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C8WHN5 (C8WHN5_EGGLE) Unreviewed, UniProtKB/TrEMBL

Last modified July 9, 2014. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable tRNA sulfurtransferase HAMAP-Rule MF_00021

EC=2.8.1.4 HAMAP-Rule MF_00021
Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferase HAMAP-Rule MF_00021
Thiamine biosynthesis protein ThiI HAMAP-Rule MF_00021
tRNA 4-thiouridine synthase HAMAP-Rule MF_00021
Gene names
Name:thiI HAMAP-Rule MF_00021
Ordered Locus Names:Elen_1658 EMBL ACV55626.1
OrganismEggerthella lenta (strain ATCC 25559 / DSM 2243 / JCM 9979 / NCTC 11813 / VPI 0255) (Eubacterium lentum) [Complete proteome] [HAMAP] EMBL ACV55626.1
Taxonomic identifier479437 [NCBI]
Taxonomic lineageBacteriaActinobacteriaCoriobacteridaeCoriobacterialesCoriobacterineaeCoriobacteriaceaeEggerthella

Protein attributes

Sequence length405 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS By similarity. HAMAP-Rule MF_00021 SAAS SAAS020536

Catalytic activity

L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide. HAMAP-Rule MF_00021 SAAS SAAS020536

[IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP. HAMAP-Rule MF_00021 SAAS SAAS020536

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis. HAMAP-Rule MF_00021 SAAS SAAS020536

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00021 SAAS SAAS020536.

Sequence similarities

Belongs to the ThiI family. HAMAP-Rule MF_00021

Contains 1 THUMP domain. HAMAP-Rule MF_00021

Contains THUMP domain. SAAS SAAS020536

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain66 – 170105THUMP By similarity HAMAP-Rule MF_00021
Nucleotide binding188 – 1892ATP By similarity HAMAP-Rule MF_00021
Nucleotide binding213 – 2142ATP By similarity HAMAP-Rule MF_00021

Sites

Binding site2701ATP By similarity HAMAP-Rule MF_00021
Binding site2921ATP; via amide nitrogen By similarity HAMAP-Rule MF_00021
Binding site3011ATP By similarity HAMAP-Rule MF_00021

Sequences

Sequence LengthMass (Da)Tools
C8WHN5 [UniParc].

Last modified November 3, 2009. Version 1.
Checksum: BAFEA9993BFC7547

FASTA40544,215
        10         20         30         40         50         60 
MTEFQRICLV HYHEIGLKGH NRSTFEMRLL KNLEALLKPF PVVVIHRIAG RLCVFLREGT 

        70         80         90        100        110        120 
DWTTAKEAAD VIGKVPGVAR VSCGFKCERD LDEMTEAALA AMAEAGEFDT FKVAARRNHT 

       130        140        150        160        170        180 
DFATGSMDMN QIIGSALCAA HPEKSVKMKK PDVTVGVEVV QNAAYVYARS LPGVGGLPVG 

       190        200        210        220        230        240 
SSGLVVSLLS SGIDSPVATW KLARRGAVCI GVHFSGRPQT SDASEYLVDD IAQVLERTGC 

       250        260        270        280        290        300 
IARVYAVPFG DYQREIALTV PPELRVIMYR RLMFKVAEEI ARRERAGALV TGESLGQVAS 

       310        320        330        340        350        360 
QTLDNIRCTD AAVDLPVFRP LIGTDKLEII AEAERLGSFE ISSQDAPDCC TLFMPRSPET 

       370        380        390        400 
HAKLPVVLEA EAALPIERWV PEIADAAEVR DYACPAYKPK KKRAS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001726 Genomic DNA. Translation: ACV55626.1.
RefSeqYP_003182015.1. NC_013204.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING479437.Elen_1658.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACV55626; ACV55626; Elen_1658.
GeneID8415957.
KEGGele:Elen_1658.
PATRIC21844015. VBIEggLen106823_1713.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0301.
HOGENOMHOG000227470.
KOK03151.
OrthoDBEOG6TBHGR.

Enzyme and pathway databases

BioCycELEN479437:GHWY-1682-MONOMER.
UniPathwayUPA00060.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00021. ThiI.
InterProIPR014729. Rossmann-like_a/b/a_fold.
IPR020536. ThiI_AANH.
IPR004114. THUMP.
IPR003720. tRNA_STrfase.
[Graphical view]
PfamPF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view]
SMARTSM00981. THUMP. 1 hit.
[Graphical view]
TIGRFAMsTIGR00342. TIGR00342. 1 hit.
PROSITEPS51165. THUMP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC8WHN5_EGGLE
AccessionPrimary (citable) accession number: C8WHN5
Entry history
Integrated into UniProtKB/TrEMBL: November 3, 2009
Last sequence update: November 3, 2009
Last modified: July 9, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)