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Protein

Alanine racemase

Gene

dadX

Organism
Escherichia coli O26:H11 (strain 11368 / EHEC)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids.UniRule annotation

Catalytic activityi

L-alanine = D-alanine.UniRule annotationSAAS annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotationSAAS annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei35 – 351Proton acceptor; specific for D-alanineUniRule annotation
Binding sitei130 – 1301SubstrateUniRule annotation
Active sitei253 – 2531Proton acceptor; specific for L-alanineUniRule annotation
Binding sitei301 – 3011Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

  1. alanine racemase activity Source: UniProtKB-HAMAP
  2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. D-alanine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

IsomeraseUniRule annotationSAAS annotation

Keywords - Ligandi

Pyridoxal phosphateUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciECOL573235:GCY7-1750-MONOMER.
UniPathwayiUPA00042; UER00497.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine racemaseUniRule annotationSAAS annotation (EC:5.1.1.1UniRule annotationSAAS annotation)
Gene namesi
Name:dadXImported
Ordered Locus Names:ECO26_1703Imported
OrganismiEscherichia coli O26:H11 (strain 11368 / EHEC)Imported
Taxonomic identifieri573235 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000001617: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei35 – 351N6-(pyridoxal phosphate)lysineUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi573235.ECO26_1703.

Structurei

3D structure databases

ProteinModelPortaliC8TQK2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alanine racemase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0787.
HOGENOMiHOG000031446.
KOiK01775.
OrthoDBiEOG6PP9NJ.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_01201. Ala_racemase.
InterProiIPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR020622. Ala_racemase_pyridoxalP-BS.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]
PRINTSiPR00992. ALARACEMASE.
SMARTiSM01005. Ala_racemase_C. 1 hit.
[Graphical view]
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR00492. alr. 1 hit.
PROSITEiPS00395. ALANINE_RACEMASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C8TQK2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRPIQASLD LQALKQNLSI VRQAAPHARV WSVVKANAYG HGIERIWSAL
60 70 80 90 100
GATDGFALLN LEEAITLRER GWKGPILMLE GFFHAQDLEI YDQHRLTTCV
110 120 130 140 150
HSNWQLKALQ NARLKAPLDI YLKVNSGMNR LGFQPDRVLT VWQQLRAMAN
160 170 180 190 200
VGEMTLMSHF AEAEHPDGIS SAMARIEQAA EGLECRRSLS NSAATLWHPE
210 220 230 240 250
AHFDWVRPGI ILYGASPSGQ WRDIANTGLR PVMTLSSEII GVQTLKAGER
260 270 280 290 300
VGYGGRYTAR DEQRIGIVAA GYADGYPRHA PTGTPVLVDG VRTMTVGTVS
310 320 330 340 350
MDMLAVDLTP CPQAGIGTPV ELWGKEIKID DVAAAAGTVG YELMCALALR

VPVVTV
Length:356
Mass (Da):38,871
Last modified:November 3, 2009 - v1
Checksum:i817FAA44C8B3ED70
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP010953 Genomic DNA. Translation: BAI25002.1.
RefSeqiYP_003228742.1. NC_013361.1.

Genome annotation databases

EnsemblBacteriaiBAI25002; BAI25002; ECO26_1703.
GeneIDi8480802.
KEGGieoj:ECO26_1703.
PATRICi18398947. VBIEscCol24965_1728.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP010953 Genomic DNA. Translation: BAI25002.1.
RefSeqiYP_003228742.1. NC_013361.1.

3D structure databases

ProteinModelPortaliC8TQK2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi573235.ECO26_1703.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAI25002; BAI25002; ECO26_1703.
GeneIDi8480802.
KEGGieoj:ECO26_1703.
PATRICi18398947. VBIEscCol24965_1728.

Phylogenomic databases

eggNOGiCOG0787.
HOGENOMiHOG000031446.
KOiK01775.
OrthoDBiEOG6PP9NJ.

Enzyme and pathway databases

UniPathwayiUPA00042; UER00497.
BioCyciECOL573235:GCY7-1750-MONOMER.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_01201. Ala_racemase.
InterProiIPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR020622. Ala_racemase_pyridoxalP-BS.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]
PRINTSiPR00992. ALARACEMASE.
SMARTiSM01005. Ala_racemase_C. 1 hit.
[Graphical view]
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR00492. alr. 1 hit.
PROSITEiPS00395. ALANINE_RACEMASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Comparative genomics reveal the mechanism of the parallel evolution of O157 and non-O157 enterohemorrhagic Escherichia coli."
    Ogura Y., Ooka T., Iguchi A., Toh H., Asadulghani M., Oshima K., Kodama T., Abe H., Nakayama K., Kurokawa K., Tobe T., Hattori M., Hayashi T.
    Proc. Natl. Acad. Sci. U.S.A. 106:17939-17944(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 11368 / EHECImported.

Entry informationi

Entry nameiC8TQK2_ECO26
AccessioniPrimary (citable) accession number: C8TQK2
Entry historyi
Integrated into UniProtKB/TrEMBL: November 3, 2009
Last sequence update: November 3, 2009
Last modified: March 4, 2015
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.