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Protein

Beta-galactosidase BglY

Gene

bglY

Organism
Alicyclobacillus acidocaldarius subsp. acidocaldarius (strain ATCC 27009 / DSM 446 / JCM 5260 / NBRC 15652 / NCIMB 11725 / NRRL B-14509 / 104-1A) (Bacillus acidocaldarius)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes o-nitrophenyl-beta-D-galactopyranoside (ONPG) and p-nitrophenyl-beta-D-fucopyranoside (PNPF), but not p-nitrophenyl-beta-D-glucopyranoside (PNPG), p-nitrophenyl-beta-D-xylopyranoside (PNPX) or p-nitrophenyl-beta-D-arabinopyranoside (PNPA). Also hydrolyzes lactose, including lactose in milk.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication

Enzyme regulationi

Ca2+, Mg2+ and EDTA have little effect on enzyme activity at 1-10 mM. Zn2+ at 3, 5, 7 or 10 mM inhibits activity by 20%, 30%, 40% and 65%, respectively.1 Publication

Kineticsi

  1. KM=6 mM for ONPG (at 60 degrees Celsius and pH 6.0)1 Publication
  2. KM=3.5 mM for PNPF (at 60 degrees Celsius and pH 6.0)1 Publication

    pH dependencei

    Optimum pH is 5.8. Approximately 80% of activity retained after incubating the enzyme for 40 minutes in buffers ranging from pH 5.0 to pH 10.5.1 Publication

    Temperature dependencei

    Optimum temperature is 70 degrees Celsius. Retains 90% of activity when heated at 70 degrees Celsius for 30 minutes. Approximately 48% of lactose in milk is hydrolyzed following treatment with enzyme at 65 degrees Celsius over 60 minutes.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei118 – 1181SubstrateBy similarity
    Metal bindingi122 – 1221ZincBy similarity
    Binding sitei156 – 1561SubstrateBy similarity
    Active sitei157 – 1571Proton donorBy similarity
    Metal bindingi162 – 1621ZincBy similarity
    Metal bindingi164 – 1641ZincBy similarity
    Metal bindingi167 – 1671ZincBy similarity
    Active sitei313 – 3131NucleophileBy similarity
    Binding sitei321 – 3211SubstrateBy similarity

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciAACI521098:GCIO-2974-MONOMER.

    Protein family/group databases

    CAZyiGH42. Glycoside Hydrolase Family 42.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidase BglY (EC:3.2.1.23)
    Short name:
    Beta-galBy similarity
    Gene namesi
    Name:bglY
    Ordered Locus Names:Aaci_2891
    OrganismiAlicyclobacillus acidocaldarius subsp. acidocaldarius (strain ATCC 27009 / DSM 446 / JCM 5260 / NBRC 15652 / NCIMB 11725 / NRRL B-14509 / 104-1A) (Bacillus acidocaldarius)
    Taxonomic identifieri521098 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesAlicyclobacillaceaeAlicyclobacillus
    ProteomesiUP000001917 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Pathology & Biotechi

    Biotechnological usei

    Has potential use in hydrolyzing lactose in neutral pH dairy products such as whole milk or whey. Also could be used in milk lactose hydrolysis during pasteurization at high temperatures.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 688688Beta-galactosidase BglYPRO_0000407679Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi521098.Aaci_2891.

    Structurei

    3D structure databases

    ProteinModelPortaliC8WV58.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni361 – 3644Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 42 family.Sequence Analysis

