C7YYG9 (DAPB_NECH7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable dipeptidyl-aminopeptidase B
Short name=DPAP B
EC=3.4.14.5

Gene names

Name:	DAPB
ORF Names:	NECHADRAFT_47100

Organism

Nectria haematococca (strain 77-13-4 / ATCC MYA-4622 / FGSC 9596 / MPVI) (Fusarium solani subsp. pisi) [Reference proteome]

Taxonomic identifier

660122 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Sordariomycetes › Hypocreomycetidae › Hypocreales › Nectriaceae › Nectria › Nectria haematococca complex › Nectria haematococca › Fusarium solani subsp. pisi

Protein attributes

Sequence length	912 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Type IV dipeptidyl-peptidase which removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline By similarity.
Catalytic activity	Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.
Subcellular location	Vacuole membrane; Single-pass type II membrane protein By similarity. Note: Lysosome-like vacuoles By similarity.
Sequence similarities	Belongs to the peptidase S9B family.

Ontologies

Keywords
Cellular component	Membrane Vacuole
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Aminopeptidase Hydrolase Protease Serine protease
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW vacuolar membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW serine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 912	912	Probable dipeptidyl-aminopeptidase B		PRO_0000412150
Regions
	Topological domain	1 – 85	85	Cytoplasmic Potential
	Transmembrane	86 – 106	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	107 – 912	806	Vacuolar Potential
Sites
	Active site	749	1	Charge relay system By similarity
	Active site	826	1	Charge relay system By similarity
	Active site	859	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	344	1	N-linked (GlcNAc...) Potential
	Glycosylation	808	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	C7YYG9 [UniParc]. Last modified October 13, 2009. Version 1. Checksum: 316574CA966658A3	FASTA	912	102,536
	10 20 30 40 50 60 MSSALSPEGD RRYSDDSLSS VSTTSLVFER IQEKTEMDAD NDKEKDPRAL DDEDPLRDED 70 80 90 100 110 120 DLETGPFLGP GASLHREPMD RGLRRILIIV AVVFIGGWLA GLGIFIASGS YHHESDTEHD 130 140 150 160 170 180 PDANSRGSGK SLSMDQLFDG TWSPKYHSIS WIAGPKGEDG LLLEVGASNK PYIVVEDIRS 190 200 210 220 230 240 DKNVATRDDA EPKASNSRTL MEHPYFEYDG KQYSPSWSEP SPDLTKVLLG VDRKKNWRHS 250 260 270 280 290 300 FSAIYFVLDV KTQEAEPLVP DQVDARIQLA SWSPKSDAVS FTRENNLYIR RLTGDKDVTQ 310 320 330 340 350 360 ITKDGGPEYF YGIPDWVYEE EVFSGRSATW WSDDGKYLAF LRTNETGVPE YPVQFFIERP 370 380 390 400 410 420 SGTTPEDGEE AYPEVEQIKY PKAGAHNPVV DLQFYDIGKK DTFSVEIDGA FADDDRIINN 430 440 450 460 470 480 LLWAGDKAIV KQTNRVSDVL KVVLVDVPSR KGKTINTINI NEIDGGWFEI SHKMTYIPAD 490 500 510 520 530 540 PKNGREHDGY VDSVIHEGYD HLAYFTPLDN SEPIMLTKGN WEVDDAPSAV DLANNLVYFI 550 560 570 580 590 600 AAKESSIQRH VYSVKLDGSD LQALTDPKTE AYYDASFSKG AGFVFLSYRG PKVPTQKVIS 610 620 630 640 650 660 TPVSASSYER IIEDNAELAD RARRHELPIL KYGTLDLDTG VKVNYVERRP PHFDAKKQYP 670 680 690 700 710 720 VLFHQYSGPG SQSVTKRFAV DFQAYVAAAL GYLVITVDPR GTGFLGRKHR VTVRSKLGVH 730 740 750 760 770 780 EAHDHIAAAA SFASRPYVDA ERLAIWGWSY GGFTTLKTLE QDAGRTFSYG MAVAPVTDWR 790 800 810 820 830 840 FYDSIYTERY MRTPQDNPDG YDLSKVANAT ALGENKRFLL MHGVADDNVH FQNSLTLLDD 850 860 870 880 890 900 LDLAGVENYD VHVFPDSDHS IYFHNGNRIV YDKLRNWLIN AFNGEWLKVS NPQPQKDPVE 910 KEKRHMVPQA LV

References

[1]

"The genome of Nectria haematococca: contribution of supernumerary chromosomes to gene expansion." Coleman J.J., Rounsley S.D., Rodriguez-Carres M., Kuo A., Wasmann C.C., Grimwood J., Schmutz J., Taga M., White G.J., Zhou S., Schwartz D.C., Freitag M., Ma L.-J., Danchin E.G.J., Henrissat B., Coutinho P.M., Nelson D.R., Straney D. , Napoli C.A., Barker B.M., Gribskov M., Rep M., Kroken S., Molnar I., Rensing C., Kennell J.C., Zamora J., Farman M.L., Selker E.U., Salamov A., Shapiro H., Pangilinan J., Lindquist E., Lamers C., Grigoriev I.V., Geiser D.M., Covert S.F., Temporini E., VanEtten H.D. PLoS Genet. 5:E1000618-E1000618(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 77-13-4 / ATCC MYA-4622 / FGSC 9596 / MPVI.

Cross-references

Sequence databases
EMBL GenBank DDBJ	GG698903 Genomic DNA. Translation: EEU43005.1.
RefSeq	XP_003048718.1. XM_003048672.1.
3D structure databases
ProteinModelPortal	C7YYG9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	NechaT47100; NechaP47100; NechaG47100.
GeneID	9665490.
KEGG	nhe:NECHADRAFT_47100.
Phylogenomic databases
KO	K01282.
OrthoDB	EOG4Q885J.
Family and domain databases
InterPro	IPR001375. Peptidase_S9. IPR002469. Peptidase_S9B. [Graphical view]
Pfam	PF00930. DPPIV_N. 1 hit. PF00326. Peptidase_S9. 1 hit. [Graphical view]
PROSITE	PS00708. PRO_ENDOPEP_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPB_NECH7

Accession

Primary (citable) accession number: C7YYG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 27, 2011
Last sequence update:	October 13, 2009
Last modified:	October 3, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program