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C7T8V0 (C7T8V0_LACRG) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddlA EMBL CAR86043.1
Synonyms:ddl HAMAP-Rule MF_00047
Ordered Locus Names:LGG_00148 EMBL CAR86043.1, LRHM_0148 EMBL BAI40675.1
OrganismLactobacillus rhamnosus (strain ATCC 53103 / GG) [Complete proteome] [HAMAP]
Taxonomic identifier568703 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length360 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. SAAS SAAS005905 HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. SAAS SAAS005905 HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. SAAS SAAS005905 HAMAP-Rule MF_00047

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. SAAS SAAS005905 HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity SAAS SAAS005905 HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain145 – 339195ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding172 – 22554ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2941Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3061Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3061Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3081Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
C7T8V0 [UniParc].

Last modified October 13, 2009. Version 1.
Checksum: 36583BD6C82AADA7

FASTA36039,261
        10         20         30         40         50         60 
MKIVVLAGGR STERNVSISS GYRITNALRQ KGQQATFIDL FLGYDLEGKT VDQVFDDANT 

        70         80         90        100        110        120 
SKDLKISDAI LTDADINKLR IDGSTQLFGP NVLAICKAAD IVFLALHGGD GENGKVQATL 

       130        140        150        160        170        180 
DLNNVKYTGS GPLASGITMN KVFSKEVMLY HGIQTAAFKE FKRNQNGKHT VPFDFPVVVK 

       190        200        210        220        230        240 
PTSGGSSVGT HIAHNQEELE NGIADVFRFD NSAIVEEFTP GREFSLGVVN GRAFSAIEIK 

       250        260        270        280        290        300 
VHSGWYDFKH KFQAGYTDFI TPPKNLDESV HQAMKDVSLQ TMDVLGLQNY GRIDFFANDK 

       310        320        330        340        350        360 
GVWVIEANNL PGMTPLSLLP QEAEADGVEY GDLVMDIING KLKLYAEGMT EAGIMTTANH 

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence of the probiotic Lactobacillus rhamnosus ATCC 53103."
Morita H., Toh H., Oshima K., Murakami M., Taylor T.D., Igimi S., Hattori M.
J. Bacteriol. 191:7630-7631(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 53103 EMBL BAI40675.1 and ATCC 53103 / GG [Tokyo].
[2]"Comparative genomic analysis of Lactobacillus rhamnosus GG reveals pili containing a human- mucus binding protein."
Kankainen M., Paulin L., Tynkkynen S., von Ossowski I., Reunanen J., Partanen P., Satokari R., Vesterlund S., Hendrickx A.P., Lebeer S., De Keersmaecker S.C., Vanderleyden J., Hamalainen T., Laukkanen S., Salovuori N., Ritari J., Alatalo E., Korpela R. expand/collapse author list , Mattila-Sandholm T., Lassig A., Hatakka K., Kinnunen K.T., Karjalainen H., Saxelin M., Laakso K., Surakka A., Palva A., Salusjarvi T., Auvinen P., de Vos W.M.
Proc. Natl. Acad. Sci. U.S.A. 106:17193-17198(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 53103 / GG [Helsinki].
[3]"Genomic Adaptation of the Lactobacillus casei Group."
Toh H., Oshima K., Nakano A., Takahata M., Murakami M., Takaki T., Nishiyama H., Igimi S., Hattori M., Morita H.
PLoS ONE 8:e75073-e75073(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: ATCC 53103 EMBL BAI40675.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP011548 Genomic DNA. Translation: BAI40675.1.
FM179322 Genomic DNA. Translation: CAR86043.1.
RefSeqYP_003169894.1. NC_013198.1.
YP_005864640.1. NC_017482.1.

3D structure databases

ProteinModelPortalC7T8V0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING568703.LGG_00148.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAI40675; BAI40675; LRHM_0148.
CAR86043; CAR86043; LGG_00148.
GeneID12473223.
8420513.
KEGGlrg:LRHM_0148.
lrh:LGG_00148.
PATRIC22261628. VBILacRha2892_0141.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMATPDWVAL.
OrthoDBEOG6ND0KB.
ProtClustDBCLSK770750.

Enzyme and pathway databases

BioCycLRHA568703:GCGS-148-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC7T8V0_LACRG
AccessionPrimary (citable) accession number: C7T8V0
Secondary accession number(s): C8UYQ5
Entry history
Integrated into UniProtKB/TrEMBL: October 13, 2009
Last sequence update: October 13, 2009
Last modified: February 19, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)