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Protein

Alpha-amylase

Gene

NECHADRAFT_51674

Organism
Nectria haematococca (strain 77-13-4 / ATCC MYA-4622 / FGSC 9596 / MPVI) (Fusarium solani subsp. pisi)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi134Calcium 1UniRule annotation1
Metal bindingi169Calcium 1; via carbonyl oxygenUniRule annotation1
Metal bindingi178Calcium 1UniRule annotation1
Active sitei209NucleophileUniRule annotation1
Metal bindingi209Calcium 2UniRule annotation1
Metal bindingi213Calcium 1; via carbonyl oxygenUniRule annotation1
Active sitei233Proton donorUniRule annotation1
Metal bindingi233Calcium 2UniRule annotation1
Sitei299Transition state stabilizerUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidaseUniRule annotation, Hydrolase
Biological processCarbohydrate metabolismUniRule annotation
LigandCalciumUniRule annotation, Metal-bindingUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylaseUniRule annotation (EC:3.2.1.1UniRule annotation)
Gene namesi
ORF Names:NECHADRAFT_51674Imported
OrganismiNectria haematococca (strain 77-13-4 / ATCC MYA-4622 / FGSC 9596 / MPVI) (Fusarium solani subsp. pisi)Imported
Taxonomic identifieri660122 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesNectriaceaeFusariumFusarium solani species complex
Proteomesi
  • UP000005206 Componenti: Unassembled WGS sequence

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_500298710021 – 459Alpha-amylaseSequence analysisAdd BLAST439

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi50 ↔ 57UniRule annotation
Disulfide bondi158 ↔ 171UniRule annotation

Keywords - PTMi

Disulfide bondUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi140110.NechaP51674.

Structurei

3D structure databases

ProteinModelPortaliC7ZH60.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 371AamyInterPro annotationAdd BLAST339

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.UniRule annotation

Keywords - Domaini

SignalSequence analysis

Phylogenomic databases

InParanoidiC7ZH60.
KOiK01176.
OrthoDBiEOG092C1WPY.

Family and domain databases

InterProiView protein in InterPro
IPR013777. A-amylase-like.
IPR006046. Alpha_amylase.
IPR006047. Glyco_hydro_13_cat_dom.
IPR017853. Glycoside_hydrolase_SF.
PfamiView protein in Pfam
PF00128. Alpha-amylase. 1 hit.
PIRSFiPIRSF001024. Alph-amyl_fung. 1 hit.
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiView protein in SMART
SM00642. Aamy. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

C7ZH60-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPLYALAAV FASICPTALA ADAAAWKSRN IYFVLTDRIA RSSTDNGGAC
60 70 80 90 100
SDLGSYCGGT FKGLESKLDY IRGMGFDAIW ITPVVSNTPN GYHGYWADDL
110 120 130 140 150
YAINSKYGTA EDLKSLVSSA HQKGIYVMVD VVANHMGPNI GGHKPEPLNQ
160 170 180 190 200
QSSYHSGCEI NYSDQTSIEV CSIAGLPDVK TENSEIRTLY QNWIKWLVKE
210 220 230 240 250
YQFDGIRIDT VKHVEKDFWP GFQSAAGVYS IGEVFDGNPN YLAGYASVMP
260 270 280 290 300
GLLNYAIYYP MNRFYQQQGS SQDLVNMHDE ISAKFSDPTV LGTFLDNHDN
310 320 330 340 350
ARWLNRKNDV SLLKNALAHV ILARGIPIVY YGTEQGYAGG NDPANREDLW
360 370 380 390 400
RSGFSTNADL YQAISRLSNA RSKAGGLGGN DHKHLFVENN VYAWSRAGGD
410 420 430 440 450
LVVLTTNRGQ GWSGQYCFFT QKPNKSWNNV FGQGSYTSDN DGRVCVNVTN

GEPVVLLAS
Length:459
Mass (Da):50,513
Last modified:October 13, 2009 - v1
Checksum:i760D313E929AF4AE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GG698927 Genomic DNA. Translation: EEU36582.1.
RefSeqiXP_003042295.1. XM_003042249.1.

Genome annotation databases

EnsemblFungiiNechaT51674; NechaP51674; NechaG51674.
GeneIDi9669783.
KEGGinhe:NECHADRAFT_51674.

Similar proteinsi

Entry informationi

Entry nameiC7ZH60_NECH7
AccessioniPrimary (citable) accession number: C7ZH60
Entry historyiIntegrated into UniProtKB/TrEMBL: October 13, 2009
Last sequence update: October 13, 2009
Last modified: October 25, 2017
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported