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C8WHN5

- C8WHN5_EGGLE

UniProt

C8WHN5 - C8WHN5_EGGLE

Protein

Probable tRNA sulfurtransferase

Gene

thiI

Organism
Eggerthella lenta (strain ATCC 25559 / DSM 2243 / JCM 9979 / NCTC 11813 / VPI 0255) (Eubacterium lentum)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 37 (01 Oct 2014)
      Sequence version 1 (03 Nov 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.UniRule annotationSAAS annotation

    Catalytic activityi

    L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide.UniRule annotationSAAS annotation
    [IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotationSAAS annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei270 – 2701ATPUniRule annotation
    Binding sitei292 – 2921ATP; via amide nitrogenUniRule annotation
    Binding sitei301 – 3011ATPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi188 – 1892ATPUniRule annotation
    Nucleotide bindingi213 – 2142ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. sulfurtransferase activity Source: UniProtKB-HAMAP
    3. tRNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. thiamine biosynthetic process Source: UniProtKB-HAMAP
    2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
    3. tRNA thio-modification Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    TransferaseUniRule annotationSAAS annotation

    Keywords - Biological processi

    Thiamine biosynthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding, RNA-binding, tRNA-bindingUniRule annotationSAAS annotation

    Enzyme and pathway databases

    BioCyciELEN479437:GHWY-1682-MONOMER.
    UniPathwayiUPA00060.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable tRNA sulfurtransferaseUniRule annotation (EC:2.8.1.4UniRule annotation)
    Alternative name(s):
    Sulfur carrier protein ThiS sulfurtransferaseUniRule annotation
    Thiamine biosynthesis protein ThiIUniRule annotation
    tRNA 4-thiouridine synthaseUniRule annotation
    Gene namesi
    Name:thiIUniRule annotation
    Ordered Locus Names:Elen_1658Imported
    OrganismiEggerthella lenta (strain ATCC 25559 / DSM 2243 / JCM 9979 / NCTC 11813 / VPI 0255) (Eubacterium lentum)Imported
    Taxonomic identifieri479437 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaCoriobacteridaeCoriobacterialesCoriobacterineaeCoriobacteriaceaeEggerthella
    ProteomesiUP000001377: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    Interactioni

    Protein-protein interaction databases

    STRINGi479437.Elen_1658.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini66 – 170105THUMPUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ThiI family.UniRule annotation
    Contains 1 THUMP domain.UniRule annotation
    Contains THUMP domain.SAAS annotation

    Phylogenomic databases

    eggNOGiCOG0301.
    HOGENOMiHOG000227470.
    KOiK03151.
    OrthoDBiEOG6TBHGR.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    HAMAPiMF_00021. ThiI.
    InterProiIPR014729. Rossmann-like_a/b/a_fold.
    IPR020536. ThiI_AANH.
    IPR004114. THUMP.
    IPR003720. tRNA_STrfase.
    [Graphical view]
    PfamiPF02568. ThiI. 1 hit.
    PF02926. THUMP. 1 hit.
    [Graphical view]
    SMARTiSM00981. THUMP. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00342. TIGR00342. 1 hit.
    PROSITEiPS51165. THUMP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C8WHN5-1 [UniParc]FASTAAdd to Basket

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    MTEFQRICLV HYHEIGLKGH NRSTFEMRLL KNLEALLKPF PVVVIHRIAG    50
    RLCVFLREGT DWTTAKEAAD VIGKVPGVAR VSCGFKCERD LDEMTEAALA 100
    AMAEAGEFDT FKVAARRNHT DFATGSMDMN QIIGSALCAA HPEKSVKMKK 150
    PDVTVGVEVV QNAAYVYARS LPGVGGLPVG SSGLVVSLLS SGIDSPVATW 200
    KLARRGAVCI GVHFSGRPQT SDASEYLVDD IAQVLERTGC IARVYAVPFG 250
    DYQREIALTV PPELRVIMYR RLMFKVAEEI ARRERAGALV TGESLGQVAS 300
    QTLDNIRCTD AAVDLPVFRP LIGTDKLEII AEAERLGSFE ISSQDAPDCC 350
    TLFMPRSPET HAKLPVVLEA EAALPIERWV PEIADAAEVR DYACPAYKPK 400
    KKRAS 405
    Length:405
    Mass (Da):44,215
    Last modified:November 3, 2009 - v1
    Checksum:iBAFEA9993BFC7547
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001726 Genomic DNA. Translation: ACV55626.1.
    RefSeqiYP_003182015.1. NC_013204.1.

    Genome annotation databases

    EnsemblBacteriaiACV55626; ACV55626; Elen_1658.
    GeneIDi8415957.
    KEGGiele:Elen_1658.
    PATRICi21844015. VBIEggLen106823_1713.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001726 Genomic DNA. Translation: ACV55626.1 .
    RefSeqi YP_003182015.1. NC_013204.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 479437.Elen_1658.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACV55626 ; ACV55626 ; Elen_1658 .
    GeneIDi 8415957.
    KEGGi ele:Elen_1658.
    PATRICi 21844015. VBIEggLen106823_1713.

    Phylogenomic databases

    eggNOGi COG0301.
    HOGENOMi HOG000227470.
    KOi K03151.
    OrthoDBi EOG6TBHGR.

    Enzyme and pathway databases

    UniPathwayi UPA00060 .
    BioCyci ELEN479437:GHWY-1682-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    HAMAPi MF_00021. ThiI.
    InterProi IPR014729. Rossmann-like_a/b/a_fold.
    IPR020536. ThiI_AANH.
    IPR004114. THUMP.
    IPR003720. tRNA_STrfase.
    [Graphical view ]
    Pfami PF02568. ThiI. 1 hit.
    PF02926. THUMP. 1 hit.
    [Graphical view ]
    SMARTi SM00981. THUMP. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00342. TIGR00342. 1 hit.
    PROSITEi PS51165. THUMP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 25559 / DSM 2243 / JCM 9979 / NCTC 11813 / VPI 0255Imported.

    Entry informationi

    Entry nameiC8WHN5_EGGLE
    AccessioniPrimary (citable) accession number: C8WHN5
    Entry historyi
    Integrated into UniProtKB/TrEMBL: November 3, 2009
    Last sequence update: November 3, 2009
    Last modified: October 1, 2014
    This is version 37 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3