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Protein
Submitted name:

Alpha amylase catalytic region

Gene

amyA

Organism
Escherichia coli (strain ATCC 33849 / DSM 4235 / NCIB 12045 / K12 / DH1)
Status
Unreviewed-Annotation score: Annotation score: 1 out of 5-Protein predictedi

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei235 – 2351NucleophileUniRule annotation
Active sitei265 – 2651Proton donorUniRule annotation

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Submitted name:
Alpha amylase catalytic regionImported
Submitted name:
Cytoplasmic alpha-amylaseImported
Gene namesi
Name:amyAImported
Ordered Locus Names:EcDH1_1719Imported, ECDH1ME8569_1867Imported
OrganismiEscherichia coli (strain ATCC 33849 / DSM 4235 / NCIB 12045 / K12 / DH1)Imported
Taxonomic identifieri536056 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000004998 Componenti: Chromosome UP000002619 Componenti: Chromosome

Structurei

3D structure databases

ProteinModelPortaliC9QSP6.
SMRiC9QSP6. Positions 3-490.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

HOGENOMiHOG000094847.
KOiK01176.
OMAiFFHWYYP.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR013776. A-amylase_thermo.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
[Graphical view]
PIRSFiPIRSF001021. Alph-amls_thrmst. 1 hit.
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

C9QSP6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRNPTLLQCF HWYYPEGGKL WPELAERADG FNDIGINMVW LPPAYKGASG
60 70 80 90 100
GYSVGYDSYD LFDLGEFDQK GSIPTKYGDK AQLLAAIDAL KRNDIAVLLD
110 120 130 140 150
VVVNHKMGAD EKEAIRVQRV NADDRTQIDE EIIECEGWTR YTFPARAGQY
160 170 180 190 200
SQFIWDFKCF SGIDHIENPD EDGIFKIVND YTGEGWNDQV DDELGNFDYL
210 220 230 240 250
MGENIDFRNH AVTEEIKYWA RWVMEQTQCD GFRLDAVKHI PAWFYKEWIE
260 270 280 290 300
HVQEVAPKPL FIVAEYWSHE VDKLQTYIDQ VEGKTMLFDA PLQMKFHEAS
310 320 330 340 350
RMGRDYDMTQ IFTGTLVEAD PFHAVTLVAN HDTQPLQALE APVEPWFKPL
360 370 380 390 400
AYALILLREN GVPSVFYPDL YGAHYEDVGG DGQTYPIDMP IIEQLDELIL
410 420 430 440 450
ARQRFAHGVQ TLFFDHPNCI AFSRSGTDEF PGCVVVMSNG DDGEKTIHLG
460 470 480 490
ENYGNKTWRD FLGNRQERVV TDENGEATFF CNGGSVSVWV IEEVI
Length:495
Mass (Da):56,639
Last modified:November 24, 2009 - v1
Checksum:i26AFF6797DDA54D6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001637 Genomic DNA. Translation: ACX39378.1.
AP012030 Genomic DNA. Translation: BAJ43723.1.
RefSeqiYP_006091667.1. NC_017625.1.
YP_006129268.1. NC_017638.1.

Genome annotation databases

EnsemblBacteriaiACX39378; ACX39378; EcDH1_1719.
BAJ43723; BAJ43723; ECDH1ME8569_1867.
KEGGiedh:EcDH1_1719.
edj:ECDH1ME8569_1867.
PATRICi36695686. VBIEscCol36761_1815.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001637 Genomic DNA. Translation: ACX39378.1.
AP012030 Genomic DNA. Translation: BAJ43723.1.
RefSeqiYP_006091667.1. NC_017625.1.
YP_006129268.1. NC_017638.1.

3D structure databases

ProteinModelPortaliC9QSP6.
SMRiC9QSP6. Positions 3-490.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACX39378; ACX39378; EcDH1_1719.
BAJ43723; BAJ43723; ECDH1ME8569_1867.
KEGGiedh:EcDH1_1719.
edj:ECDH1ME8569_1867.
PATRICi36695686. VBIEscCol36761_1815.

Phylogenomic databases

HOGENOMiHOG000094847.
KOiK01176.
OMAiFFHWYYP.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR013776. A-amylase_thermo.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
[Graphical view]
PIRSFiPIRSF001021. Alph-amls_thrmst. 1 hit.
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DH1Imported.
  2. "Comparison of sequence reads obtained from three next-generation sequencing platforms."
    Suzuki S., Ono N., Furusawa C., Ying B.W., Yomo T.
    PLoS ONE 6:E19534-E19534(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33849 / DSM 4235 / NCIB 12045 / K12 / DH1Imported and DH1Imported.

Entry informationi

Entry nameiC9QSP6_ECOD1
AccessioniPrimary (citable) accession number: C9QSP6
Entry historyi
Integrated into UniProtKB/TrEMBL: November 24, 2009
Last sequence update: November 24, 2009
Last modified: April 29, 2015
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.