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C7Z527

- LIPA_NECH7

UniProt

C7Z527 - LIPA_NECH7

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Protein
Lipoyl synthase, mitochondrial
Gene
NECHADRAFT_66689
Organism
Nectria haematococca (strain 77-13-4 / ATCC MYA-4622 / FGSC 9596 / MPVI) (Fusarium solani subsp. pisi)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi124 – 1241Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi129 – 1291Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi135 – 1351Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi155 – 1551Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi159 – 1591Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi162 – 1621Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrial (EC:2.8.1.8)
Alternative name(s):
Lipoate synthase
Short name:
LS
Short name:
Lip-syn
Lipoic acid synthase
Gene namesi
ORF Names:NECHADRAFT_66689
OrganismiNectria haematococca (strain 77-13-4 / ATCC MYA-4622 / FGSC 9596 / MPVI) (Fusarium solani subsp. pisi)
Taxonomic identifieri660122 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesNectriaceaeFusariumFusarium solani species complex
ProteomesiUP000005206: Unassembled WGS sequence

Subcellular locationi

Mitochondrion Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2828Mitochondrion Reviewed prediction
Add
BLAST
Chaini29 – 412384Lipoyl synthase, mitochondrialUniRule annotation
PRO_0000398273Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliC7Z527.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

KOiK03644.
OrthoDBiEOG79KPR7.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C7Z527-1 [UniParc]FASTAAdd to Basket

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MASIAPSLKR AHAPLRKALT ASSTIRAFAT VPPTSSETTK PKRKSYFKDT    50
TVAEFSDFLA TSSPAQPLSP AEAFTLRTAE VGPEGKKRTI TRLPEWLKTP 100
IPAGNDNFKS IKKDLRGLGL HTVCEEARCP NISECWGGSD KNAATATIML 150
MGDTCTRGCR FCSVKTNRKP AALDPHEPEN TAEALARWGL GYVVLTSVDR 200
DDLADGGAHH FAETIRRIKQ KKPSLLVEAL TGDFRGDLDM VKVVAESGLD 250
VYAHNVETVE DLTPYVRDRR ATFRQSLSVL KHVKEVKGKE GIITKTSLML 300
GLGEQEHEVM AALEDLRKAD VDVVTFGQYM RPTKRHLKVE KYVTPDEFEM 350
WNKRALDMGF LYCASGPLVR SSYKAGEAFI ENVLRKRSGE KAMARGTLAK 400
AVALDSETRS SI 412
Length:412
Mass (Da):45,389
Last modified:October 13, 2009 - v1
Checksum:i18E930BB46989CFE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
GG698910 Genomic DNA. Translation: EEU40447.1.
RefSeqiXP_003046160.1. XM_003046114.1.

Genome annotation databases

EnsemblFungiiNechaT66689; NechaP66689; NechaG66689.
GeneIDi9678266.
KEGGinhe:NECHADRAFT_66689.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
GG698910 Genomic DNA. Translation: EEU40447.1 .
RefSeqi XP_003046160.1. XM_003046114.1.

3D structure databases

ProteinModelPortali C7Z527.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii NechaT66689 ; NechaP66689 ; NechaG66689 .
GeneIDi 9678266.
KEGGi nhe:NECHADRAFT_66689.

Phylogenomic databases

KOi K03644.
OrthoDBi EOG79KPR7.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 77-13-4 / ATCC MYA-4622 / FGSC 9596 / MPVI.

Entry informationi

Entry nameiLIPA_NECH7
AccessioniPrimary (citable) accession number: C7Z527
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: October 13, 2009
Last modified: June 11, 2014
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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