C8KML8 (C8KML8_STAAU) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
6-phospho-beta-galactosidase HAMAP MF_01574 RuleBase RU004469
EC=3.2.1.85 HAMAP MF_01574 RuleBase RU004469

Alternative name(s):
Beta-D-phosphogalactoside galactohydrolase HAMAP MF_01574
P-beta-Gal HAMAP MF_01574

Gene names

Name:	lacG HAMAP MF_01574 EMBL EEW44741.1
ORF Names:	SA930_1222 EMBL EEW44741.1

Organism

Staphylococcus aureus 930918-3 EMBL EEW44741.1

Taxonomic identifier

452948 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	470 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	A 6-phospho-beta-D-galactoside + H2O = 6-phospho-D-galactose + an alcohol. HAMAP MF_01574 RuleBase RU004469
Pathway	Carbohydrate metabolism; lactose degradation; D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate: step 1/1. HAMAP MF_01574 RuleBase RU004469
Sequence similarities	Belongs to the glycosyl hydrolase 1 family. HAMAP MF_01574 RuleBase RU003690

Ontologies

Keywords
Molecular function	Glycosidase RuleBase RU004468 HAMAP MF_01574 Hydrolase
Gene Ontology (GO)
Biological process	lactose catabolic process via tagatose-6-phosphate Inferred from electronic annotation. Source: InterPro
Molecular function	6-phospho-beta-galactosidase activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	160	1	Proton donor By similarity HAMAP MF_01574
	Active site	375	1	Nucleophile By similarity HAMAP MF_01574

Sequences

	Sequence		Length	Mass (Da)
	C8KML8 [UniParc]. Last modified November 3, 2009. Version 1. Checksum: 9A50A44B5ABFE74F	FASTA	470	54,550
	10 20 30 40 50 60 MTKTLPEDFI FGGATAAYQA EGATNTDGKG RVAWDTYLEE NYWYTAEPAS DFYNRYPVDL 70 80 90 100 110 120 ELSEKFGVNG IRISIAWSRI FPNGYGEVNP KGVEYYHKLF AECHKRHVEP FVTLHHFDTP 130 140 150 160 170 180 EVLHKDGDFL NRKTIDYFVD YAEYCFKEFP EVKYWTTFNE IGPIGDGQYL VGKFPPGIKY 190 200 210 220 230 240 DFEKVFQSHH NMMVAHARAV KLFKDGGYKG EIGVVHALPT KYPFDPSNPE DVRAAELEDI 250 260 270 280 290 300 IHNKFILDAT YLGKYSRETM EGVQHILSVN GGKLNITDED YAILDAAKDL NDFLGINYYM 310 320 330 340 350 360 SDWMRGYDGE SEITHNATGD KGGSKYQLKG VGQREFDVDV PRTDWDWMIY PQGLYDQIMR 370 380 390 400 410 420 VVKDYPNYHK IYITENGLGY KDEFIESEKT VHDDARIDYV RQHLNVIADA IIDGANVKGY 430 440 450 460 470 FIWSLMDVFS WSNGYEKRYG LFYVDFETQE RYPKKSAYWY KELAETKEIK

References

[1]	"Genome sequencing and analysis reveals possible determinants of Staphylococcus aureus nasal carriage." Sivaraman K., Venkataraman N., Tsai J., Dewell S., Cole A.M. BMC Genomics 9:433-433(2008) [PubMed: 18808706] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: 930918-3 EMBL EEW44741.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	ABFA01000025 Genomic DNA. Translation: EEW44741.1.
3D structure databases
ProteinModelPortal	C8KML8.
SMR	C8KML8. Positions 1-470.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
PATRIC	35526831. VBIStaAur16924_1572.
Family and domain databases
HAMAP	MF_01574. LacG. [Tree]
InterPro	IPR005928. 6P-beta-galactosidase. IPR001360. Glyco_hydro_1. IPR018120. Glyco_hydro_1_AS. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10353. Glyco_hydro_1. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
TIGRFAMs	TIGR01233. LacG. 1 hit.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit. PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C8KML8_STAAU

Accession

Primary (citable) accession number: C8KML8

Entry history

Integrated into UniProtKB/TrEMBL:	November 3, 2009
Last sequence update:	November 3, 2009
Last modified:	December 14, 2011
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)