C7YVN8 (AMPP3_NECH7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable Xaa-Pro aminopeptidase PEPP
EC=3.4.11.9

Alternative name(s):
Aminoacylproline aminopeptidase
Prolidase

Gene names

Name:	PEPP
ORF Names:	NECHADRAFT_74024

Organism

Nectria haematococca (strain 77-13-4 / ATCC MYA-4622 / FGSC 9596 / MPVI) (Fusarium solani subsp. pisi) [Reference proteome]

Taxonomic identifier

660122 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Sordariomycetes › Hypocreomycetidae › Hypocreales › Nectriaceae › Nectria › Nectria haematococca complex › Nectria haematococca › Fusarium solani subsp. pisi

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides By similarity.
Catalytic activity	Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.
Cofactor	Binds 2 manganese ions per subunit By similarity.
Sequence similarities	Belongs to the peptidase M24B family.

Ontologies

Keywords
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Metalloprotease Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	Probable Xaa-Pro aminopeptidase PEPP		PRO_0000411877
Sites
	Metal binding	257	1	Manganese 2 By similarity
	Metal binding	268	1	Manganese 1 By similarity
	Metal binding	268	1	Manganese 2 By similarity
	Metal binding	391	1	Manganese 1 By similarity
	Metal binding	436	1	Manganese 1 By similarity
	Metal binding	436	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	C7YVN8 [UniParc]. Last modified October 13, 2009. Version 1. Checksum: F0FD86FB52B4496C	FASTA	469	52,429
	10 20 30 40 50 60 MFAEDLDRIL SGKYPGKSHA LRVVELLREK VPNAKGYLYL EGRMSKLLED SDEFEPFRQR 70 80 90 100 110 120 RHFYYLTGCD LSNCYLLYDI DSSKSTLFIP PIDPEEVVWS GLPLSPQQGL EKYDVDEVKF 130 140 150 160 170 180 STELDNILSH LSGSQESTVY TIADQVCPHI KFGLDNVDSS ILKGVIDRCR VVKDKYEVAM 190 200 210 220 230 240 IRKANNISSL GHEAITKQAS KASNEMQLEA TFLGHCVAHG AKKMAYPPIV AAGRSGAILH 250 260 270 280 290 300 YEANDQPLGG KQNLLVDAGA EWNNYASDIT RTFPLSGTFT KESRQIYDIV YKMQMECIAI 310 320 330 340 350 360 IKAGVRWEDV HMLAHEIAVE GLLQLGIFQG AKADILKAQT SLAFFPHGLG HYLGLDTHDV 370 380 390 400 410 420 GGNPNFDDEN KYLRYLRTRG TLPAGSVVTV EPGIYFCEHI IRPYLQDERH KDLINSDVLD 430 440 450 460 KYWDVGGIRL TQGSIEDNVL VTPTGVDNLT TTIKHPDQLE GMIRGLEGV

References

[1]

"The genome of Nectria haematococca: contribution of supernumerary chromosomes to gene expansion." Coleman J.J., Rounsley S.D., Rodriguez-Carres M., Kuo A., Wasmann C.C., Grimwood J., Schmutz J., Taga M., White G.J., Zhou S., Schwartz D.C., Freitag M., Ma L.-J., Danchin E.G.J., Henrissat B., Coutinho P.M., Nelson D.R., Straney D. , Napoli C.A., Barker B.M., Gribskov M., Rep M., Kroken S., Molnar I., Rensing C., Kennell J.C., Zamora J., Farman M.L., Selker E.U., Salamov A., Shapiro H., Pangilinan J., Lindquist E., Lamers C., Grigoriev I.V., Geiser D.M., Covert S.F., Temporini E., VanEtten H.D. PLoS Genet. 5:E1000618-E1000618(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 77-13-4 / ATCC MYA-4622 / FGSC 9596 / MPVI.

Cross-references

Sequence databases
EMBL GenBank DDBJ	GG698901 Genomic DNA. Translation: EEU43824.1.
RefSeq	XP_003049537.1. XM_003049491.1.
3D structure databases
ProteinModelPortal	C7YVN8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	NechaT74024; NechaP74024; NechaG74024.
GeneID	9677112.
KEGG	nhe:NECHADRAFT_74024.
Phylogenomic databases
OrthoDB	EOG4J40RD.
Family and domain databases
Gene3D	3.90.230.10. 1 hit.
InterPro	IPR007865. Aminopep_P_N. IPR000994. Pept_M24_structural-domain. [Graphical view]
Pfam	PF05195. AMP_N. 1 hit. PF00557. Peptidase_M24. 1 hit. [Graphical view]
SMART	SM01011. AMP_N. 1 hit. [Graphical view]
SUPFAM	SSF55920. Peptidase_M24_cat_core. 1 hit.
PROSITE	PS00491. PROLINE_PEPTIDASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPP3_NECH7

Accession

Primary (citable) accession number: C7YVN8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 27, 2011
Last sequence update:	October 13, 2009
Last modified:	March 6, 2013
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program