ID C9A7W4_ENTCA Unreviewed; 488 AA. AC C9A7W4; DT 03-NOV-2009, integrated into UniProtKB/TrEMBL. DT 03-NOV-2009, sequence version 1. DT 27-MAR-2024, entry version 59. DE RecName: Full=Glycosyl hydrolase family 13 catalytic domain-containing protein {ECO:0000259|SMART:SM00642}; GN ORFNames=ECBG_00844 {ECO:0000313|EMBL:EEV38575.1}; OS Enterococcus casseliflavus EC20. OC Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae; OC Enterococcus. OX NCBI_TaxID=565655 {ECO:0000313|EMBL:EEV38575.1, ECO:0000313|Proteomes:UP000012675}; RN [1] {ECO:0000313|EMBL:EEV38575.1, ECO:0000313|Proteomes:UP000012675} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=EC20 {ECO:0000313|EMBL:EEV38575.1, RC ECO:0000313|Proteomes:UP000012675}; RG The Broad Institute Genome Sequencing Platform; RA Feldgarden M., Young S.K., Kodira C.D., Zeng Q., Koehrsen M., Alvarado L., RA Berlin A., Borenstein D., Chen Z., Engels R., Freedman E., Gellesch M., RA Goldberg J., Griggs A., Gujja S., Heiman D., Hepburn T., Howarth C., RA Jen D., Larson L., Lewis B., Mehta T., Park D., Pearson M., Roberts A., RA Saif S., Shea T., Shenoy N., Sisk P., Stolte C., Sykes S., Walk T., RA White J., Yandava C., Gilmore M., Manson J., Palmer K., Carniol K., RA Lander E., Nusbaum C., Galagan J., Birren B.; RL Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|EMBL:EEV38575.1, ECO:0000313|Proteomes:UP000012675} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=EC20 {ECO:0000313|EMBL:EEV38575.1, RC ECO:0000313|Proteomes:UP000012675}; RG The Broad Institute Genomics Platform; RG The Broad Institute Genome Sequencing Center for Infectious Disease; RA Russ C., Feldgarden M., Gilmore M., Manson J., Palmer K., Carniol K., RA Walker B., Young S.K., Zeng Q., Gargeya S., Fitzgerald M., Haas B., RA Abouelleil A., Allen A.W., Alvarado L., Arachchi H.M., Berlin A.M., RA Chapman S.B., Gainer-Dewar J., Goldberg J., Griggs A., Gujja S., Hansen M., RA Howarth C., Imamovic A., Ireland A., Larimer J., McCowan C., Murphy C., RA Pearson M., Poon T.W., Priest M., Roberts A., Saif S., Shea T., Sisk P., RA Sykes S., Wortman J., Nusbaum C., Birren B.; RT "The Genome Sequence of Enterococcus casseliflavus EC20 (899205)."; RL Submitted (MAR-2013) to the EMBL/GenBank/DDBJ databases. CC -!- COFACTOR: CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108; CC Evidence={ECO:0000256|ARBA:ARBA00001913}; CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. CC {ECO:0000256|ARBA:ARBA00008061, ECO:0000256|RuleBase:RU003615}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP004856; EEV38575.1; -; Genomic_DNA. DR RefSeq; WP_015509620.1; NZ_AKCC01000001.1. DR AlphaFoldDB; C9A7W4; -. DR GeneID; 15142061; -. DR KEGG; ecas:ECBG_00844; -. DR eggNOG; COG0366; Bacteria. DR HOGENOM; CLU_024572_2_0_9; -. DR Proteomes; UP000012675; Chromosome. DR GO; GO:0004556; F:alpha-amylase