C8W659 (C8W659_DESAS) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Acetolactate synthase RuleBase RU003591
EC=2.2.1.6 RuleBase RU003591

Gene names

Ordered Locus Names:

Dtox_0594

Organism

Desulfotomaculum acetoxidans (strain ATCC 49208 / DSM 771 / VKM B-1644) [Complete proteome] [HAMAP] EMBL ACV61514.1

Taxonomic identifier

485916 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Peptococcaceae › Desulfotomaculum › Desulfotomaculum acetoxidans

Protein attributes

Sequence length	557 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2 pyruvate = 2-acetolactate + CO2. RuleBase RU003591
Cofactor	Binds 1 magnesium ion per subunit By similarity. RuleBase RU003591 Binds 1 thiamine pyrophosphate per subunit By similarity. RuleBase RU003591
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4. RuleBase RU003591 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4. RuleBase RU003591
Sequence similarities	Belongs to the TPP enzyme family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis RuleBase RU003591
Ligand	Magnesium RuleBase RU003591 Metal-binding RuleBase RU003591 Thiamine pyrophosphate RuleBase RU003591
Molecular function	Transferase RuleBase RU003591
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	isoleucine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway valine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	acetolactate synthase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: InterPro thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	C8W659 [UniParc]. Last modified November 3, 2009. Version 1. Checksum: 32B28592A7F908D7	FASTA	557	60,345
	10 20 30 40 50 60 MGITVAEALV LCLERENVEV IFGYPGGAIL PVYDALYKSN KIKHVLVRHE QGAAHAADGY 70 80 90 100 110 120 ARATGKVGVC MATSGPGATN LVTGIANAYM DSVPIVAFTG QVPTGMVGTD AFQEVDITGI 130 140 150 160 170 180 TMPVVKHNYF VKDPKQVPKI VREAFYIART GRPGPVLIDL PKDVTSAIID YEEVEEINLR 190 200 210 220 230 240 GYKPNYRADD EQVEKAAEMI KKSQCPVIYA GGGVVSAGAH DELKVLAETI GAPVTHTLLG 250 260 270 280 290 300 LGGFPGDHPL FAGMLGLHGA MYANKIVTEC DLLITLGARF DDRVTGKIAG FAPDASVIHV 310 320 330 340 350 360 DIDPAEIGKN VRVHLPVAGD VKTVIQQIIP LLEQKNNRQW LERFAELKKQ YALRYEPAAG 370 380 390 400 410 420 EGLKPQQVIE KLNEVAQGNA IICTDVGQHQ MWAAHYYRHI QPRSFISSGG LGTMGYGYPA 430 440 450 460 470 480 AIGAEFGCPG KQVILISGDG SFQMNLQEMA TVMEQGLSVK VIVLNNGRLG MVRQLQEFYC 490 500 510 520 530 540 DKRYMAVDFS YHPDFAGIAR VYGMKGFDIK TSEELNAILP EVLSSQGPAI INCFVQEDEN 550 VYPMVMAGKC IDEAIEG

References

[1]

"Complete genome sequence of Desulfotomaculum acetoxidans type strain (5575)." Spring S., Lapidus A., Schroder M., Gleim D., Sims D., Meincke L., Glavina Del Rio T., Tice H., Copeland A., Cheng J.F., Lucas S., Chen F., Nolan M., Bruce D., Goodwin L., Pitluck S., Ivanova N., Mavromatis K. , Mikhailova N., Pati A., Chen A., Palaniappan K., Land M., Hauser L., Chang Y.J., Jeffries C.D., Chain P., Saunders E., Brettin T., Detter J.C., Goker M., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P., Kyrpides N.C., Klenk H.P., Han C. Stand. Genomic Sci. 1:242-253(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49208 / DSM 771 / VKM B-1644.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001720 Genomic DNA. Translation: ACV61514.1.
RefSeq	YP_003190137.1. NC_013216.1.
3D structure databases
ProteinModelPortal	C8W659.
ModBase	Search...
Protein-protein interaction databases
STRING	485916.Dtox_0594.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACV61514; ACV61514; Dtox_0594.
GeneID	8427529.
KEGG	dae:Dtox_0594.
PATRIC	21717891. VBIDesAce42372_0601.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0028.
HOGENOM	HOG000258448.
KO	K01652.
OMA	CLERENV.
ProtClustDB	CLSK841846.
Enzyme and pathway databases
BioCyc	DACE485916:GHUF-610-MONOMER.
UniPathway	UPA00047; UER00055. UPA00049; UER00059.
Family and domain databases
InterPro	IPR012846. Acetolactate_synth_lsu. IPR012000. Thiamin_PyroP_enz_cen_dom. IPR012001. Thiamin_PyroP_enz_TPP-bd_dom. IPR000399. TPP-bd_CS. IPR011766. TPP_enzyme-bd_C. [Graphical view]
PANTHER	PTHR18968:SF13. PTHR18968:SF13. 1 hit.
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF00205. TPP_enzyme_M. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00118. acolac_lg. 1 hit.
PROSITE	PS00187. TPP_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C8W659_DESAS

Accession

Primary (citable) accession number: C8W659

Entry history

Integrated into UniProtKB/TrEMBL:	November 3, 2009
Last sequence update:	November 3, 2009
Last modified:	May 1, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)