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Protein

Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutB

Gene

rutB

Organism
Escherichia coli O111:H- (strain 11128 / EHEC)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby releasing one of the pyrimidine rings nitrogen atoms as ammonia and one of its carbons as CO2.UniRule annotation

Catalytic activityi

(Z)-3-ureidoacrylate peracid + H2O = (Z)-3-peroxyaminoacrylate + CO2 + NH3.UniRule annotation
(Z)-3-ureidoacrylate + H2O = (Z)-3-aminoacrylate + CO2 + NH3.UniRule annotation
(Z)-2-methyl-ureidoacrylate peracid + H2O = (Z)-2-methyl-peroxyaminoacrylate + CO2 + NH3.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei166NucleophileUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutBUniRule annotation (EC:3.5.1.110UniRule annotation)
Alternative name(s):
Ureidoacrylate amidohydrolaseUniRule annotation
Gene namesi
Name:rutBUniRule annotation
Ordered Locus Names:ECO111_1200
OrganismiEscherichia coli O111:H- (strain 11128 / EHEC)
Taxonomic identifieri585396 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000001614 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004026681 – 230Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutBAdd BLAST230

Expressioni

Inductioni

Up-regulated by the nitrogen regulatory protein C (NtrC also called GlnG) and repressed by RutR.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliC8UMM7.
SMRiC8UMM7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the isochorismatase family. RutB subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000078669.
KOiK09020.
OMAiWHKSNAL.

Family and domain databases

HAMAPiMF_00830. RutB. 1 hit.
InterProiView protein in InterPro
IPR019916. IsoChorase_RutB.
IPR000868. Isochorismatase-like.
PfamiView protein in Pfam
PF00857. Isochorismatase. 1 hit.
SUPFAMiSSF52499. SSF52499. 1 hit.
TIGRFAMsiTIGR03614. RutB. 1 hit.

Sequencei

Sequence statusi: Complete.

C8UMM7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTLTARPEA ITFDPQQSAL IVVDMQNAYA TPGGYLDLAG FDVSTTRPVI
60 70 80 90 100
ANIQTAVTAA RAAGMLIIWF QNGWDEQYVE AGGPGSPNFH KSNALKTMRK
110 120 130 140 150
QPQLQGKLLA KGSWDYQLVD ELVPQPGDIV LPKPRYSGFF NTPLDSILRS
160 170 180 190 200
RGIRHLVFTG IATNVCVEST LRDGFFLEYF GVVLEDATHQ AGPEFAQKAA
210 220 230
LFNIETFFGW VSDVETFCDA LSPTSFARIA
Length:230
Mass (Da):25,229
Last modified:November 3, 2009 - v1
Checksum:i6E2647C2DB88E4A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP010960 Genomic DNA. Translation: BAI35147.1.
RefSeqiWP_001307708.1. NC_013364.1.

Genome annotation databases

EnsemblBacteriaiBAI35147; BAI35147; ECO111_1200.
KEGGieoi:ECO111_1200.

Similar proteinsi

Entry informationi

Entry nameiRUTB_ECO1A
AccessioniPrimary (citable) accession number: C8UMM7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: November 3, 2009
Last modified: August 30, 2017
This is version 46 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families