activity; IEA:InterPro. DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro. DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro. DR CDD; cd11318; AmyAc_bac_fung_AmyA; 1. DR Gene3D; 2.40.30.140; -; 1. DR Gene3D; 3.20.20.80; Glycosidases; 1. DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1. DR InterPro; IPR013776; A-amylase_thermo. DR InterPro; IPR006046; Alpha_amylase. DR InterPro; IPR006047; Glyco_hydro_13_cat_dom. DR InterPro; IPR013780; Glyco_hydro_b. DR InterPro; IPR017853; Glycoside_hydrolase_SF. DR PANTHER; PTHR43447; ALPHA-AMYLASE; 1. DR PANTHER; PTHR43447:SF49; ALPHA-AMYLASE; 1. DR Pfam; PF00128; Alpha-amylase; 1. DR PIRSF; PIRSF001021; Alph-amls_thrmst; 1. DR PRINTS; PR00110; ALPHAAMYLASE. DR SMART; SM00642; Aamy; 1. DR SUPFAM; SSF51445; (Trans)glycosidases; 1. DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1. PE 3: Inferred from homology; KW Calcium {ECO:0000256|PIRSR:PIRSR001021-2}; KW Glycosidase {ECO:0000256|ARBA:ARBA00023295}; KW Hydrolase {ECO:0000256|ARBA:ARBA00022801}; KW Metal-binding {ECO:0000256|PIRSR:PIRSR001021-2}; KW Reference proteome {ECO:0000313|Proteomes:UP000012675}. FT DOMAIN 4..388 FT /note="Glycosyl hydrolase family 13 catalytic" FT /evidence="ECO:0000259|SMART:SM00642" FT ACT_SITE 231 FT /note="Nucleophile" FT /evidence="ECO:0000256|PIRSR:PIRSR001021-1" FT ACT_SITE 261 FT /note="Proton donor" FT /evidence="ECO:0000256|PIRSR:PIRSR001021-1" FT BINDING 104 FT /ligand="Ca(2+)" FT /ligand_id="ChEBI:CHEBI:29108" FT /ligand_label="1" FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2" FT BINDING 194 FT /ligand="Ca(2+)" FT /ligand_id="ChEBI:CHEBI:29108" FT /ligand_label="1" FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2" FT BINDING 200 FT /ligand="Ca(2+)" FT /ligand_id="ChEBI:CHEBI:29108" FT /ligand_label="1" FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2" FT BINDING 202 FT /ligand="Ca(2+)" FT /ligand_id="ChEBI:CHEBI:29108" FT /ligand_label="2" FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2" FT BINDING 235 FT /ligand="Ca(2+)" FT /ligand_id="ChEBI:CHEBI:29108" FT /ligand_label="1" FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2" FT BINDING 300 FT /ligand="Ca(2+)" FT /ligand_id="ChEBI:CHEBI:29108" FT /ligand_label="3" FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2" SQ SEQUENCE 488 AA; 55820 MW; 6147287459C7C9F2 CRC64; MNNKVILQGF EWDLPADSQH WNKLKEMSKE IKEYGFTAIW LPPAYKGTAG SEDVGYGVYD YYDLGEFDQK GSVPTKYGTK DEYLEAIKAL QEQGIEVYAD IVFNHLIGAD EKEVVPAVKY NTENRNEPIS DEEEIEAWTK FTFPGRQGTY NDYVWTWHNF SGVDYDDRNK DHGIFNFADK GWENQVDDEM GNYDYLMGCD LDMENPETVE QLNKWGQWYQ ELTGVDGYRL DAVKHIEFNY YADWILNRRE EKGKPLFVVG EYWSGDLPKL EEYLDSSGNL IYLFDVPLHY NLYEASHSNG EYDMSQIFAG TLTAAREDYA VTFVDNHDTQ VGQSLQSWID GWFKVHAYSL ILLRKAGVPV VFWGDLFGIP AQEIAPVGDA LVHMLKLREK LAFGNQVDYF DDPNCIGWVL TGDYEHELSG LAVVMTNAQG AEKEMTISAI HGGETFVDIL RNNEAKVVLD DTGKGVFPVN DGQVSIYVNE KLVEKYFS //