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C8U8V8

- C8U8V8_ECO10

UniProt

C8U8V8 - C8U8V8_ECO10

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Protein

3-phenylpropionate/cinnamic acid dioxygenase ferredoxin--NAD(+) reductase component

Gene

hcaD

Organism
Escherichia coli O103:H2 (strain 12009 / EHEC)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Part of the multicomponent 3-phenylpropionate dioxygenase, that converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively.UniRule annotation

Catalytic activityi

Reduced ferredoxin + NAD+ = oxidized ferredoxin + NADH.UniRule annotation

Cofactori

FAD.UniRule annotation

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi5 – 3632FADUniRule annotationAdd
BLAST
Nucleotide bindingi146 – 17429NADUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. 3-phenylpropionate dioxygenase activity Source: UniProtKB-HAMAP
  2. ferredoxin-NAD+ reductase activity Source: UniProtKB-EC
  3. flavin adenine dinucleotide binding Source: InterPro

GO - Biological processi

  1. 3-phenylpropionate catabolic process Source: UniProtKB-UniPathway
  2. cell redox homeostasis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

DioxygenaseImported, Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolismUniRule annotation

Keywords - Ligandi

FADUniRule annotation, Flavoprotein, NADUniRule annotation

Enzyme and pathway databases

BioCyciECOL585395:GJA9-3161-MONOMER.
UniPathwayiUPA00714.

Names & Taxonomyi

Protein namesi
Recommended name:
3-phenylpropionate/cinnamic acid dioxygenase ferredoxin--NAD(+) reductase componentUniRule annotation (EC:1.18.1.3UniRule annotation)
Alternative name(s):
Digoxigenin system ferredoxin--NAD(+) reductase componentUniRule annotation
Gene namesi
Name:hcaDUniRule annotationImported
Ordered Locus Names:ECO103_3062Imported
OrganismiEscherichia coli O103:H2 (strain 12009 / EHEC)Imported
Taxonomic identifieri585395 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000959: Chromosome

Interactioni

Subunit structurei

This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (HcaE and HcaF), a ferredoxin (HcaC) and a ferredoxin reductase (HcaD).UniRule annotation

Protein-protein interaction databases

STRINGi585395.ECO103_3062.

Structurei

3D structure databases

ProteinModelPortaliC8U8V8.
SMRiC8U8V8. Positions 4-400.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the bacterial ring-hydroxylating dioxygenase ferredoxin reductase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0446.
HOGENOMiHOG000276711.
KOiK00529.
OMAiRLPPPWF.
OrthoDBiEOG6T4RXM.

Family and domain databases

Gene3Di3.30.390.30. 1 hit.
HAMAPiMF_01651. HcaD.
InterProiIPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR023744. HcaD.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
IPR028202. Reductase_C.
[Graphical view]
PfamiPF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF14759. Reductase_C. 1 hit.
[Graphical view]
PRINTSiPR00368. FADPNR.
SUPFAMiSSF55424. SSF55424. 1 hit.

Sequencei

Sequence statusi: Complete.

C8U8V8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKEKTIIIVG GGQAAAMAAA SLRQQGFTGE LHLFSDERHL PYERPPLSKS
60 70 80 90 100
MLLEDSPQLQ QVLPANWWQE NNVHLHSGVT IKTLGRDTRE LVLTNGESWH
110 120 130 140 150
WDQLFIATGA AARPLPLLDA LGERCFTLRH AGDAARLREV LQPERSVVIV
160 170 180 190 200
GAGTIGLELA ASATQRRCKV TVIELAATVM GRNAPPPVQR YLLQRHQQAG
210 220 230 240 250
VRILLNNAIE HVVDGEKVEL TLQSGETLQA DVVIYGIGIS ANEQLAREAN
260 270 280 290 300
LDTANGIVID EACRTCDPAI FAGGDVAITR LDNGALHRCE SWENANNQAQ
310 320 330 340 350
IAAAAMLGLP LPLLPPPWFW SDQYSDNLQF IGDMRGDDWL CRGNPETQKA
360 370 380 390 400
IWFNLQNGVL IGAVTLNQGR EIRPIRKWIQ SGKTFDAKLL IDENIALKSL
Length:400
Mass (Da):43,964
Last modified:November 3, 2009 - v1
Checksum:iF5A1A7CF4F1DF2A6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP010958 Genomic DNA. Translation: BAI31817.1.
RefSeqiYP_003222951.1. NC_013353.1.

Genome annotation databases

EnsemblBacteriaiBAI31817; BAI31817; ECO103_3062.
GeneIDi8475575.
KEGGieoh:ECO103_3062.
PATRICi32098074. VBIEscCol146794_3208.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP010958 Genomic DNA. Translation: BAI31817.1 .
RefSeqi YP_003222951.1. NC_013353.1.

3D structure databases

ProteinModelPortali C8U8V8.
SMRi C8U8V8. Positions 4-400.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 585395.ECO103_3062.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAI31817 ; BAI31817 ; ECO103_3062 .
GeneIDi 8475575.
KEGGi eoh:ECO103_3062.
PATRICi 32098074. VBIEscCol146794_3208.

Phylogenomic databases

eggNOGi COG0446.
HOGENOMi HOG000276711.
KOi K00529.
OMAi RLPPPWF.
OrthoDBi EOG6T4RXM.

Enzyme and pathway databases

UniPathwayi UPA00714 .
BioCyci ECOL585395:GJA9-3161-MONOMER.

Family and domain databases

Gene3Di 3.30.390.30. 1 hit.
HAMAPi MF_01651. HcaD.
InterProi IPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR023744. HcaD.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
IPR028202. Reductase_C.
[Graphical view ]
Pfami PF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF14759. Reductase_C. 1 hit.
[Graphical view ]
PRINTSi PR00368. FADPNR.
SUPFAMi SSF55424. SSF55424. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Comparative genomics reveal the mechanism of the parallel evolution of O157 and non-O157 enterohemorrhagic Escherichia coli."
    Ogura Y., Ooka T., Iguchi A., Toh H., Asadulghani M., Oshima K., Kodama T., Abe H., Nakayama K., Kurokawa K., Tobe T., Hattori M., Hayashi T.
    Proc. Natl. Acad. Sci. U.S.A. 106:17939-17944(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 12009 / EHECImported.

Entry informationi

Entry nameiC8U8V8_ECO10
AccessioniPrimary (citable) accession number: C8U8V8
Entry historyi
Integrated into UniProtKB/TrEMBL: November 3, 2009
Last sequence update: November 3, 2009
Last modified: October 1, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3