Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

C9SB49

- MAP2_VERA1

UniProt

C9SB49 - MAP2_VERA1

Protein

Methionine aminopeptidase 2

Gene

VDBG_01708

Organism
Verticillium alfalfae (strain VaMs.102 / ATCC MYA-4576 / FGSC 10136) (Verticillium wilt of alfalfa) (Verticillium albo-atrum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 35 (01 Oct 2014)
      Sequence version 1 (24 Nov 2009)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniRule annotation

    Catalytic activityi

    Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

    Cofactori

    Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei196 – 1961SubstrateUniRule annotation
    Metal bindingi216 – 2161Divalent metal cation 1UniRule annotation
    Metal bindingi227 – 2271Divalent metal cation 1UniRule annotation
    Metal bindingi227 – 2271Divalent metal cation 2; catalyticUniRule annotation
    Metal bindingi296 – 2961Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation
    Binding sitei304 – 3041SubstrateUniRule annotation
    Metal bindingi329 – 3291Divalent metal cation 2; catalyticUniRule annotation
    Metal bindingi423 – 4231Divalent metal cation 1UniRule annotation
    Metal bindingi423 – 4231Divalent metal cation 2; catalyticUniRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-HAMAP
    2. metalloaminopeptidase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. protein initiator methionine removal Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminopeptidase, Hydrolase, Protease

    Keywords - Ligandi

    Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methionine aminopeptidase 2UniRule annotation (EC:3.4.11.18UniRule annotation)
    Short name:
    MAP 2UniRule annotation
    Short name:
    MetAP 2UniRule annotation
    Alternative name(s):
    Peptidase MUniRule annotation
    Gene namesi
    ORF Names:VDBG_01708
    OrganismiVerticillium alfalfae (strain VaMs.102 / ATCC MYA-4576 / FGSC 10136) (Verticillium wilt of alfalfa) (Verticillium albo-atrum)
    Taxonomic identifieri526221 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeGlomerellalesPlectosphaerellaceaemitosporic PlectosphaerellaceaeVerticillium
    ProteomesiUP000008698: Unassembled WGS sequence

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 442442Methionine aminopeptidase 2PRO_0000407672Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliC9SB49.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi68 – 714Poly-Lys

    Sequence similaritiesi

    Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.UniRule annotation

    Phylogenomic databases

    KOiK01265.
    OrthoDBiEOG7BGHW3.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    3.90.230.10. 2 hits.
    HAMAPiMF_03175. MetAP_2_euk.
    InterProiIPR001714. Pept_M24_MAP.
    IPR000994. Pept_M24_structural-domain.
    IPR002468. Pept_M24A_MAP2.
    IPR018349. Pept_M24A_MAP2_BS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PANTHERiPTHR10804:SF9. PTHR10804:SF9. 1 hit.
    PfamiPF00557. Peptidase_M24. 1 hit.
    [Graphical view]
    PRINTSiPR00599. MAPEPTIDASE.
    SUPFAMiSSF55920. SSF55920. 2 hits.
    TIGRFAMsiTIGR00501. met_pdase_II. 1 hit.
    PROSITEiPS01202. MAP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C9SB49-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAQAPTEAL KELQVGEAAT TNGKEAPDGN AGRQRQRRLG GRGRCGPRCS    50
    PAVRVPLRRR RRRRRTRKKK KAPTAQSDPP RVLMSQLFPQ KNYPVGEECE 100
    YLNENAYRTT DEEKRHLDNL NSDFLTDYRE AAEIHRQVRQ WAQKNIKPGQ 150
    GLTEIAEGIE DGVRALTGHW GLEEGDALKG GMGFPTGLSI NHCAAHYTPN 200
    LGNKMVLQQE DVMKVDFGVH VNGRIVDSAF TLAFEPKYDN LLTAVREATN 250
    AGVKEAGIDV RVGDIGGVIQ EVMESYEVEI DGTTYPVKSI RNLNGHTIER 300
    WSIHGAKSVP IVKSNDTTKM EEGDVFAVET FGSTGNGYVR DDMEVSHYAK 350
    RGDSNVPLRL DSAKRLLNVI NKNFGTLPFC RRYLDRLGQE KYLLGLNNLV 400
    SAGIVEAYPP LVDKKGSYTA QFEHTILIRP TVKEVISRGE DY 442
    Length:442
    Mass (Da):49,263
    Last modified:November 24, 2009 - v1
    Checksum:i7A0DC345FFFCB7FB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS985215 Genomic DNA. Translation: EEY15599.1.
    RefSeqiXP_003007520.1. XM_003007474.1.

    Genome annotation databases

    GeneIDi9533849.
    KEGGival:VDBG_01708.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS985215 Genomic DNA. Translation: EEY15599.1 .
    RefSeqi XP_003007520.1. XM_003007474.1.

    3D structure databases

    ProteinModelPortali C9SB49.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 9533849.
    KEGGi val:VDBG_01708.

    Phylogenomic databases

    KOi K01265.
    OrthoDBi EOG7BGHW3.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    3.90.230.10. 2 hits.
    HAMAPi MF_03175. MetAP_2_euk.
    InterProi IPR001714. Pept_M24_MAP.
    IPR000994. Pept_M24_structural-domain.
    IPR002468. Pept_M24A_MAP2.
    IPR018349. Pept_M24A_MAP2_BS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    PANTHERi PTHR10804:SF9. PTHR10804:SF9. 1 hit.
    Pfami PF00557. Peptidase_M24. 1 hit.
    [Graphical view ]
    PRINTSi PR00599. MAPEPTIDASE.
    SUPFAMi SSF55920. SSF55920. 2 hits.
    TIGRFAMsi TIGR00501. met_pdase_II. 1 hit.
    PROSITEi PS01202. MAP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: VaMs.102 / ATCC MYA-4576 / FGSC 10136.

    Entry informationi

    Entry nameiMAP2_VERA1
    AccessioniPrimary (citable) accession number: C9SB49
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 3, 2011
    Last sequence update: November 24, 2009
    Last modified: October 1, 2014
    This is version 35 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3