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C8WV58

- BGAL_ALIAD

UniProt

C8WV58 - BGAL_ALIAD

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Protein

Beta-galactosidase BglY

Gene
bglY, Aaci_2891
Organism
Alicyclobacillus acidocaldarius subsp. acidocaldarius (strain ATCC 27009 / DSM 446 / 104-1A) (Bacillus acidocaldarius)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes o-nitrophenyl-beta-D-galactopyranoside (ONPG) and p-nitrophenyl-beta-D-fucopyranoside (PNPF), but not p-nitrophenyl-beta-D-glucopyranoside (PNPG), p-nitrophenyl-beta-D-xylopyranoside (PNPX) or p-nitrophenyl-beta-D-arabinopyranoside (PNPA). Also hydrolyzes lactose, including lactose in milk.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication

Enzyme regulationi

Ca2+, Mg2+ and EDTA have little effect on enzyme activity at 1-10 mM. Zn2+ at 3, 5, 7 or 10 mM inhibits activity by 20%, 30%, 40% and 65%, respectively.1 Publication

Kineticsi

  1. KM=6 mM for ONPG (at 60 degrees Celsius and pH 6.0)1 Publication
  2. KM=3.5 mM for PNPF (at 60 degrees Celsius and pH 6.0)

pH dependencei

Optimum pH is 5.8. Approximately 80% of activity retained after incubating the enzyme for 40 minutes in buffers ranging from pH 5.0 to pH 10.5.

Temperature dependencei

Optimum temperature is 70 degrees Celsius. Retains 90% of activity when heated at 70 degrees Celsius for 30 minutes. Approximately 48% of lactose in milk is hydrolyzed following treatment with enzyme at 65 degrees Celsius over 60 minutes.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181Substrate By similarity
Metal bindingi122 – 1221Zinc By similarity
Binding sitei156 – 1561Substrate By similarity
Active sitei157 – 1571Proton donor By similarity
Metal bindingi162 – 1621Zinc By similarity
Metal bindingi164 – 1641Zinc By similarity
Metal bindingi167 – 1671Zinc By similarity
Active sitei313 – 3131Nucleophile By similarity
Binding sitei321 – 3211Substrate By similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. galactose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciAACI521098:GCIO-2974-MONOMER.

Protein family/group databases

CAZyiGH42. Glycoside Hydrolase Family 42.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase BglY (EC:3.2.1.23)
Short name:
Beta-gal
Gene namesi
Name:bglY
Ordered Locus Names:Aaci_2891
OrganismiAlicyclobacillus acidocaldarius subsp. acidocaldarius (strain ATCC 27009 / DSM 446 / 104-1A) (Bacillus acidocaldarius)
Taxonomic identifieri521098 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesAlicyclobacillaceaeAlicyclobacillus
ProteomesiUP000001917: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

Pathology & Biotechi

Biotechnological usei

Has potential use in hydrolyzing lactose in neutral pH dairy products such as whole milk or whey. Also could be used in milk lactose hydrolysis during pasteurization at high temperatures.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 688688Beta-galactosidase BglYPRO_0000407679Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi521098.Aaci_2891.

Structurei

3D structure databases

ProteinModelPortaliC8WV58.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni361 – 3644Substrate binding

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000117811.
KOiK12308.
OMAiCFLGGFP.
OrthoDBiEOG6GTZGG.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFiPIRSF001084. B-galactosidase. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.

Sequencei

Sequence statusi: Complete.

C8WV58-1 [UniParc]FASTAAdd to Basket

« Hide

MAKHAPIFPN VQGFLHGGDY NPDQWLAYPD VLEQDVQLMR EAKWNVVSLG    50
IFSWVSLEPE EGLFTFEWLD EAIERLTHAG VRILLATPSG ARPAWLSAKY 100
PEVLRVGPDG RRNRHGGRHN HCYTSPIYRE KVRIINRKLA ERYAHHPGVI 150
GWHVSNEYGG ECHCPLCQEA FREWLKRKYK TLDALNHAWW TPFWSHTYTD 200
WSQIESPMPH GETSIHGLNL DWKRFVTDQT VDFCRHEIEP LKQVNPNLPV 250
TTNFMGTYPG LNYWRFRDVL DVISWDSYPR WHAHETLVPE AVHTAMVHDL 300
NRSILKKPFL LMESTPSVTN WQAVSKQKRP GVHVLVSLQA VAHGADSVQY 350
FQWRKSRGSY EKFHGAVVDH VGHANTRVFR DVQAVGEMLE RLAPMAGAEV 400
KADAAVIFDW ENRWALEDAK GPRNIGMHYE ETVVNHYAAL WRMGVPMDVI 450
DEEQPLDGYK LVVAPMLYMV RPGVAERMKA FVERGGSLVL TYWSGIVDEN 500
DLVFLGGFPG PLRELAGVWA EEIDALYDGE RVPVRVADGN PLGLAGHYEA 550
RELCEVVHLE GAEPIAVYGA DYYEGMPAAT VHRVGKGKVY YVAARLEDAF 600
LRDFFARVAA EAGVARAIER ELPDGVSAMV RSGDGVEYVM LMNFTPEARE 650
VALDEAEYKP LYGEAPTDGA VRLPAYGVSV LERPARNG 688
Length:688
Mass (Da):77,869
Last modified:November 3, 2009 - v1
Checksum:i686AFEA665822767
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti543 – 5442Missing AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ887754 Genomic DNA. Translation: ABI84370.1.
CP001727 Genomic DNA. Translation: ACV59895.1.
RefSeqiWP_012812096.1. NC_013205.1.
YP_003186284.1. NC_013205.1.

Genome annotation databases

EnsemblBacteriaiACV59895; ACV59895; Aaci_2891.
GeneIDi8426432.
KEGGiaac:Aaci_2891.
PATRICi20849346. VBIAliAci73240_2885.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ887754 Genomic DNA. Translation: ABI84370.1 .
CP001727 Genomic DNA. Translation: ACV59895.1 .
RefSeqi WP_012812096.1. NC_013205.1.
YP_003186284.1. NC_013205.1.

3D structure databases

ProteinModelPortali C8WV58.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 521098.Aaci_2891.

Protein family/group databases

CAZyi GH42. Glycoside Hydrolase Family 42.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACV59895 ; ACV59895 ; Aaci_2891 .
GeneIDi 8426432.
KEGGi aac:Aaci_2891.
PATRICi 20849346. VBIAliAci73240_2885.

Phylogenomic databases

eggNOGi COG1874.
HOGENOMi HOG000117811.
KOi K12308.
OMAi CFLGGFP.
OrthoDBi EOG6GTZGG.

Enzyme and pathway databases

BioCyci AACI521098:GCIO-2974-MONOMER.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProi IPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view ]
PIRSFi PIRSF001084. B-galactosidase. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Heterologous expression of a gene encoding a thermostable beta-galactosidase from Alicyclobacillus acidocaldarius."
    Yuan T., Yang P., Wang Y., Meng K., Luo H., Zhang W., Wu N., Fan Y., Yao B.
    Biotechnol. Lett. 30:343-348(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-8; 536-551 AND 637-649, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, BIOTECHNOLOGY.
    Strain: ATCC 27009 / DSM 446 / 104-1A.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27009 / DSM 446 / 104-1A.

Entry informationi

Entry nameiBGAL_ALIAD
AccessioniPrimary (citable) accession number: C8WV58
Secondary accession number(s): Q06GJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: November 3, 2009
Last modified: September 3, 2014
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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