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Protein

Glutamate 5-kinase

Gene

proB

Organism
Desulfotomaculum acetoxidans (strain ATCC 49208 / DSM 771 / VKM B-1644)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotationSAAS annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotationSAAS annotation

Pathway:iL-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotationSAAS annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei15 – 151ATPUniRule annotation
Binding sitei55 – 551SubstrateUniRule annotation
Binding sitei142 – 1421SubstrateUniRule annotation
Binding sitei154 – 1541Substrate; via amide nitrogenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi174 – 1752ATPUniRule annotation
Nucleotide bindingi216 – 2227ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationSAAS annotation, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciDACE485916:GHUF-3386-MONOMER.
UniPathwayiUPA00098; UER00359.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotationSAAS annotation (EC:2.7.2.11UniRule annotationSAAS annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:Dtox_3311Imported
OrganismiDesulfotomaculum acetoxidans (strain ATCC 49208 / DSM 771 / VKM B-1644)Imported
Taxonomic identifieri485916 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptococcaceaeDesulfotomaculum
ProteomesiUP000002217 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Protein-protein interaction databases

STRINGi485916.Dtox_3311.

Structurei

3D structure databases

ProteinModelPortaliC8W5P2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini280 – 35879PUAUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation
Contains 1 PUA domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0263.
HOGENOMiHOG000246369.
KOiK00931.
OMAiREPRIAN.
OrthoDBiEOG6PGK7G.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C8W5P2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNRDFSRVK RLVVKVGSSS LAHATGKLNL YQFEQLAVQL ADLHNQGKEI
60 70 80 90 100
LLVTSGAVGA GLGKLGLRRR PKTMPEKQAA AAVGQGVLMQ MYEKFFAEYG
110 120 130 140 150
VTVGQVLLSR EDFSDRRRFL NARNTMYALL GMGVIPIINE NDTVSIEEIR
160 170 180 190 200
LGDNDTLSAL VTGLVDAELL VLLSDIDGVY TADPRKDPAA VIISEIDEIN
210 220 230 240 250
EQVESLAGGA GSGLGTGGMT TKLQAAKIAM RSGAAMVIAR AAEKNIIRRV
260 270 280 290 300
VTGEPLGTVF CPGNKLDNKK HWIMYSSAVQ GKISIDEGAS RALVKNGKSL
310 320 330 340 350
LPSGVVKVEG VFEMGNTVCI SGPDGRQIAR GITNYAAEEL KKIMGKKTSE
360 370
IAVVLGSKDY DEVIHRNNLV LDC
Length:373
Mass (Da):40,031
Last modified:November 3, 2009 - v1
Checksum:i1C5C85B12B66C744
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001720 Genomic DNA. Translation: ACV64042.1.

Genome annotation databases

EnsemblBacteriaiACV64042; ACV64042; Dtox_3311.
KEGGidae:Dtox_3311.
PATRICi21723531. VBIDesAce42372_3366.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001720 Genomic DNA. Translation: ACV64042.1.

3D structure databases

ProteinModelPortaliC8W5P2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi485916.Dtox_3311.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACV64042; ACV64042; Dtox_3311.
KEGGidae:Dtox_3311.
PATRICi21723531. VBIDesAce42372_3366.

Phylogenomic databases

eggNOGiCOG0263.
HOGENOMiHOG000246369.
KOiK00931.
OMAiREPRIAN.
OrthoDBiEOG6PGK7G.

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.
BioCyciDACE485916:GHUF-3386-MONOMER.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49208 / DSM 771 / VKM B-1644Imported.

Entry informationi

Entry nameiC8W5P2_DESAS
AccessioniPrimary (citable) accession number: C8W5P2
Entry historyi
Integrated into UniProtKB/TrEMBL: November 3, 2009
Last sequence update: November 3, 2009
Last modified: July 22, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.