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C8WHN5

- C8WHN5_EGGLE

UniProt

C8WHN5 - C8WHN5_EGGLE

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Protein

Probable tRNA sulfurtransferase

Gene

thiI

Organism
Eggerthella lenta (strain ATCC 25559 / DSM 2243 / JCM 9979 / NCTC 11813 / VPI 0255) (Eubacterium lentum)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.UniRule annotationSAAS annotation

Catalytic activityi

L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide.UniRule annotationSAAS annotation
[IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotationSAAS annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei270 – 2701ATPUniRule annotation
Binding sitei292 – 2921ATP; via amide nitrogenUniRule annotation
Binding sitei301 – 3011ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi188 – 1892ATPUniRule annotation
Nucleotide bindingi213 – 2142ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. sulfurtransferase activity Source: UniProtKB-HAMAP
  3. tRNA adenylyltransferase activity Source: InterPro
  4. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. thiamine biosynthetic process Source: UniProtKB-HAMAP
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
  3. tRNA thio-modification Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

TransferaseUniRule annotationSAAS annotation

Keywords - Biological processi

Thiamine biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding, RNA-binding, tRNA-bindingUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciELEN479437:GHWY-1682-MONOMER.
UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA sulfurtransferaseUniRule annotation (EC:2.8.1.4UniRule annotation)
Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferaseUniRule annotation
Thiamine biosynthesis protein ThiIUniRule annotation
tRNA 4-thiouridine synthaseUniRule annotation
Gene namesi
Name:thiIUniRule annotation
Ordered Locus Names:Elen_1658Imported
OrganismiEggerthella lenta (strain ATCC 25559 / DSM 2243 / JCM 9979 / NCTC 11813 / VPI 0255) (Eubacterium lentum)Imported
Taxonomic identifieri479437 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCoriobacteridaeCoriobacterialesCoriobacterineaeCoriobacteriaceaeEggerthella
ProteomesiUP000001377: Chromosome

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Protein-protein interaction databases

STRINGi479437.Elen_1658.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini66 – 170105THUMPUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ThiI family.UniRule annotation
Contains 1 THUMP domain.UniRule annotation
Contains THUMP domain.SAAS annotation

Phylogenomic databases

eggNOGiCOG0301.
HOGENOMiHOG000227470.
KOiK03151.
OrthoDBiEOG6TBHGR.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00021. ThiI.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR020536. ThiI_AANH.
IPR004114. THUMP.
IPR003720. tRNA_STrfase.
[Graphical view]
PfamiPF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view]
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00342. TIGR00342. 1 hit.
PROSITEiPS51165. THUMP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C8WHN5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTEFQRICLV HYHEIGLKGH NRSTFEMRLL KNLEALLKPF PVVVIHRIAG
60 70 80 90 100
RLCVFLREGT DWTTAKEAAD VIGKVPGVAR VSCGFKCERD LDEMTEAALA
110 120 130 140 150
AMAEAGEFDT FKVAARRNHT DFATGSMDMN QIIGSALCAA HPEKSVKMKK
160 170 180 190 200
PDVTVGVEVV QNAAYVYARS LPGVGGLPVG SSGLVVSLLS SGIDSPVATW
210 220 230 240 250
KLARRGAVCI GVHFSGRPQT SDASEYLVDD IAQVLERTGC IARVYAVPFG
260 270 280 290 300
DYQREIALTV PPELRVIMYR RLMFKVAEEI ARRERAGALV TGESLGQVAS
310 320 330 340 350
QTLDNIRCTD AAVDLPVFRP LIGTDKLEII AEAERLGSFE ISSQDAPDCC
360 370 380 390 400
TLFMPRSPET HAKLPVVLEA EAALPIERWV PEIADAAEVR DYACPAYKPK

KKRAS
Length:405
Mass (Da):44,215
Last modified:November 3, 2009 - v1
Checksum:iBAFEA9993BFC7547
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001726 Genomic DNA. Translation: ACV55626.1.
RefSeqiYP_003182015.1. NC_013204.1.

Genome annotation databases

EnsemblBacteriaiACV55626; ACV55626; Elen_1658.
GeneIDi8415957.
KEGGiele:Elen_1658.
PATRICi21844015. VBIEggLen106823_1713.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001726 Genomic DNA. Translation: ACV55626.1 .
RefSeqi YP_003182015.1. NC_013204.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 479437.Elen_1658.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACV55626 ; ACV55626 ; Elen_1658 .
GeneIDi 8415957.
KEGGi ele:Elen_1658.
PATRICi 21844015. VBIEggLen106823_1713.

Phylogenomic databases

eggNOGi COG0301.
HOGENOMi HOG000227470.
KOi K03151.
OrthoDBi EOG6TBHGR.

Enzyme and pathway databases

UniPathwayi UPA00060 .
BioCyci ELEN479437:GHWY-1682-MONOMER.

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
HAMAPi MF_00021. ThiI.
InterProi IPR014729. Rossmann-like_a/b/a_fold.
IPR020536. ThiI_AANH.
IPR004114. THUMP.
IPR003720. tRNA_STrfase.
[Graphical view ]
Pfami PF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view ]
SMARTi SM00981. THUMP. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00342. TIGR00342. 1 hit.
PROSITEi PS51165. THUMP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25559 / DSM 2243 / JCM 9979 / NCTC 11813 / VPI 0255Imported.

Entry informationi

Entry nameiC8WHN5_EGGLE
AccessioniPrimary (citable) accession number: C8WHN5
Entry historyi
Integrated into UniProtKB/TrEMBL: November 3, 2009
Last sequence update: November 3, 2009
Last modified: October 29, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3