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C9RF77 (C9RF77_METVM) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 16. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Alanine--tRNA ligase HAMAP MF_00036
EC=6.1.1.7 HAMAP MF_00036
Alternative name(s):
Alanyl-tRNA synthetase HAMAP MF_00036
Gene names
Name:alaS HAMAP MF_00036
Ordered Locus Names:Metvu_0364
OrganismMethanocaldococcus vulcanius (strain ATCC 700851 / DSM 12094 / M7) (Methanococcus vulcanius) [Complete proteome] [HAMAP]
Taxonomic identifier579137 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length895 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036

Catalytic activity

ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036 SAAS SAAS022429

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036 SAAS SAAS022429

Subcellular location

Cytoplasm By similarity HAMAP MF_00036.

Domain

Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family. HAMAP MF_00036

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding5931Zinc By similarity HAMAP MF_00036
Metal binding5971Zinc By similarity HAMAP MF_00036
Metal binding7021Zinc By similarity HAMAP MF_00036
Metal binding7061Zinc By similarity HAMAP MF_00036

Sequences

Sequence LengthMass (Da)Tools
C9RF77 [UniParc].

Last modified November 24, 2009. Version 1.
Checksum: 89D4675F9CEB1E3D

FASTA895103,116
        10         20         30         40         50         60 
MKHNYKVKLF DELGFVRKKC KKCGQWFWTL DEERETCGDA PCDIYSFIGK PITKKPYDYK 

        70         80         90        100        110        120 
EMVKEFINFF KEHGHEPIKR APVTARRWRD DILLTIASIA VFQPWITKGI VKPKANPLVI 

       130        140        150        160        170        180 
AQPCIRLNDI DNVGRTGRHL TCFTMGGHHA FNREDDFKYW QDETVELCFN FFKKLGIDEK 

       190        200        210        220        230        240 
SITFIESWWE GGGNAGPCYE VITHGVELAT LVFMQYEKVG DNYKEIPLKI VDTGYGIERF 

       250        260        270        280        290        300 
VWASTGEPTI YDAIFKNIVN KLKEDAGVKD IDKEILAKIT EVAGLMDVKD VGDLRKLREE 

       310        320        330        340        350        360 
VAKKVNIQVE DLDKLISPYE DIYAIADHTR ALAFMLGDGI VPSNVKDGYL VRMLIRKTLR 

       370        380        390        400        410        420 
HMDRLNLSTP ITEIVAMQLN ELKDLYPELL DMEDYIMEIL ELEEDKYRQT IERGKGIVER 

       430        440        450        460        470        480 
LLKNKKDIDL ENLIELYDSH GLPPEIVKDV AKSLGKDVKI PDNFYTIVAE RHENKKEVKE 

       490        500        510        520        530        540 
KIKLPEVDVD KTELLFYEYP KMKEFEAKVL KVADDYVILD KTAFYPEGGG QKADTGYIIK 

       550        560        570        580        590        600 
GDKKFRVIDV QKENNIVYHK IEGLNDDINE LKEGDIVKGV IDWDRRLSLM RNHTATHVIN 

       610        620        630        640        650        660 
AAAQKVLGRH IWQAGSDVDV DKARLDITHY KRISREELKE IERVANEIVL NNYNVKSIFM 

       670        680        690        700        710        720 
DRNEAEEKFG FRIYQGGVVP GNVLRIVIIE DENGNIVDVQ ACGGTHCQNT GEIGFIKIIK 

       730        740        750        760        770        780 
TERVQDGVER LIYSSGLSAL KAVQEMEDIL EESAEILRCP SEELPKVIKR FFEEWKEQRK 

       790        800        810        820        830        840 
KIEELEKKIG ELKKFELMNK FERVGNYKVL VEKVEANPKE MLNIADNLAT ENTIVVLLND 

       850        860        870        880        890 
KGNILCKRGE NVDIKMNELI RYIAKGGGRE HLAQGKYEGE VEGIKKKVIE FIKNK

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References

[1]"Complete sequence of chromosome of Methanocaldococcus vulcanius M7."
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Lcollab F.I., Brettin T., Detter J.C., Han C., Tapia R., Kuske C.R., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Ovchinikova G., Sieprawska-Lupa M., Whitman W.B., Woyke T.
Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700851 / DSM 12094 / M7.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001787 Genomic DNA. Translation: ACX72229.1.
RefSeqYP_003246711.1. NC_013407.1.

3D structure databases

ProteinModelPortalC9RF77.
ModBaseSearch...

Protein-protein interaction databases

STRINGC9RF77.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID8512696.
GenomeReviewsGene locus Metvu_0364 in contig CP001787_GR.
KEGGmvu:Metvu_0364.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAMFTNSGM.

Family and domain databases

HAMAPMF_00036_A. Ala_tRNA_synth_A.
[Tree]
InterProIPR002318. Ala-tRNA-synth_IIc.
IPR018162. Ala-tRNA-synth_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR022429. Ala-tRNA_synth_arc.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR012947. tRNA_SAD.
[Graphical view]
KOK01872.
PANTHERPTHR11777:SF6. PTHR11777:SF6. 1 hit.
PfamPF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSPR00980. TRNASYNTHALA.
SMARTSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMSSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit.
SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMsTIGR03683. A-tRNA_syn_arch. 1 hit.
TIGR00344. AlaS. 1 hit.
PROSITEPS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC9RF77_METVM
AccessionPrimary (citable) accession number: C9RF77
Entry history
Integrated into UniProtKB/TrEMBL: November 24, 2009
Last sequence update: November 24, 2009
Last modified: January 25, 2012
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info