Platform Ward D., Young S.K., Kodira C.D., Zeng Q., Koehrsen M., Alvarado L., Berlin A., Borenstein D., Chen Z., Engels R., Freedman E., Gellesch M., Goldberg J., Griggs A., Gujja S., Heiman D., Hepburn T., Howarth C. Birren B. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: B2/94 EMBL EEY00761.1.
[2]	Zygmunt M., Clockaert A. Submitted (JUN-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: B2/94 EMBL AEK54733.1.
[3]	"The genome sequence of Brucella pinnipedialis B2/94 sheds light on the evolutionary history of the genus Brucella." Audic S., Lescot M., Claverie J.M., Cloeckaert A., Zygmunt M.S. BMC Evol. Biol. 11:200-200(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B2/94 EMBL AEK54733.1 and NCTC 12890 / BCCN 94-73 / B2/94.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002078 Genomic DNA. Translation: AEK54733.1. DS999848 Genomic DNA. Translation: EEY00761.1.
RefSeq	YP_004756501.1. NC_015857.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AEK54733; AEK54733; BPI_I1480. EEY00761; EEY00761; BAHG_01690.
GeneID	10997957.
KEGG	bpp:BPI_I1480.
PATRIC	24252781. VBIBruPin17457_1509.
Organism-specific databases
CMR	Search...
Phylogenomic databases
KO	K01921.
Enzyme and pathway databases
BioCyc	BPIN520461:GJF0-1478-MONOMER.
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 2 hits. 3.40.50.20. 1 hit.
HAMAP	MF_00047. Dala_Dala_lig.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR016185. PreATP-grasp_dom. [Graphical view]
PANTHER	PTHR23132. PTHR23132. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 2 hits. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C9TXE7_BRUPB

Accession

Primary (citable) accession number: C9TXE7

Entry history

Integrated into UniProtKB/TrEMBL:	November 24, 2009
Last sequence update:	November 24, 2009
Last modified:	May 1, 2013
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information