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Protein

Probable tRNA sulfurtransferase

Gene

thiI

Organism
Eggerthella lenta (strain ATCC 25559 / DSM 2243 / JCM 9979 / NCTC 11813 / VPI 0255) (Eubacterium lentum)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.UniRule annotationSAAS annotation

Catalytic activityi

L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide.UniRule annotationSAAS annotation
[IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotationSAAS annotation

Pathway: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Pathway: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei270 – 2701ATPUniRule annotation
Binding sitei292 – 2921ATP; via amide nitrogenUniRule annotation
Binding sitei301 – 3011ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi188 – 1892ATPUniRule annotation
Nucleotide bindingi213 – 2142ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

TransferaseUniRule annotationSAAS annotation

Keywords - Biological processi

Thiamine biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding, RNA-binding, tRNA-bindingUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciELEN479437:GHWY-1682-MONOMER.
UniPathwayiUPA00060.
UPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA sulfurtransferaseUniRule annotationSAAS annotation (EC:2.8.1.4UniRule annotationSAAS annotation)
Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferaseUniRule annotation
Thiamine biosynthesis protein ThiIUniRule annotation
tRNA 4-thiouridine synthaseUniRule annotation
Gene namesi
Name:thiIUniRule annotation
Ordered Locus Names:Elen_1658Imported
OrganismiEggerthella lenta (strain ATCC 25559 / DSM 2243 / JCM 9979 / NCTC 11813 / VPI 0255) (Eubacterium lentum)Imported
Taxonomic identifieri479437 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCoriobacteriiaEggerthellalesEggerthellaceaeEggerthella
ProteomesiUP000001377 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Protein-protein interaction databases

STRINGi479437.Elen_1658.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini66 – 170105THUMPUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ThiI family.UniRule annotation
Contains 1 THUMP domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0301.
HOGENOMiHOG000227470.
KOiK03151.
OMAiPDEDCCT.
OrthoDBiEOG6TBHGR.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00021. ThiI.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR020536. ThiI_AANH.
IPR004114. THUMP.
IPR003720. tRNA_STrfase.
[Graphical view]
PfamiPF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view]
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00342. TIGR00342. 1 hit.
PROSITEiPS51165. THUMP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C8WHN5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEFQRICLV HYHEIGLKGH NRSTFEMRLL KNLEALLKPF PVVVIHRIAG
60 70 80 90 100
RLCVFLREGT DWTTAKEAAD VIGKVPGVAR VSCGFKCERD LDEMTEAALA
110 120 130 140 150
AMAEAGEFDT FKVAARRNHT DFATGSMDMN QIIGSALCAA HPEKSVKMKK
160 170 180 190 200
PDVTVGVEVV QNAAYVYARS LPGVGGLPVG SSGLVVSLLS SGIDSPVATW
210 220 230 240 250
KLARRGAVCI GVHFSGRPQT SDASEYLVDD IAQVLERTGC IARVYAVPFG
260 270 280 290 300
DYQREIALTV PPELRVIMYR RLMFKVAEEI ARRERAGALV TGESLGQVAS
310 320 330 340 350
QTLDNIRCTD AAVDLPVFRP LIGTDKLEII AEAERLGSFE ISSQDAPDCC
360 370 380 390 400
TLFMPRSPET HAKLPVVLEA EAALPIERWV PEIADAAEVR DYACPAYKPK

KKRAS
Length:405
Mass (Da):44,215
Last modified:November 3, 2009 - v1
Checksum:iBAFEA9993BFC7547
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001726 Genomic DNA. Translation: ACV55626.1.
RefSeqiWP_015760700.1. NC_013204.1.
YP_003182015.1. NC_013204.1.

Genome annotation databases

EnsemblBacteriaiACV55626; ACV55626; Elen_1658.
KEGGiele:Elen_1658.
PATRICi21844015. VBIEggLen106823_1713.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001726 Genomic DNA. Translation: ACV55626.1.
RefSeqiWP_015760700.1. NC_013204.1.
YP_003182015.1. NC_013204.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi479437.Elen_1658.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACV55626; ACV55626; Elen_1658.
KEGGiele:Elen_1658.
PATRICi21844015. VBIEggLen106823_1713.

Phylogenomic databases

eggNOGiCOG0301.
HOGENOMiHOG000227470.
KOiK03151.
OMAiPDEDCCT.
OrthoDBiEOG6TBHGR.

Enzyme and pathway databases

UniPathwayiUPA00060.
UPA00060.
BioCyciELEN479437:GHWY-1682-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00021. ThiI.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR020536. ThiI_AANH.
IPR004114. THUMP.
IPR003720. tRNA_STrfase.
[Graphical view]
PfamiPF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view]
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00342. TIGR00342. 1 hit.
PROSITEiPS51165. THUMP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25559 / DSM 2243 / JCM 9979 / NCTC 11813 / VPI 0255Imported.

Entry informationi

Entry nameiC8WHN5_EGGLE
AccessioniPrimary (citable) accession number: C8WHN5
Entry historyi
Integrated into UniProtKB/TrEMBL: November 3, 2009
Last sequence update: November 3, 2009
Last modified: June 24, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.