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Protein

S-methyl-5'-thioadenosine phosphorylase

Gene

AN10230

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. S-methyl-5'-thioadenosine phosphorylase (AN10230)
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route), the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei45PhosphateUniRule annotation1
Sitei208Important for substrate specificityUniRule annotation1
Binding sitei226Substrate; via amide nitrogenUniRule annotation1
Binding sitei227PhosphateUniRule annotation1
Sitei263Important for substrate specificityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPurine salvage

Enzyme and pathway databases

UniPathwayiUPA00904; UER00873

Names & Taxonomyi

Protein namesi
Recommended name:
S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)
Alternative name(s):
5'-methylthioadenosine phosphorylaseUniRule annotation
Short name:
MTA phosphorylaseUniRule annotation
Short name:
MTAPUniRule annotation
Short name:
MTAPaseUniRule annotation
Gene namesi
ORF Names:AN10230
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000000560 Componenti: Chromosome VII
  • UP000005890 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004151281 – 355S-methyl-5'-thioadenosine phosphorylaseAdd BLAST355

Proteomic databases

PRIDEiC8VP37

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi162425.CADANIAP00008381

Structurei

3D structure databases

ProteinModelPortaliC8VP37
SMRiC8VP37
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni91 – 92Phosphate bindingUniRule annotation2
Regioni124 – 125Phosphate bindingUniRule annotation2
Regioni250 – 252Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000228986
InParanoidiC8VP37
KOiK00033
OMAiNLYRSWG
OrthoDBiEOG092C46SL

Family and domain databases

HAMAPiMF_01963 MTAP, 1 hit
InterProiView protein in InterPro
IPR010044 MTAP
IPR000845 Nucleoside_phosphorylase_d
IPR035994 Nucleoside_phosphorylase_sf
IPR018099 Purine_phosphorylase-2_CS
PANTHERiPTHR42679 PTHR42679, 1 hit
PfamiView protein in Pfam
PF01048 PNP_UDP_1, 1 hit
SUPFAMiSSF53167 SSF53167, 1 hit
TIGRFAMsiTIGR01694 MTAP, 1 hit
PROSITEiView protein in PROSITE
PS01240 PNP_MTAP_2, 1 hit

Sequencei

Sequence statusi: Complete.

C8VP37-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLRSVSSIT KPFRRIESPK QLARRMAVLP DTYADPVRIA VIGGTGLREL
60 70 80 90 100
PGFTQVASLN IQTPWGTPSS PITILHHTHK DKTVAVAFLS RHGLHHQIAP
110 120 130 140 150
HEVPARANIA ALRSIGVRTI IAFSAVGSLQ EEIKPRDFVV PDQVIDRTKG
160 170 180 190 200
IRPFTFFEGG VVGHVPFGDP FDESVAKIVR ACGHSLEGEG VKLHDRGTLI
210 220 230 240 250
CMEGPQFSTR AESKLYRSWG GSVINMSCLP EAKLAREAEI AYQMICMSTD
260 270 280 290 300
YDCWHESTED VTVEMVMGNM KANAVNAKHF VTAVLDELAD DRNAELVQAK
310 320 330 340 350
QYAGSVKFGL STAQANWSPE ARERMNWLFP GYFEIDLYIR YLLLIQRDID

LTSWN
Length:355
Mass (Da):39,550
Last modified:November 3, 2009 - v1
Checksum:i6B969656E0F2F572
GO

Sequence cautioni

The sequence EAA64021 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene AN1735 has been split into 2 genes: AN10230 and AN10233.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACD01000027 Genomic DNA Translation: EAA64021.1 Sequence problems.
BN001307 Genomic DNA Translation: CBF85464.1
RefSeqiXP_659339.1, XM_654247.1

Genome annotation databases

EnsemblFungiiCADANIAT00008381; CADANIAP00008381; CADANIAG00008381
EAA64021; EAA64021; AN1735.2
GeneIDi2875718
KEGGiani:AN1735.2

Similar proteinsi

Entry informationi

Entry nameiMTAP_EMENI
AccessioniPrimary (citable) accession number: C8VP37
Secondary accession number(s): Q5BCJ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: November 3, 2009
Last modified: May 23, 2018
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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