C8AF96 (C8AF96_STAAU) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
1-pyrroline-5-carboxylate dehydrogenase HAMAP MF_00733
Short name=P5C dehydrogenase HAMAP MF_00733
EC=1.5.1.12 HAMAP MF_00733

Gene names

Name:	rocA HAMAP MF_00733
ORF Names:	SADG_00375 EMBL EEV13007.1

Organism

Staphylococcus aureus subsp. aureus E1410 EMBL EEV13007.1

Taxonomic identifier

585153 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	514 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-1-pyrroline-5-carboxylate + NAD(P)+ + 2 H2O = L-glutamate + NAD(P)H. HAMAP MF_00733
Pathway	Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2. HAMAP MF_00733
Sequence similarities	Belongs to the aldehyde dehydrogenase family. RuleBase RU003345 Belongs to the aldehyde dehydrogenase family. RocA subfamily. HAMAP MF_00733

Ontologies

Keywords
Ligand	NAD HAMAP MF_00733
Molecular function	Oxidoreductase HAMAP MF_00733 RuleBase RU003344
Gene Ontology (GO)
Biological process	glutamate biosynthetic process Inferred from electronic annotation. Source: HAMAP proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	1-pyrroline-5-carboxylate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	286	1	By similarity HAMAP MF_00733
	Active site	320	1	By similarity HAMAP MF_00733

Sequences

	Sequence		Length	Mass (Da)
	C8AF96 [UniParc]. Last modified October 13, 2009. Version 1. Checksum: 117DF65C22339CDB	FASTA	514	56,868
	10 20 30 40 50 60 MVVEFKNEPG YDFSVQENVD MFKKALKDVE KELGQDIPLV INGEKIFKDD KIKSINPADT 70 80 90 100 110 120 SQVIANASKA TKQDVEDAFK AANEAYKSWK TWSANDRAEL MLRVSAIIRR RKAEIAAIMV 130 140 150 160 170 180 YEAGKPWDEA VGDAAEGIDF IEYYARSMMD LAQGKPVLDR EGEHNKYFYK SIGTGVTIPP 190 200 210 220 230 240 WNFPFAIMAG TTLAPVVAGN TVLLKPAEDT PYIAYKLMEI LEEAGLPKGV VNFVPGDPKE 250 260 270 280 290 300 IGDYLVDHKD THFVTFTGSR ATGTRIYERS AVVQEGQNFL KRVIAEMGGK DAIVVDENID 310 320 330 340 350 360 TDMAAEAIVT SAFGFSGQKC SACSRAIVHK DVYDEVLEKS IKLTKELTLG NTVDNTYMGP 370 380 390 400 410 420 VINKKQFDKI KNYIEIGKEE GKLEQGGGTD DSKGYFVEPT IISGLKSKDR IMQEEIFGPV 430 440 450 460 470 480 VGFVKVNDFD EAIEVANDTD YGLTGAVITN NREHWIKAVN EFDVGNLYLN RGCTSAVVGY 490 500 510 HPFGGFKMSG TDAKTGSPDY LLHFLEQKVV SEMF

References

[1]

"The Genome Sequence of Staphylococcus aureus strain E1410." The Broad Institute Genome Sequencing Platform Feldgarden M., Robinson A., Wong A., Smyth D., Young S.K., Zeng Q., Koehrsen M., Godfrey P., Alvarado L., Berlin A., Borenstein D., Chen Z., Engels R., Freedman E., Gellesch M., Goldberg J., Griggs A., Gujja S. , Heiman D., Hepburn T., Howarth C., Jen D., Larson L., Lewis B., Mehta T., Park D., Pearson M., Roberts A., Saif S., Shea T., Shenoy N., Sisk P., Stolte C., Sykes S., Walk T., White J., Yandava C., Wirth D.F., Galagan J., Nusbaum C., Birren B. Submitted (APR-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: E1410 EMBL EEV13007.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	GG700598 Genomic DNA. Translation: EEV13007.1.
3D structure databases
ProteinModelPortal	C8AF96.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
PATRIC	26072488. VBIStaAur29047_0833.
Family and domain databases
HAMAP	MF_00733. RocA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR005932. Delta1-pyrroline-5-COlate_DH-2. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR01237. D1pyr5carbox2. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C8AF96_STAAU

Accession

Primary (citable) accession number: C8AF96

Entry history

Integrated into UniProtKB/TrEMBL:	October 13, 2009
Last sequence update:	October 13, 2009
Last modified:	December 14, 2011
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)