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C8WHN5

- C8WHN5_EGGLE

UniProt

C8WHN5 - C8WHN5_EGGLE

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Protein
Probable tRNA sulfurtransferase
Gene
thiI, Elen_1658
Organism
Eggerthella lenta (strain ATCC 25559 / DSM 2243 / JCM 9979 / NCTC 11813 / VPI 0255) (Eubacterium lentum)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS By similarity.UniRule annotationSAAS annotations

Catalytic activityi

L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide.UniRule annotationSAAS annotations
[IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotationSAAS annotations

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei270 – 2701ATP By similarityUniRule annotation
Binding sitei292 – 2921ATP; via amide nitrogen By similarityUniRule annotation
Binding sitei301 – 3011ATP By similarityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi188 – 1892ATP By similarityUniRule annotation
Nucleotide bindingi213 – 2142ATP By similarityUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. sulfurtransferase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. tRNA thio-modification Source: UniProtKB-HAMAP
  2. thiamine biosynthetic process Source: UniProtKB-HAMAP
  3. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

TransferaseUniRule annotationSAAS annotations

Keywords - Biological processi

Thiamine biosynthesisUniRule annotationSAAS annotations

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotations, Nucleotide-binding, RNA-binding, tRNA-bindingUniRule annotationSAAS annotations

Enzyme and pathway databases

BioCyciELEN479437:GHWY-1682-MONOMER.
UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA sulfurtransferaseUniRule annotation (EC:2.8.1.4UniRule annotation)
Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferase
Thiamine biosynthesis protein ThiI
tRNA 4-thiouridine synthase
Gene namesi
Name:thiIUniRule annotation
Ordered Locus Names:Elen_1658Imported
OrganismiEggerthella lenta (strain ATCC 25559 / DSM 2243 / JCM 9979 / NCTC 11813 / VPI 0255) (Eubacterium lentum)Imported
Taxonomic identifieri479437 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCoriobacteridaeCoriobacterialesCoriobacterineaeCoriobacteriaceaeEggerthella
ProteomesiUP000001377: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotationSAAS annotations

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotations

Interactioni

Protein-protein interaction databases

STRINGi479437.Elen_1658.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini66 – 170105THUMP By similarityUniRule annotation
Add
BLAST

Sequence similaritiesi

Belongs to the ThiI family.UniRule annotation
Contains 1 THUMP domain.UniRule annotation
Contains THUMP domain.SAAS annotations

Phylogenomic databases

eggNOGiCOG0301.
HOGENOMiHOG000227470.
KOiK03151.
OrthoDBiEOG6TBHGR.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00021. ThiI.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR020536. ThiI_AANH.
IPR004114. THUMP.
IPR003720. tRNA_STrfase.
[Graphical view]
PfamiPF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view]
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00342. TIGR00342. 1 hit.
PROSITEiPS51165. THUMP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C8WHN5-1 [UniParc]FASTAAdd to Basket

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MTEFQRICLV HYHEIGLKGH NRSTFEMRLL KNLEALLKPF PVVVIHRIAG    50
RLCVFLREGT DWTTAKEAAD VIGKVPGVAR VSCGFKCERD LDEMTEAALA 100
AMAEAGEFDT FKVAARRNHT DFATGSMDMN QIIGSALCAA HPEKSVKMKK 150
PDVTVGVEVV QNAAYVYARS LPGVGGLPVG SSGLVVSLLS SGIDSPVATW 200
KLARRGAVCI GVHFSGRPQT SDASEYLVDD IAQVLERTGC IARVYAVPFG 250
DYQREIALTV PPELRVIMYR RLMFKVAEEI ARRERAGALV TGESLGQVAS 300
QTLDNIRCTD AAVDLPVFRP LIGTDKLEII AEAERLGSFE ISSQDAPDCC 350
TLFMPRSPET HAKLPVVLEA EAALPIERWV PEIADAAEVR DYACPAYKPK 400
KKRAS 405
Length:405
Mass (Da):44,215
Last modified:November 3, 2009 - v1
Checksum:iBAFEA9993BFC7547
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001726 Genomic DNA. Translation: ACV55626.1.
RefSeqiYP_003182015.1. NC_013204.1.

Genome annotation databases

EnsemblBacteriaiACV55626; ACV55626; Elen_1658.
GeneIDi8415957.
KEGGiele:Elen_1658.
PATRICi21844015. VBIEggLen106823_1713.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001726 Genomic DNA. Translation: ACV55626.1 .
RefSeqi YP_003182015.1. NC_013204.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 479437.Elen_1658.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACV55626 ; ACV55626 ; Elen_1658 .
GeneIDi 8415957.
KEGGi ele:Elen_1658.
PATRICi 21844015. VBIEggLen106823_1713.

Phylogenomic databases

eggNOGi COG0301.
HOGENOMi HOG000227470.
KOi K03151.
OrthoDBi EOG6TBHGR.

Enzyme and pathway databases

UniPathwayi UPA00060 .
BioCyci ELEN479437:GHWY-1682-MONOMER.

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
HAMAPi MF_00021. ThiI.
InterProi IPR014729. Rossmann-like_a/b/a_fold.
IPR020536. ThiI_AANH.
IPR004114. THUMP.
IPR003720. tRNA_STrfase.
[Graphical view ]
Pfami PF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view ]
SMARTi SM00981. THUMP. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00342. TIGR00342. 1 hit.
PROSITEi PS51165. THUMP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25559 / DSM 2243 / JCM 9979 / NCTC 11813 / VPI 0255.

Entry informationi

Entry nameiC8WHN5_EGGLE
AccessioniPrimary (citable) accession number: C8WHN5
Entry historyi
Integrated into UniProtKB/TrEMBL: November 3, 2009
Last sequence update: November 3, 2009
Last modified: July 9, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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