    Phylogenomic databases

    eggNOGiCOG1874.
    HOGENOMiHOG000117811.
    KOiK12308.
    OMAiMWHRDKD.
    OrthoDBiEOG6GTZGG.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    3.40.50.880. 1 hit.
    InterProiIPR013739. Beta_galactosidase_C.
    IPR013738. Beta_galactosidase_Trimer.
    IPR029062. Class_I_gatase-like.
    IPR003476. Glyco_hydro_42.
    IPR013529. Glyco_hydro_42_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02449. Glyco_hydro_42. 1 hit.
    PF08533. Glyco_hydro_42C. 1 hit.
    PF08532. Glyco_hydro_42M. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001084. B-galactosidase. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF52317. SSF52317. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    C8WV58-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAKHAPIFPN VQGFLHGGDY NPDQWLAYPD VLEQDVQLMR EAKWNVVSLG
    60 70 80 90 100
    IFSWVSLEPE EGLFTFEWLD EAIERLTHAG VRILLATPSG ARPAWLSAKY
    110 120 130 140 150
    PEVLRVGPDG RRNRHGGRHN HCYTSPIYRE KVRIINRKLA ERYAHHPGVI
    160 170 180 190 200
    GWHVSNEYGG ECHCPLCQEA FREWLKRKYK TLDALNHAWW TPFWSHTYTD
    210 220 230 240 250
    WSQIESPMPH GETSIHGLNL DWKRFVTDQT VDFCRHEIEP LKQVNPNLPV
    260 270 280 290 300
    TTNFMGTYPG LNYWRFRDVL DVISWDSYPR WHAHETLVPE AVHTAMVHDL
    310 320 330 340 350
    NRSILKKPFL LMESTPSVTN WQAVSKQKRP GVHVLVSLQA VAHGADSVQY
    360 370 380 390 400
    FQWRKSRGSY EKFHGAVVDH VGHANTRVFR DVQAVGEMLE RLAPMAGAEV
    410 420 430 440 450
    KADAAVIFDW ENRWALEDAK GPRNIGMHYE ETVVNHYAAL WRMGVPMDVI
    460 470 480 490 500
    DEEQPLDGYK LVVAPMLYMV RPGVAERMKA FVERGGSLVL TYWSGIVDEN
    510 520 530 540 550
    DLVFLGGFPG PLRELAGVWA EEIDALYDGE RVPVRVADGN PLGLAGHYEA
    560 570 580 590 600
    RELCEVVHLE GAEPIAVYGA DYYEGMPAAT VHRVGKGKVY YVAARLEDAF
    610 620 630 640 650
    LRDFFARVAA EAGVARAIER ELPDGVSAMV RSGDGVEYVM LMNFTPEARE
    660 670 680
    VALDEAEYKP LYGEAPTDGA VRLPAYGVSV LERPARNG
    Length:688
    Mass (Da):77,869
    Last modified:November 3, 2009 - v1
    Checksum:i686AFEA665822767
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti543 – 5442Missing AA sequence (PubMed:17914606).Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    DQ887754 Genomic DNA. Translation: ABI84370.1.
    CP001727 Genomic DNA. Translation: ACV59895.1.
    RefSeqiWP_012812096.1. NC_013205.1.
    YP_003186284.1. NC_013205.1.

    Genome annotation databases

    EnsemblBacteriaiACV59895; ACV59895; Aaci_2891.
    KEGGiaac:Aaci_2891.
    PATRICi20849346. VBIAliAci73240_2885.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    DQ887754 Genomic DNA. Translation: ABI84370.1.
    CP001727 Genomic DNA. Translation: ACV59895.1.
    RefSeqiWP_012812096.1. NC_013205.1.
    YP_003186284.1. NC_013205.1.

    3D structure databases

    ProteinModelPortaliC8WV58.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi521098.Aaci_2891.

    Protein family/group databases

    CAZyiGH42. Glycoside Hydrolase Family 42.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiACV59895; ACV59895; Aaci_2891.
    KEGGiaac:Aaci_2891.
    PATRICi20849346. VBIAliAci73240_2885.

    Phylogenomic databases

    eggNOGiCOG1874.
    HOGENOMiHOG000117811.
    KOiK12308.
    OMAiMWHRDKD.
    OrthoDBiEOG6GTZGG.

    Enzyme and pathway databases

    BioCyciAACI521098:GCIO-2974-MONOMER.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    3.40.50.880. 1 hit.
    InterProiIPR013739. Beta_galactosidase_C.
    IPR013738. Beta_galactosidase_Trimer.
    IPR029062. Class_I_gatase-like.
    IPR003476. Glyco_hydro_42.
    IPR013529. Glyco_hydro_42_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02449. Glyco_hydro_42. 1 hit.
    PF08533. Glyco_hydro_42C. 1 hit.
    PF08532. Glyco_hydro_42M. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001084. B-galactosidase. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF52317. SSF52317. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Heterologous expression of a gene encoding a thermostable beta-galactosidase from Alicyclobacillus acidocaldarius."
      Yuan T., Yang P., Wang Y., Meng K., Luo H., Zhang W., Wu N., Fan Y., Yao B.
      Biotechnol. Lett. 30:343-348(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-8; 536-551 AND 637-649, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, BIOTECHNOLOGY.
      Strain: ATCC 27009 / DSM 446 / JCM 5260 / NBRC 15652 / NCIMB 11725 / NRRL B-14509 / 104-1AImported.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 27009 / DSM 446 / JCM 5260 / NBRC 15652 / NCIMB 11725 / NRRL B-14509 / 104-1AImported.

    Entry informationi

    Entry nameiBGAL_ALIAD
    AccessioniPrimary (citable) accession number: C8WV58
    Secondary accession number(s): Q06GJ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 3, 2011
    Last sequence update: November 3, 2009
    Last modified: April 29, 2015
    This is version 33 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.