C8NK74 (C8NK74_COREF) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Amidophosphoribosyltransferase PIRNR PIRNR000485
Short name=ATase PIRNR PIRNR000485
EC=2.4.2.14 PIRNR PIRNR000485

Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase PIRNR PIRNR000485

Gene names

Name:	purF EMBL EEW51012.1
ORF Names:	HMPREF0290_0399 EMBL EEW51012.1

Organism

Corynebacterium efficiens YS-314 EMBL EEW51012.1

Taxonomic identifier

196164 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O. PIRNR PIRNR000485
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity. PIRSR PIRSR000485-3 Binds 1 magnesium ion per subunit By similarity. PIRNR PIRNR000485
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2. PIRNR PIRNR000485
Sequence similarities	In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. PIRNR PIRNR000485

Ontologies

Keywords
Biological process	Purine biosynthesis PIRNR PIRNR000485
Ligand	Iron Iron-sulfur PIRSR PIRSR000485-3 Magnesium PIRNR PIRNR000485 Metal-binding
Molecular function	Glycosyltransferase PIRNR PIRNR000485 EMBL EEW51012.1 Transferase
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amidophosphoribosyltransferase activity Inferred from electronic annotation. Source: EC iron-sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	32	1	For GATase activity By similarity PIRSR PIRSR000485-1
	Metal binding	271	1	Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
	Metal binding	417	1	Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
	Metal binding	475	1	Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
	Metal binding	478	1	Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3

Sequences

	Sequence		Length	Mass (Da)
	C8NK74 [UniParc]. Last modified November 3, 2009. Version 1. Checksum: 41020316003D0E61	FASTA	510	54,990
	10 20 30 40 50 60 MHVAQLDQHT SPGSVVNYPY DDHNEQSPQE ECGVFGVWAP GEEVAKLTYF GLFALQHRGQ 70 80 90 100 110 120 EAAGIAVGDG EQILVFKDLG LVSQVFDEPI LESLQGDIAI GHTRYTTAGG NSWENAQPMF 130 140 150 160 170 180 RMAPDGTDVA LGHNGNLINH VQLREKATEL GLVDPQQKPS DTDVLTALLA NGIGDGDDLF 190 200 210 220 230 240 ESARKLLPEV RGAYCLTFTD GHTLYAARDP YGIRPLSIGR LARGWVVASE TSALDIVGAS 250 260 270 280 290 300 LVREVEPGEL IAIDESGLRS TRFAETNRKA CVFEYVYLAR PDSVIKGRNV NEVRLEIGRT 310 320 330 340 350 360 LAREAPAEGD LVIPTPDSGT PAAVGFAQAS GIPFGQGMVK NAYVGRTFIQ PSDTLRQLGI 370 380 390 400 410 420 RLKLNPLREV IAGKRLVVVD DSIVRGNTQR AVIRMLREAG AAEVHVRIAS PPVKWPCFYG 430 440 450 460 470 480 IDFATPGELI ANAVTSDNEE EMVEAVRSAI GADSLGYVSI DGMVASTTKP VEELCLACFN 490 500 510 GHYPMGLPEA NPNAELVFKM QAGRQDKIGT

References

[1]

Muzny D., Qin X., Deng J., Jiang H., Liu Y., Qu J., Song X.-Z., Zhang L., Thornton R., Coyle M., Francisco L., Jackson L., Javaid M., Korchina V., Kovar C., Mata R., Mathew T., Ngo R. , Nguyen L., Nguyen N., Okwuonu G., Ongeri F., Pham C., Simmons D., Wilczek-Boney K., Hale W., Jakkamsetti A., Pham P., Ruth R., San Lucas F., Warren J., Zhang J., Zhao Z., Zhou C., Zhu D., Lee S., Bess C., Blankenburg K., Forbes L., Fu Q., Gubbala S., Hirani K., Jayaseelan J.C., Lara F., Munidasa M., Palculict T., Patil S., Pu L.-L., Saada N., Tang L., Weissenberger G., Zhu Y., Hemphill L., Shang Y., Youmans B., Ayvaz T., Ross M., Santibanez J., Aqrawi P., Gross S., Joshi V., Fowler G., Nazareth L., Reid J., Worley K., Petrosino J., Highlander S., Gibbs R. Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: YS-314 EMBL EEW51012.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	ACLI01000021 Genomic DNA. Translation: EEW51012.1.
RefSeq	NP_739086.1. NC_004369.1.
3D structure databases
ProteinModelPortal	C8NK74.
ModBase	Search...
Protein-protein interaction databases
STRING	C8NK74.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1032099.
PATRIC	21491099. VBICorEff9312_2444.
Phylogenomic databases
OMA	VWAPGEE.
ProtClustDB	PRK07847.
Family and domain databases
InterPro	IPR005854. Amd_phspho_trans. IPR000583. GATase_2. IPR017932. GATase_II. IPR000836. PRibTrfase. [Graphical view]
PANTHER	PTHR11907. Amd_phspho_trans. 1 hit.
Pfam	PF00310. GATase_2. 1 hit. PF00156. Pribosyltran. 1 hit. [Graphical view]
PIRSF	PIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMs	TIGR01134. PurF. 1 hit.
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C8NK74_COREF

Accession

Primary (citable) accession number: C8NK74

Entry history

Integrated into UniProtKB/TrEMBL:	November 3, 2009
Last sequence update:	November 3, 2009
Last modified:	January 25, 2012
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)