C8LAJ5 (C8LAJ5_STAAU) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
L-lactate dehydrogenase 1 HAMAP MF_00488
Short name=L-LDH 1 HAMAP MF_00488
EC=1.1.1.27 HAMAP MF_00488

Gene names

Name:	ldh1 HAMAP MF_00488
ORF Names:	SAGG_01402 EMBL EEV83656.1

Organism

Staphylococcus aureus A5948 EMBL EEV83656.1

Taxonomic identifier

553567 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-lactate + NAD+ = pyruvate + NADH. HAMAP MF_00488
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. HAMAP MF_00488
Subunit structure	Homotetramer By similarity. HAMAP MF_00488
Subcellular location	Cytoplasm By similarity HAMAP MF_00488.
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family. HAMAP MF_00488

Ontologies

Keywords
Biological process	Glycolysis HAMAP MF_00488 Stress response HAMAP MF_00488
Cellular component	Cytoplasm HAMAP MF_00488
Ligand	NAD HAMAP MF_00488
Molecular function	Oxidoreductase HAMAP MF_00488
PTM	Phosphoprotein HAMAP MF_00488
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-lactate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	14 – 42	29	NAD By similarity HAMAP MF_00488
Sites
	Active site	178	1	Proton acceptor By similarity HAMAP MF_00488
	Binding site	91	1	Substrate By similarity HAMAP MF_00488
	Binding site	123	1	NAD or substrate By similarity HAMAP MF_00488
	Binding site	154	1	Substrate By similarity HAMAP MF_00488
	Binding site	231	1	Substrate By similarity HAMAP MF_00488
Amino acid modifications
	Modified residue	222	1	Phosphotyrosine By similarity HAMAP MF_00488

Sequences

	Sequence		Length	Mass (Da)
	C8LAJ5 [UniParc]. Last modified November 3, 2009. Version 1. Checksum: 7779343BCA8006C4	FASTA	319	34,420
	10 20 30 40 50 60 MKTFGKKVVL IGDGSVGSSY AFAMVTQGVA DEFVIIDIAK DKVKADVQDL NHGTVHSPSP 70 80 90 100 110 120 VDVKAGEYED CKDADLVVIT AGAPQKPGET RLQLVEKNTK IMKSIVKSVM DSGFDGYFLI 130 140 150 160 170 180 AANPVDILTR FVKEYTGLPA ERVIGSGTVL DSARLQYLIS QELGVAPSSV DASIIGEHGD 190 200 210 220 230 240 TELAVWSQAN VAGISVYDTL KEQTGSEAKA EEIYVNTRDA AYEIIQAKGS TYYGIALALM 250 260 270 280 290 300 RISKAILNNE NNVLNVSIQL DGQYGGHKGV YLGVPTLVNQ HGAVKIYEMP LSAEEQALFD 310 KSVKTLEDTF DSIKYLLED

References

[1]

"The Genome Sequence of Staphylococcus aureus strain A5948." The Broad Institute Genome Sequencing Platform Ward D., Young S.K., Zeng Q., Koehrsen M., Godfrey P., Alvarado L., Berlin A., Borenstein D., Chen Z., Engels R., Freedman E., Gellesch M., Goldberg J., Griggs A., Gujja S., Heiman D., Hepburn T., Howarth C. , Jen D., Larson L., Lewis B., Mehta T., Park D., Pearson M., Roberts A., Saif S., Shea T., Shenoy N., Sisk P., Stolte C., Sykes S., Walk T., White J., Yandava C., Peleg A.Y., Galagan J., Nusbaum C., Birren B. Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: A5948 EMBL EEV83656.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	ACKD01000024 Genomic DNA. Translation: EEV83656.1.
3D structure databases
ProteinModelPortal	C8LAJ5.
SMR	C8LAJ5. Positions 5-313.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
PATRIC	29473735. VBIStaAur101774_1028.
Family and domain databases
HAMAP	MF_00488. Lactate_dehydrog. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR011304. L-lactate_DH. IPR018177. L-lactate_DH_AS. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11540:SF3. PTHR11540:SF3. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C8LAJ5_STAAU

Accession

Primary (citable) accession number: C8LAJ5

Entry history

Integrated into UniProtKB/TrEMBL:	November 3, 2009
Last sequence update:	November 3, 2009
Last modified:	December 14, 2011
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)