C8NR43 (C8NR43_COREF) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase HAMAP MF_00193 RuleBase RU003812
EC=6.3.1.5 HAMAP MF_00193 RuleBase RU003812

Gene names

Name:	nadE HAMAP MF_00193 EMBL EEW48943.1
ORF Names:	HMPREF0290_2468 EMBL EEW48943.1

Organism

Corynebacterium efficiens YS-314 EMBL EEW48943.1

Taxonomic identifier

196164 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	280 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193 RuleBase RU003812 SAAS SAAS003694
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193 SAAS SAAS003694 RuleBase RU004252
Sequence similarities	Belongs to the NAD synthetase family. HAMAP MF_00193 RuleBase RU003811

Ontologies

Keywords
Ligand	ATP-binding HAMAP MF_00193 SAAS SAAS003694 RuleBase RU003811 NAD HAMAP MF_00193 SAAS SAAS003694 RuleBase RU003811 Nucleotide-binding
Molecular function	Ligase SAAS SAAS003694 HAMAP MF_00193 RuleBase RU003811 EMBL EEW48943.1
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	49 – 56	8	ATP By similarity HAMAP MF_00193
Sites
	Active site	51	1	By similarity HAMAP MF_00193

Sequences

	Sequence		Length	Mass (Da)
	C8NR43 [UniParc]. Last modified November 3, 2009. Version 1. Checksum: FA26C3F11E0CEC2D	FASTA	280	30,589
	10 20 30 40 50 60 MKTMTDTRDH IISQLSVVPS IEPAAEVEAR VQFLVDYLRV SHARGYVLGI SGGQDSTIAG 70 80 90 100 110 120 RLAQLAVERI RREDGSDHQF VGVRLPHGLQ ADEDDALVAL DFIQPDRSIS INIREATDVL 130 140 150 160 170 180 SAATAAALGI DELGDFNKGN VKARQRMVAQ YAIAGELGLL VVGTDHAAEN VTGFFTKYGD 190 200 210 220 230 240 GAADVLPLAG LTKRQGALLL QHLGAPESTW TKVPTADLEE DRPALPDEEA LGVTYREIDT 250 260 270 280 YLENSGEVSP EAAARIEHLW KVGQHKRHMP VTPQDQWWRQ

References

[1]

Muzny D., Qin X., Deng J., Jiang H., Liu Y., Qu J., Song X.-Z., Zhang L., Thornton R., Coyle M., Francisco L., Jackson L., Javaid M., Korchina V., Kovar C., Mata R., Mathew T., Ngo R. , Nguyen L., Nguyen N., Okwuonu G., Ongeri F., Pham C., Simmons D., Wilczek-Boney K., Hale W., Jakkamsetti A., Pham P., Ruth R., San Lucas F., Warren J., Zhang J., Zhao Z., Zhou C., Zhu D., Lee S., Bess C., Blankenburg K., Forbes L., Fu Q., Gubbala S., Hirani K., Jayaseelan J.C., Lara F., Munidasa M., Palculict T., Patil S., Pu L.-L., Saada N., Tang L., Weissenberger G., Zhu Y., Hemphill L., Shang Y., Youmans B., Ayvaz T., Ross M., Santibanez J., Aqrawi P., Gross S., Joshi V., Fowler G., Nazareth L., Reid J., Worley K., Petrosino J., Highlander S., Gibbs R. Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: YS-314 EMBL EEW48943.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	ACLI01000101 Genomic DNA. Translation: EEW48943.1.
RefSeq	NP_739038.1. NC_004369.1.
3D structure databases
ProteinModelPortal	C8NR43.
ModBase	Search...
Protein-protein interaction databases
STRING	C8NR43.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1034546.
PATRIC	21490985. VBICorEff9312_2397.
Phylogenomic databases
OMA	GLNKRRV.
ProtClustDB	PRK00768.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

C8NR43_COREF

Accession

Primary (citable) accession number: C8NR43

Entry history

Integrated into UniProtKB/TrEMBL:	November 3, 2009
Last sequence update:	November 3, 2009
Last modified:	December 14, 2011
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)