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Protein

Methylated-DNA--protein-cysteine methyltransferase

Gene

ogt

Organism
Methanocaldococcus vulcanius (strain ATCC 700851 / DSM 12094 / M7) (Methanococcus vulcanius)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.UniRule annotation

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

Catalytic activityi

DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei128Nucleophile; methyl group acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processDNA damage, DNA repair

Names & Taxonomyi

Protein namesi
Recommended name:
Methylated-DNA--protein-cysteine methyltransferaseUniRule annotation (EC:2.1.1.63UniRule annotation)
Alternative name(s):
6-O-methylguanine-DNA methyltransferaseUniRule annotation
Short name:
MGMTUniRule annotation
O-6-methylguanine-DNA-alkyltransferaseUniRule annotation
Gene namesi
Name:ogtUniRule annotation
Ordered Locus Names:Metvu_1714
OrganismiMethanocaldococcus vulcanius (strain ATCC 700851 / DSM 12094 / M7) (Methanococcus vulcanius)
Taxonomic identifieri579137 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000002063 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004173551 – 161Methylated-DNA--protein-cysteine methyltransferaseAdd BLAST161

Interactioni

Protein-protein interaction databases

STRINGi579137.Metvu_1714.

Structurei

3D structure databases

ProteinModelPortaliC9RE37.
SMRiC9RE37.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MGMT family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG02724. Archaea.
COG0350. LUCA.
HOGENOMiHOG000244137.
KOiK00567.
OMAiILKLYFA.
OrthoDBiPOG093Z0E1E.

Family and domain databases

CDDicd06445. ATase. 1 hit.
Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00772. OGT. 1 hit.
InterProiView protein in InterPro
IPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR023546. MGMT.
IPR011991. WHTH_DNA-bd_dom.
PfamiView protein in Pfam
PF01035. DNA_binding_1. 1 hit.
SUPFAMiSSF46767. SSF46767. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.
PROSITEiView protein in PROSITE
PS00374. MGMT. 1 hit.

Sequencei

Sequence statusi: Complete.

C9RE37-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVQINDYFI GMIFKGNRLV RNTIPLREEE IFRFYNGKIL DEPNKMCMKV
60 70 80 90 100
GKIILKLYFA DLDDKIARDL IDYELNVSSF TKKVLDEVKK IKFGETVSYG
110 120 130 140 150
DIAKKLNTSP RAVGVALSKN PLPLIIPCHR VVAKNSLGGY SYGIEKKKFI
160
LEMEQIKKRL K
Length:161
Mass (Da):18,548
Last modified:November 24, 2009 - v1
Checksum:i9315A13509221B72
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001787 Genomic DNA. Translation: ACX73566.1.
RefSeqiWP_015733784.1. NC_013407.1.

Genome annotation databases

EnsemblBacteriaiACX73566; ACX73566; Metvu_1714.
GeneIDi8514075.
KEGGimvu:Metvu_1714.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001787 Genomic DNA. Translation: ACX73566.1.
RefSeqiWP_015733784.1. NC_013407.1.

3D structure databases

ProteinModelPortaliC9RE37.
SMRiC9RE37.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi579137.Metvu_1714.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACX73566; ACX73566; Metvu_1714.
GeneIDi8514075.
KEGGimvu:Metvu_1714.

Phylogenomic databases

eggNOGiarCOG02724. Archaea.
COG0350. LUCA.
HOGENOMiHOG000244137.
KOiK00567.
OMAiILKLYFA.
OrthoDBiPOG093Z0E1E.

Family and domain databases

CDDicd06445. ATase. 1 hit.
Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00772. OGT. 1 hit.
InterProiView protein in InterPro
IPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR023546. MGMT.
IPR011991. WHTH_DNA-bd_dom.
PfamiView protein in Pfam
PF01035. DNA_binding_1. 1 hit.
SUPFAMiSSF46767. SSF46767. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.
PROSITEiView protein in PROSITE
PS00374. MGMT. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiOGT_METVM
AccessioniPrimary (citable) accession number: C9RE37
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2012
Last sequence update: November 24, 2009
Last modified: March 15, 2017
This is version 40 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.