C7YSA6 (C7YSA6_NECH7) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Isocitrate dehydrogenase [NADP] PIRNR PIRNR000108
EC=1.1.1.42 PIRNR PIRNR000108

Gene names

ORF Names:

NECHADRAFT_100651 EMBL EEU45600.1

Organism

Nectria haematococca (strain 77-13-4 / ATCC MYA-4622 / FGSC 9596 / MPVI) (Fusarium solani subsp. pisi) [Reference proteome]

Taxonomic identifier

660122 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Sordariomycetes › Hypocreomycetidae › Hypocreales › Nectriaceae › Nectria › Nectria haematococca complex › Nectria haematococca › Fusarium solani subsp. pisi

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH. PIRNR PIRNR000108
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity. PIRNR PIRNR000108 PIRSR PIRSR000108-3
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family. PIRNR PIRNR000108 RuleBase RU004443

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle PIRNR PIRNR000108
Ligand	Magnesium PIRNR PIRNR000108 PIRSR PIRSR000108-3 Manganese PIRNR PIRNR000108 PIRSR PIRSR000108-3 Metal-binding PIRNR PIRNR000108 PIRSR PIRSR000108-3 NADP PIRNR PIRNR000108 PIRSR PIRSR000108-4
Molecular function	Oxidoreductase PIRNR PIRNR000108 RuleBase RU004444
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	isocitrate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	NAD binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	131 – 133	3	NADP By similarity PIRSR PIRSR000108-4
	Nucleotide binding	365 – 370	6	NADP By similarity PIRSR PIRSR000108-4
	Region	150 – 156	7	Substrate binding By similarity PIRSR PIRSR000108-2
Sites
	Metal binding	307	1	Magnesium or manganese By similarity PIRSR PIRSR000108-3
	Metal binding	330	1	Magnesium or manganese By similarity PIRSR PIRSR000108-3
	Binding site	133	1	Substrate By similarity PIRSR PIRSR000108-2
	Binding site	138	1	NADP By similarity PIRSR PIRSR000108-4
	Binding site	165	1	Substrate By similarity PIRSR PIRSR000108-2
	Binding site	188	1	Substrate By similarity PIRSR PIRSR000108-2
	Binding site	315	1	NADP By similarity PIRSR PIRSR000108-4
	Binding site	383	1	NADP; via amide nitrogen and carbonyl oxygen By similarity PIRSR PIRSR000108-4
	Site	195	1	Critical for catalysis By similarity PIRSR PIRSR000108-1
	Site	267	1	Critical for catalysis By similarity PIRSR PIRSR000108-1

Sequences

	Sequence		Length	Mass (Da)
	C7YSA6 [UniParc]. Last modified October 13, 2009. Version 1. Checksum: A7923FEAA03157C7	FASTA	466	52,027
	10 20 30 40 50 60 MPAVAFASAV PRSLLSSSLR RVAFASSSPI AVRPIRASFG AVQTISPFAV RTMASAIPKI 70 80 90 100 110 120 KVKNPVVELD GDEMTRIIWQ SIKDKFIYPY LDIDLKYYDL GLEYRDETND QVTIDAAEAI 130 140 150 160 170 180 KKYQVGVKCA TITPDEARVE EFKLKQMWLS PNGTIRNALG GTVFREPIVI PRIPRLVPGW 190 200 210 220 230 240 KKPIIIGRHA FGDQYRAKDA VLPGPGKLSM VYTPEGGEPQ EIEVYQFKNG GGVAQTQYNT 250 260 270 280 290 300 DESITGFAHA SFKLALDKGL PLYMSTKNTI LKKYDGRFKD IFQELYDTQY KPEFEAKKIW 310 320 330 340 350 360 YEHRLIDDMV AQMVKSSGGY IMALKNYDGD VQSDIVAQGF GSLGLMTSVL ITPDGKTFES 370 380 390 400 410 420 EAAHGTVTRH YREHQKGNET STNPIASIFA WTRGLIQRGK LDDQPEVVSF AESLEQACID 430 440 450 460 TVDIDGVMTK DLALACGKTG REDYVTTTEY LNAVERRMKN ILKEKL

References

[1]

"The genome of Nectria haematococca: contribution of supernumerary chromosomes to gene expansion." Coleman J.J., Rounsley S.D., Rodriguez-Carres M., Kuo A., Wasmann C.C., Grimwood J., Schmutz J., Taga M., White G.J., Zhou S., Schwartz D.C., Freitag M., Ma L.-J., Danchin E.G.J., Henrissat B., Coutinho P.M., Nelson D.R., Straney D. , Napoli C.A., Barker B.M., Gribskov M., Rep M., Kroken S., Molnar I., Rensing C., Kennell J.C., Zamora J., Farman M.L., Selker E.U., Salamov A., Shapiro H., Pangilinan J., Lindquist E., Lamers C., Grigoriev I.V., Geiser D.M., Covert S.F., Temporini E., VanEtten H.D. PLoS Genet. 5:E1000618-E1000618(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 77-13-4 / ATCC MYA-4622 / FGSC 9596 / MPVI.

Cross-references

Sequence databases
EMBL GenBank DDBJ	GG698899 Genomic DNA. Translation: EEU45600.1.
RefSeq	XP_003051313.1. XM_003051267.1.
3D structure databases
ProteinModelPortal	C7YSA6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	NechaT100651; NechaP100651; NechaG100651.
GeneID	9666174.
KEGG	nhe:NECHADRAFT_100651.
Phylogenomic databases
KO	K00031.
OrthoDB	EOG47M56G.
Family and domain databases
Gene3D	3.40.718.10. 1 hit.
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR004790. Isocitrate_DH_NADP. IPR024084. IsoPropMal-DH-like_dom. [Graphical view]
PANTHER	PTHR11822. PTHR11822. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000108. IDH_NADP. 1 hit.
TIGRFAMs	TIGR00127. nadp_idh_euk. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C7YSA6_NECH7

Accession

Primary (citable) accession number: C7YSA6

Entry history

Integrated into UniProtKB/TrEMBL:	October 13, 2009
Last sequence update:	October 13, 2009
Last modified:	March 6, 2013
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)