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Protein

Formate dehydrogenase 1

Gene

FDH1

Organism
Saccharomyces cerevisiae (strain Lalvin EC1118 / Prise de mousse) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the NAD+-dependent oxidation of formate to carbon dioxide. Formate oxidation is the final step in the methanol oxidation pathway in methylotrophic microorganisms. Has a role in the detoxification of exogenous formate in non-methylotrophic organisms.UniRule annotation

Catalytic activityi

Formate + NAD+ = CO2 + NADH.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei97 – 971Substrate; via amide nitrogenUniRule annotation
Binding sitei121 – 1211SubstrateUniRule annotation
Binding sitei197 – 1971NADUniRule annotation
Binding sitei270 – 2701NAD; via carbonyl oxygenUniRule annotation
Sitei272 – 2721Important for catalytic activityUniRule annotation
Binding sitei296 – 2961NADUniRule annotation
Sitei325 – 3251Important for catalytic activityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi176 – 1772NADUniRule annotation
Nucleotide bindingi244 – 2485NADUniRule annotation
Nucleotide bindingi325 – 3284NADUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Formate dehydrogenase 1UniRule annotation (EC:1.2.1.2UniRule annotation)
Short name:
FDHUniRule annotation
Alternative name(s):
NAD-dependent formate dehydrogenaseUniRule annotation
Gene namesi
Name:FDH1
ORF Names:EC1118_1O4_6403g
OrganismiSaccharomyces cerevisiae (strain Lalvin EC1118 / Prise de mousse) (Baker's yeast)
Taxonomic identifieri643680 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000000286 Componenti: Chromosome XV, Scaffold EC1118_1O4

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 376376Formate dehydrogenase 1PRO_0000393442Add
BLAST

Expressioni

Inductioni

Induced by formate.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliC8ZHD6.
SMRiC8ZHD6. Positions 4-367.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni5 – 121117CatalyticUniRule annotationAdd
BLAST
Regioni122 – 326205Coenzyme-bindingUniRule annotationAdd
BLAST
Regioni327 – 37246CatalyticUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. FDH subfamily.UniRule annotation

Phylogenomic databases

OrthoDBiEOG769ZV3.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
HAMAPiMF_03210. Formate_dehydrogenase.
InterProiIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR029753. D-isomer_DH_CS.
IPR006140. D-isomer_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00670. D_2_HYDROXYACID_DH_2. 1 hit.
PS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C8ZHD6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKGKVLLVL YEGGKHAEEQ EKLLGCIENE LGIRNFIEEQ GYELVTTIDK
60 70 80 90 100
DPEPTSTVDR ELKDAEIVIT TPFFPAYISR NRIAEAPNLK LCVTAGVGSD
110 120 130 140 150
HVDLEAANER KITVTEVTGS NVVSVAEHVM ATILVLIRNY NGGHQQAING
160 170 180 190 200
EWDIAGVAKN EYDLEDKIIS TVGAGRIGYR VLERLVAFNP KKLLYYDYQE
210 220 230 240 250
LPAEAINRLN EASKLFNGRG DIVQRVEKLE DMVAQSDVVT INCPLHKDSR
260 270 280 290 300
GLFNKKLISH MKDGAYLVNT ARGAICVAED VAEAVKSGKL AGYGGDVWDK
310 320 330 340 350
QPAPKDHPWR TMDNKDHVGN AMTVHISGTS LDAQKRYAQG VKNILNSYFS
360 370
KKFDYRPQDI IVQNGSYATR AYGQKK
Length:376
Mass (Da):41,714
Last modified:November 3, 2009 - v1
Checksum:i67ECDA6F9DDC2A02
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN394216 Genomic DNA. Translation: CAY86665.1.

Genome annotation databases

EnsemblFungiiCAY86665; CAY86665; EC1118_1O4_6403g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN394216 Genomic DNA. Translation: CAY86665.1.

3D structure databases

ProteinModelPortaliC8ZHD6.
SMRiC8ZHD6. Positions 4-367.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAY86665; CAY86665; EC1118_1O4_6403g.

Phylogenomic databases

OrthoDBiEOG769ZV3.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
HAMAPiMF_03210. Formate_dehydrogenase.
InterProiIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR029753. D-isomer_DH_CS.
IPR006140. D-isomer_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00670. D_2_HYDROXYACID_DH_2. 1 hit.
PS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Eukaryote-to-eukaryote gene transfer events revealed by the genome sequence of the wine yeast Saccharomyces cerevisiae EC1118."
    Novo M., Bigey F., Beyne E., Galeote V., Gavory F., Mallet S., Cambon B., Legras J.-L., Wincker P., Casaregola S., Dequin S.
    Proc. Natl. Acad. Sci. U.S.A. 106:16333-16338(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Lalvin EC1118 / Prise de mousse.

Entry informationi

Entry nameiFDH1_YEAS8
AccessioniPrimary (citable) accession number: C8ZHD6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: November 3, 2009
Last modified: January 20, 2016
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.