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Protein

Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutB

Gene

rutB

Organism
Escherichia coli (strain ATCC 33849 / DSM 4235 / NCIB 12045 / K12 / DH1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby releasing one of the pyrimidine rings nitrogen atoms as ammonia and one of its carbons as CO2.UniRule annotation

Catalytic activityi

(Z)-3-ureidoacrylate peracid + H2O = (Z)-3-peroxyaminoacrylate + CO2 + NH3.UniRule annotation
(Z)-3-ureidoacrylate + H2O = (Z)-3-aminoacrylate + CO2 + NH3.UniRule annotation
(Z)-2-methyl-ureidoacrylate peracid + H2O = (Z)-2-methyl-peroxyaminoacrylate + CO2 + NH3.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei166NucleophileUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutBUniRule annotation (EC:3.5.1.110UniRule annotation)
Alternative name(s):
Ureidoacrylate amidohydrolaseUniRule annotation
Gene namesi
Name:rutBUniRule annotation
Ordered Locus Names:EcDH1_2631, ECDH1ME8569_0965
OrganismiEscherichia coli (strain ATCC 33849 / DSM 4235 / NCIB 12045 / K12 / DH1)
Taxonomic identifieri536056 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004026571 – 230Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutBAdd BLAST230

Expressioni

Inductioni

Up-regulated by the nitrogen regulatory protein C (NtrC also called GlnG) and repressed by RutR.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliC9QZ65
SMRiC9QZ65
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the isochorismatase family. RutB subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000078669
KOiK09020
OMAiWHKSNAL

Family and domain databases

Gene3Di3.40.50.850, 1 hit
HAMAPiMF_00830 RutB, 1 hit
InterProiView protein in InterPro
IPR019916 IsoChorase_RutB
IPR000868 Isochorismatase-like
IPR036380 Isochorismatase-like_sf
PfamiView protein in Pfam
PF00857 Isochorismatase, 1 hit
SUPFAMiSSF52499 SSF52499, 1 hit
TIGRFAMsiTIGR03614 RutB, 1 hit

Sequencei

Sequence statusi: Complete.

C9QZ65-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTLTARPEA ITFDPQQSAL IVVDMQNAYA TPGGYLDLAG FDVSTTRPVI
60 70 80 90 100
ANIQTAVTAA RAAGMLIIWF QNGWDEQYVE AGGPGSPNFH KSNALKTMRK
110 120 130 140 150
QPQLQGKLLA KGSWDYQLVD ELVPQPGDIV LPKPRYSGFF NTPLDSILRS
160 170 180 190 200
RGIRHLVFTG IATNVCVEST LRDGFFLEYF GVVLEDATHQ AGPKFAQKAA
210 220 230
LFNIETFFGW VSDVETFCDA LSPTSFAHIA
Length:230
Mass (Da):25,209
Last modified:June 13, 2012 - v2
Checksum:i6E265222D32A6E80
GO

Sequence cautioni

The sequence ACX40265 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001637 Genomic DNA Translation: ACX40265.1 Different initiation.
AP012030 Genomic DNA Translation: BAJ42821.1
RefSeqiWP_001393558.1, NC_017638.1

Genome annotation databases

EnsemblBacteriaiACX40265; ACX40265; EcDH1_2631
BAJ42821; BAJ42821; ECDH1ME8569_0965
KEGGiedh:EcDH1_2631
edj:ECDH1ME8569_0965
PATRICifig|536056.19.peg.1022

Similar proteinsi

Entry informationi

Entry nameiRUTB_ECOD1
AccessioniPrimary (citable) accession number: C9QZ65
Secondary accession number(s): E6P340
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: June 13, 2012
Last modified: April 25, 2018
This is version 47 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

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