C9RH82 (C9RH82_METVM) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase HAMAP MF_00193 RuleBase RU003812
EC=6.3.1.5 HAMAP MF_00193 RuleBase RU003812

Gene names

Name:	nadE HAMAP MF_00193
Ordered Locus Names:	Metvu_1076

Organism

Methanocaldococcus vulcanius (strain ATCC 700851 / DSM 12094 / M7) (Methanococcus vulcanius) [Complete proteome] [HAMAP]

Taxonomic identifier

579137 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193 RuleBase RU003812
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. RuleBase RU004252 HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family. HAMAP MF_00193 RuleBase RU003811

Ontologies

Keywords
Ligand	ATP-binding HAMAP MF_00193 RuleBase RU003811 NAD HAMAP MF_00193 RuleBase RU003811 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00193 RuleBase RU003811 EMBL ACX72934.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	27 – 34	8	ATP By similarity HAMAP MF_00193
Sites
	Active site	29	1	By similarity HAMAP MF_00193

Sequences

	Sequence		Length	Mass (Da)
	C9RH82 [UniParc]. Last modified November 24, 2009. Version 1. Checksum: 0ACD6AB570D22C85	FASTA	251	28,023
	10 20 30 40 50 60 MKNEVDKIVK FIRDKVEEAR VNGVVIGLSG GIDSSLTAYL CVKALGKDRV LGIIMPEKYS 70 80 90 100 110 120 NRKDIEHAIM VAKSLGIKYI ISDITDILKA FGAGGYVPTR EFDKIADGNL KARIRMCILY 130 140 150 160 170 180 YFANKYNLLV AGTANKSETY VGYGTKHGDI ACDIKPIGNL FKTEVREMAK YLGIPKEIIE 190 200 210 220 230 240 KPPSAGLWEG QTDEDELGVK YETLDKILKC YEEGMNADEV VKKLGVEKEV VDKIFNLIKK 250 NEHKRTLPPT P

References

[1]

"Complete sequence of chromosome of Methanocaldococcus vulcanius M7." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Lcollab F.I., Brettin T., Detter J.C., Han C., Tapia R., Kuske C.R., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Ovchinikova G., Sieprawska-Lupa M., Whitman W.B., Woyke T. Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700851 / DSM 12094 / M7.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001787 Genomic DNA. Translation: ACX72934.1.
RefSeq	YP_003247416.1. NC_013407.1.
3D structure databases
ProteinModelPortal	C9RH82.
ModBase	Search...
Protein-protein interaction databases
STRING	C9RH82.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	8513415.
GenomeReviews	Gene locus Metvu_1076 in contig CP001787_GR.
KEGG	mvu:Metvu_1076.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	DGAVDCH.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

C9RH82_METVM

Accession

Primary (citable) accession number: C9RH82

Entry history

Integrated into UniProtKB/TrEMBL:	November 24, 2009
Last sequence update:	November 24, 2009
Last modified:	December 14, 2011
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)