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C8X4E2

- C8X4E2_DESRD

UniProt

C8X4E2 - C8X4E2_DESRD

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Protein

Acetyl-coenzyme A synthetase

Gene

acsA

Organism
Desulfohalobium retbaense (strain DSM 5692)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.UniRule annotation

Catalytic activityi

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

Cofactori

Note: Magnesium.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei322 – 3221Coenzyme AUniRule annotation
Binding sitei511 – 5111ATPUniRule annotation
Binding sitei526 – 5261ATPUniRule annotation
Binding sitei534 – 5341Coenzyme A; via carbonyl oxygenUniRule annotation
Binding sitei537 – 5371ATPUniRule annotation
Metal bindingi548 – 5481Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi550 – 5501Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi553 – 5531Magnesium; via carbonyl oxygenUniRule annotation
Binding sitei595 – 5951Coenzyme AUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi398 – 4003ATPUniRule annotation
Nucleotide bindingi422 – 4276ATPUniRule annotation

GO - Molecular functioni

  1. acetate-CoA ligase activity Source: UniProtKB-HAMAP
  2. AMP binding Source: InterPro
  3. ATP binding Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. acetyl-CoA biosynthetic process from acetate Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciDRET485915:GHRJ-2180-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
Short name:
AcCoA synthetaseUniRule annotation
Short name:
AcsUniRule annotation
Alternative name(s):
Acetate--CoA ligaseUniRule annotation
Acyl-activating enzymeUniRule annotation
Gene namesi
Name:acsAUniRule annotation
Ordered Locus Names:Dret_2132Imported
OrganismiDesulfohalobium retbaense (strain DSM 5692)Imported
Taxonomic identifieri485915 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfohalobiaceaeDesulfohalobium
ProteomesiUP000001052: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei620 – 6201N6-acetyllysineUniRule annotation

Post-translational modificationi

Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.UniRule annotation

Keywords - PTMi

AcetylationUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi485915.Dret_2132.

Structurei

3D structure databases

ProteinModelPortaliC8X4E2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni203 – 2064Coenzyme A bindingUniRule annotation

Sequence similaritiesi

Belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0365.
HOGENOMiHOG000229981.
KOiK01895.
OMAiAWIWYRD.
OrthoDBiEOG68WR2H.

Family and domain databases

HAMAPiMF_01123. Ac_CoA_synth.
InterProiIPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C8X4E2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGGSDEHIES MMHERRHFPP PRHGSERAHI PGPEAYDDLR DQAARDPEGF
60 70 80 90 100
WAERAKALLD WDSEWDTVLE ADWETPEFKW FAGGKLNACA NCLDRHVASG
110 120 130 140 150
RGEKTALIWQ GEPEDDVRQF TYAELRDEVV KFAKVLKKWG VGKGDRVAVY
160 170 180 190 200
LPMIPELPIA MLACARIGAV HSVVFAGFSA HSLQNRIQDC EAKVLITADA
210 220 230 240 250
VIRAGKQIPL KTNGDEALEA CPSVEHCIVV NRAGLDVAMH EGRDVWWHDA
260 270 280 290 300
VAGDDGTDCP PESMDAEDLL FILYTSGSTG KPKGVVHTTG GYLTYTAQTT
310 320 330 340 350
QWVFDIQEDD VYWCTADIGW ITGHSYILYG PLALGATTVM FEGVPSYPQP
360 370 380 390 400
DRFWQVVEKF KVTIFYTAPT VIRALMREGP QWTETRDLSS LRLLGSVGEP
410 420 430 440 450
INPEAWMWYH DHVGKGELPV LDTWWQTETG GIMISAVPYA TTLKPGSASQ
460 470 480 490 500
PLPGIEADIV DRDGEPVGPN RGGHLVVKTP WPGMLRNVYG DPARYKSTYF
510 520 530 540 550
ERFPGMYESG DSARMDEEGY FWIMGRLDDV INVSGHRLGT AEIESALVAH
560 570 580 590 600
EAVAEAAVVG MPHEVKGQAI YAYVTLKSTF QENDELRKVL RQHVRTEIGA
610 620 630 640 650
IATPEIIQFA PGLPKTRSGK IMRRILRKIA ADETNAFGDT STLADPTVIA
660
DLIDGKNLLG K
Length:661
Mass (Da):73,245
Last modified:November 3, 2009 - v1
Checksum:i0B1F90A93F31B941
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001734 Genomic DNA. Translation: ACV69416.1.
RefSeqiYP_003198994.1. NC_013223.1.

Genome annotation databases

EnsemblBacteriaiACV69416; ACV69416; Dret_2132.
GeneIDi8419982.
KEGGidrt:Dret_2132.
PATRICi21701382. VBIDesRet71890_2248.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001734 Genomic DNA. Translation: ACV69416.1 .
RefSeqi YP_003198994.1. NC_013223.1.

3D structure databases

ProteinModelPortali C8X4E2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 485915.Dret_2132.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACV69416 ; ACV69416 ; Dret_2132 .
GeneIDi 8419982.
KEGGi drt:Dret_2132.
PATRICi 21701382. VBIDesRet71890_2248.

Phylogenomic databases

eggNOGi COG0365.
HOGENOMi HOG000229981.
KOi K01895.
OMAi AWIWYRD.
OrthoDBi EOG68WR2H.

Enzyme and pathway databases

BioCyci DRET485915:GHRJ-2180-MONOMER.

Family and domain databases

HAMAPi MF_01123. Ac_CoA_synth.
InterProi IPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 5692Imported.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 5692Imported.

Entry informationi

Entry nameiC8X4E2_DESRD
AccessioniPrimary (citable) accession number: C8X4E2
Entry historyi
Integrated into UniProtKB/TrEMBL: November 3, 2009
Last sequence update: November 3, 2009
Last modified: November 26, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3