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C9RMQ5 (C9RMQ5_FIBSS) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Pyridoxine 5'-phosphate synthase HAMAP-Rule MF_00279
Short name=PNP synthase HAMAP-Rule MF_00279
EC=2.6.99.2 HAMAP-Rule MF_00279
Gene names
Name:pdxJ HAMAP-Rule MF_00279
Ordered Locus Names:Fisuc_0671, FSU_1107
OrganismFibrobacter succinogenes (strain ATCC 19169 / S85) [Complete proteome] [HAMAP] EMBL ACX74283.1
Taxonomic identifier59374 [NCBI]
Taxonomic lineageBacteria › Fibrobacteres › Fibrobacterales › Fibrobacteraceae › Fibrobacter ›

Protein attributes

Sequence length242 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate By similarity. HAMAP-Rule MF_00279

Catalytic activity

1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate = pyridoxine 5'-phosphate + phosphate + 2 H2O. HAMAP-Rule MF_00279 SAAS SAAS004569

Pathway

Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 5/5. HAMAP-Rule MF_00279 SAAS SAAS004569

Subunit structure

Homooctamer; tetramer of dimers By similarity. HAMAP-Rule MF_00279 SAAS SAAS004569

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00279 SAAS SAAS004569.

Sequence similarities

Belongs to the PNP synthase family. HAMAP-Rule MF_00279

Ontologies

Keywords
   Biological processPyridoxine biosynthesis HAMAP-Rule MF_00279 SAAS SAAS004569
   Cellular componentCytoplasm SAAS SAAS004569 HAMAP-Rule MF_00279
   Molecular functionTransferase HAMAP-Rule MF_00279 SAAS SAAS004569 EMBL ACX74283.1
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processpyridoxine biosynthetic process

Inferred from electronic annotation. Source: HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionpyridoxine 5'-phosphate synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Region10 – 1121-deoxy-D-xylulose 5-phosphate binding By similarity HAMAP-Rule MF_00279
Region217 – 21823-amino-2-oxopropyl phosphate binding By similarity HAMAP-Rule MF_00279

Sites

Active site441Proton acceptor By similarity HAMAP-Rule MF_00279
Active site711Proton acceptor By similarity HAMAP-Rule MF_00279
Binding site813-amino-2-oxopropyl phosphate By similarity HAMAP-Rule MF_00279
Binding site1913-amino-2-oxopropyl phosphate By similarity HAMAP-Rule MF_00279
Binding site4611-deoxy-D-xylulose 5-phosphate By similarity HAMAP-Rule MF_00279
Binding site5111-deoxy-D-xylulose 5-phosphate By similarity HAMAP-Rule MF_00279
Binding site19613-amino-2-oxopropyl phosphate; via amide nitrogen By similarity HAMAP-Rule MF_00279
Site1521Transition state stabilizer By similarity HAMAP-Rule MF_00279

Sequences

Sequence LengthMass (Da)Tools
C9RMQ5 [UniParc].

Last modified November 24, 2009. Version 1.
Checksum: 0D195DFDE62DC9AA

FASTA24226,607
        10         20         30         40         50         60 
MSTKLGVNVD HIATIREARK GKEPDPVAAA MIAELAGCNG ITAHLREDKR HIQDRDIRLL 

        70         80         90        100        110        120 
RGTVTTKLNL EMAPTQAMVQ FAINRQPDMV TLVPEVHTEL STEDGLNVSA KIDELAKYIM 

       130        140        150        160        170        180 
TLRNNDIQVS VFIDAETEQV KAAKKVGANY VEFNTGKYAT AFELGSREEV DREISALQDM 

       190        200        210        220        230        240 
TVLAHKYGLN VLAGRGLNYR NVEAVAQIDG IDEIIIGHSI VSRAALVGME RAVKEMIEAI 


RG

« Hide

References

« Hide 'large scale' references
[1]"Complete sequence of Fibrobacter succinogenes subsp. succinogenes S85."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Detter J.C., Han C., Tapia R., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Weimer P.J. expand/collapse author list , Stevenson D.M., Boyum J., Brumm P.I., Mead D.
Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 19169 / S85.
[2]"Complete sequence of Fibrobacter succinogenes subsp. succinogenes S85."
Durkin A.S., Nelson K.E., Morrison M., Forsberg C.W., Wilson D.B., Russell J.B., Cann I.K.O., Mackie R.I., White B.A.
Submitted (AUG-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 19169 / S85.
[3]Durkin A.S., Nelson K.E., Morrison M., Forsberg C.W., Wilson D.B., Russell J.B., Cann I.K.O., Mackie R.I., White B.A.
Submitted (AUG-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: S85.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001792 Genomic DNA. Translation: ACX74283.1.
CP002158 Genomic DNA. Translation: ADL25097.1.
RefSeqYP_003248765.1. NC_013410.1.
YP_005821159.1. NC_017448.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRING59374.Fisuc_0671.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACX74283; ACX74283; Fisuc_0671.
ADL25097; ADL25097; FSU_1107.
GeneID12432709.
8521269.
KEGGfsc:FSU_1107.
fsu:Fisuc_0671.
PATRIC32141139. VBIFibSuc28982_0664.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0854.
HOGENOMHOG000258094.
KOK03474.
OMALHYHNVK.

Enzyme and pathway databases

UniPathwayUPA00244; UER00313.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00279. PdxJ.
InterProIPR013785. Aldolase_TIM.
IPR004569. PyrdxlP_synth_PdxJ.
[Graphical view]
PfamPF03740. PdxJ. 1 hit.
[Graphical view]
SUPFAMSSF63892. PyrdxlP_synth_PdxJ. 1 hit.
TIGRFAMsTIGR00559. pdxJ. 1 hit.
ProtoNetSearch...

Entry information

Entry nameC9RMQ5_FIBSS
AccessionPrimary (citable) accession number: C9RMQ5
Entry history
Integrated into UniProtKB/TrEMBL: November 24, 2009
Last sequence update: November 24, 2009
Last modified: May 1, 2013
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info