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Protein

Arylacetonitrilase

Gene

NECHADRAFT_80653

Organism
Nectria haematococca (strain 77-13-4 / ATCC MYA-4622 / FGSC 9596 / MPVI) (Fusarium solani subsp. pisi)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Nitrilase that hydrolyzes preferentially phenylacetonitrile, (R,S)-mandelonitrile, and 3-indolylacetonitrile.2 Publications

Catalytic activityi

A nitrile + 2 H2O = a carboxylate + NH3.2 Publications
4-chlorophenylacetonitrile + 2 H2O = 4-chlorophenylacetate + NH3.2 Publications

Kineticsi

  1. KM=8.3 mM for phenylacetonitrile1 Publication
  2. KM=9.9 mM for (R,S)-mandelonitrile1 Publication
  1. Vmax=111.1 µmol/min/mg enzyme toward phenylacetonitrile1 Publication
  2. Vmax=31.3 µmol/min/mg enzyme toward (R,S)-mandelonitrile1 Publication

pH dependencei

Optimum pH is 5.5-8.5.1 Publication

Temperature dependencei

Optimum temperature is 40 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei45Proton acceptorPROSITE-ProRule annotation1
Active sitei126PROSITE-ProRule annotation1
Active sitei167NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Arylacetonitrilase2 Publications (EC:3.5.5.12 Publications, EC:3.5.5.52 Publications)
Gene namesi
ORF Names:NECHADRAFT_80653
OrganismiNectria haematococca (strain 77-13-4 / ATCC MYA-4622 / FGSC 9596 / MPVI) (Fusarium solani subsp. pisi)
Taxonomic identifieri660122 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesNectriaceaeFusariumFusarium solani species complex
Proteomesi
  • UP000005206 Componenti: Unassembled WGS sequence

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004321721 – 339ArylacetonitrilaseAdd BLAST339

Interactioni

Protein-protein interaction databases

STRINGi140110.NechaP80653.

Structurei

3D structure databases

ProteinModelPortaliC7YS90.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 290CN hydrolasePROSITE-ProRule annotationAdd BLAST286

Sequence similaritiesi

Phylogenomic databases

InParanoidiC7YS90.
OrthoDBiEOG092C351H.

Family and domain databases

Gene3Di3.60.110.10. 1 hit.
InterProiView protein in InterPro
IPR003010. C-N_Hydrolase.
IPR036526. C-N_Hydrolase_sf.
IPR000132. Nitrilase/CN_hydratase_CS.
PfamiView protein in Pfam
PF00795. CN_hydrolase. 1 hit.
SUPFAMiSSF56317. SSF56317. 1 hit.
PROSITEiView protein in PROSITE
PS50263. CN_HYDROLASE. 1 hit.
PS00920. NITRIL_CHT_1. 1 hit.

Sequencei

Sequence statusi: Complete.

C7YS90-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MACPIRVAVT QAEPVYLDLA ASVKKACGLI AEAAQNGAKL VAFSECWLPG
60 70 80 90 100
YPAWIWARPV DFELQTRYIY NSLPIESEAM ELVKATAKEH SIAVALGFSE
110 120 130 140 150
QSPSHSIYIS QAIISPQGEV VMHRRKIKPT HMERTLFGDG SGADLNNVVE
160 170 180 190 200
VDFGAEHGKI KVGCFACWEH TQPLLKYHSI SQGEAIHISM WPPIDPSAGV
210 220 230 240 250
DHPGLWSMTA DGCQNLSQTY AIESTAYVLH STSVCTQKGI ETLKTQDGLS
260 270 280 290 300
CRQPGGGHSC VIGPDGRRLT APLGDGSPDA EGIVYADLDL TKVVATRGFL
310 320 330
DIVGHYSRPD LLWLGVDREQ KENIIAKQHK AAEQEAVQG
Length:339
Mass (Da):36,773
Last modified:October 13, 2009 - v1
Checksum:i5CE9EA7B4E06CFFD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GG698899 Genomic DNA. Translation: EEU45207.1.
RefSeqiXP_003050920.1. XM_003050874.1.

Genome annotation databases

EnsemblFungiiNechaT80653; NechaP80653; NechaG80653.
GeneIDi9666163.
KEGGinhe:NECHADRAFT_80653.

Entry informationi

Entry nameiNIT_NECH7
AccessioniPrimary (citable) accession number: C7YS90
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 4, 2015
Last sequence update: October 13, 2009
Last modified: October 25, 2017
This is version 38 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families