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Protein

Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutB

Gene

rutB

Organism
Escherichia coli (strain ATCC 33849 / DSM 4235 / NCIB 12045 / K12 / DH1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby releasing one of the pyrimidine rings nitrogen atoms as ammonia and one of its carbons as CO2.UniRule annotation

Catalytic activityi

(Z)-3-ureidoacrylate peracid + H2O = (Z)-3-peroxyaminoacrylate + CO2 + NH3.UniRule annotation
(Z)-3-ureidoacrylate + H2O = (Z)-3-aminoacrylate + CO2 + NH3.UniRule annotation
(Z)-2-methyl-ureidoacrylate peracid + H2O = (Z)-2-methyl-peroxyaminoacrylate + CO2 + NH3.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei166 – 1661NucleophileUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutBUniRule annotation (EC:3.5.1.110UniRule annotation)
Alternative name(s):
Ureidoacrylate amidohydrolaseUniRule annotation
Gene namesi
Name:rutBUniRule annotation
Ordered Locus Names:EcDH1_2631, ECDH1ME8569_0965
OrganismiEscherichia coli (strain ATCC 33849 / DSM 4235 / NCIB 12045 / K12 / DH1)
Taxonomic identifieri536056 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 230230Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutBPRO_0000402657Add
BLAST

Expressioni

Inductioni

Up-regulated by the nitrogen regulatory protein C (NtrC also called GlnG) and repressed by RutR.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliC9QZ65.
SMRiC9QZ65. Positions 9-221.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the isochorismatase family. RutB subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000078669.
KOiK09020.
OMAiLAKGGWD.

Family and domain databases

Gene3Di3.40.50.850. 1 hit.
HAMAPiMF_00830. RutB.
InterProiIPR019916. IsoChorase_RutB.
IPR000868. Isochorismatase-like.
[Graphical view]
PfamiPF00857. Isochorismatase. 1 hit.
[Graphical view]
SUPFAMiSSF52499. SSF52499. 1 hit.
TIGRFAMsiTIGR03614. RutB. 1 hit.

Sequencei

Sequence statusi: Complete.

C9QZ65-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTLTARPEA ITFDPQQSAL IVVDMQNAYA TPGGYLDLAG FDVSTTRPVI
60 70 80 90 100
ANIQTAVTAA RAAGMLIIWF QNGWDEQYVE AGGPGSPNFH KSNALKTMRK
110 120 130 140 150
QPQLQGKLLA KGSWDYQLVD ELVPQPGDIV LPKPRYSGFF NTPLDSILRS
160 170 180 190 200
RGIRHLVFTG IATNVCVEST LRDGFFLEYF GVVLEDATHQ AGPKFAQKAA
210 220 230
LFNIETFFGW VSDVETFCDA LSPTSFAHIA
Length:230
Mass (Da):25,209
Last modified:June 13, 2012 - v2
Checksum:i6E265222D32A6E80
GO

Sequence cautioni

The sequence ACX40265.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001637 Genomic DNA. Translation: ACX40265.1. Different initiation.
AP012030 Genomic DNA. Translation: BAJ42821.1.
RefSeqiWP_001393558.1. NC_017638.1.

Genome annotation databases

EnsemblBacteriaiACX40265; ACX40265; EcDH1_2631.
BAJ42821; BAJ42821; ECDH1ME8569_0965.
KEGGiedh:EcDH1_2631.
edj:ECDH1ME8569_0965.
PATRICi36697628. VBIEscCol36761_2778.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001637 Genomic DNA. Translation: ACX40265.1. Different initiation.
AP012030 Genomic DNA. Translation: BAJ42821.1.
RefSeqiWP_001393558.1. NC_017638.1.

3D structure databases

ProteinModelPortaliC9QZ65.
SMRiC9QZ65. Positions 9-221.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACX40265; ACX40265; EcDH1_2631.
BAJ42821; BAJ42821; ECDH1ME8569_0965.
KEGGiedh:EcDH1_2631.
edj:ECDH1ME8569_0965.
PATRICi36697628. VBIEscCol36761_2778.

Phylogenomic databases

HOGENOMiHOG000078669.
KOiK09020.
OMAiLAKGGWD.

Family and domain databases

Gene3Di3.40.50.850. 1 hit.
HAMAPiMF_00830. RutB.
InterProiIPR019916. IsoChorase_RutB.
IPR000868. Isochorismatase-like.
[Graphical view]
PfamiPF00857. Isochorismatase. 1 hit.
[Graphical view]
SUPFAMiSSF52499. SSF52499. 1 hit.
TIGRFAMsiTIGR03614. RutB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33849 / DSM 4235 / NCIB 12045 / K12 / DH1.
  2. "Comparison of sequence reads obtained from three next-generation sequencing platforms."
    Suzuki S., Ono N., Furusawa C., Ying B.W., Yomo T.
    PLoS ONE 6:E19534-E19534(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33849 / DSM 4235 / NCIB 12045 / K12 / DH1.

Entry informationi

Entry nameiRUTB_ECOD1
AccessioniPrimary (citable) accession number: C9QZ65
Secondary accession number(s): E6P340
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: June 13, 2012
Last modified: July 22, 2015
This is version 39 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.