C8WTZ7 (C8WTZ7_ALIAD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108
EC=2.7.7.60 HAMAP MF_00108

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase HAMAP MF_00108
MEP cytidylyltransferase HAMAP MF_00108

Gene names

Name:	ispD HAMAP MF_00108
Ordered Locus Names:	Aaci_2735

Organism

Alicyclobacillus acidocaldarius subsp. acidocaldarius (strain ATCC 27009 / DSM 446 / 104-1A) (Bacillus acidocaldarius) [Complete proteome] [HAMAP]

Taxonomic identifier

521098 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Alicyclobacillaceae › Alicyclobacillus

Protein attributes

Sequence length	242 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family. HAMAP MF_00108

Ontologies

Keywords
Biological process	Isoprene biosynthesis HAMAP MF_00108
Molecular function	Nucleotidyltransferase HAMAP MF_00108 SAAS SAAS001228 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Site	14	1	Transition state stabilizer By similarity HAMAP MF_00108
	Site	19	1	Transition state stabilizer By similarity HAMAP MF_00108
	Site	156	1	Positions MEP for the nucleophilic attack By similarity HAMAP MF_00108
	Site	213	1	Positions MEP for the nucleophilic attack By similarity HAMAP MF_00108

Sequences

	Sequence		Length	Mass (Da)
	C8WTZ7 [UniParc]. Last modified November 3, 2009. Version 1. Checksum: C0D9BFA8E8786C04	FASTA	242	26,271
	10 20 30 40 50 60 MWVAIVVAAG EGRRMGFKKQ FLDLAGRPMW LRSVEAMIGG GANEIVVVAS PADVDRMREE 70 80 90 100 110 120 ADGAGRDIHF AEGGETRHAS VVSGVKRALE LVSRQKVDPA RSAFAIHDAA RPFVAEEDVR 130 140 150 160 170 180 RVFEAAVRAG GAVLGSPCRD TVKRVEGGLV QETVPRDKLF LAETPQAIRA DLVPRVYLER 190 200 210 220 230 240 HYSAENSPTD DSGAMEAVGV PVVAVASTAF NGKITTPADL DYARWLARER WGRAEHADRT RV

References

[1]

"The complete chromosome of Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kyrpides N., Mavromatis K., Ivanova N., Ovchinnikova G., Chertkov O., Sims D., Brettin T., Detter J.C., Han C. , Larimer F., Land M., Hauser L., Markowitz V., Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Pukall R., Klenk H.-P., Eisen J.A. Submitted (SEP-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27009 / DSM 446 / 104-1A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001727 Genomic DNA. Translation: ACV59739.1.
RefSeq	YP_003186128.1. NC_013205.1.
3D structure databases
ProteinModelPortal	C8WTZ7.
ModBase	Search...
Protein-protein interaction databases
STRING	C8WTZ7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	8426276.
GenomeReviews	Gene locus Aaci_2735 in contig CP001727_GR.
KEGG	aac:Aaci_2735.
PATRIC	20849012. VBIAliAci73240_2718.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	SKVIVVC.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C8WTZ7_ALIAD

Accession

Primary (citable) accession number: C8WTZ7

Entry history

Integrated into UniProtKB/TrEMBL:	November 3, 2009
Last sequence update:	November 3, 2009
Last modified:	December 14, 2011
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)