D-alanine--D-alanine ligase - Lactobacillus rhamnosus (strain ATCC 53103 / GG)

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C7T8V0 (C7T8V0_LACRG) Unreviewed, UniProtKB/TrEMBL

Last modified May 29, 2013. Version 31. History...

Clusters with 100%, 90%, 50% identity |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info Customize order

Names and origin

Protein names

Recommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047
EC=6.3.2.4 HAMAP-Rule MF_00047

Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047

Gene names

Name:	ddlA EMBL CAR86043.1
Synonyms:	ddl HAMAP-Rule MF_00047
Ordered Locus Names:	LGG_00148, LRHM_0148 EMBL BAI40675.1

Organism

Lactobacillus rhamnosus (strain ATCC 53103 / GG) [Complete proteome] [HAMAP]

Taxonomic identifier

568703 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lactobacillus ›

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. SAAS SAAS000291 HAMAP-Rule MF_00047
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. SAAS SAAS000291 HAMAP-Rule MF_00047
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity. SAAS SAAS000291 HAMAP-Rule MF_00047
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. SAAS SAAS000291 HAMAP-Rule MF_00047
Subcellular location	Cytoplasm By similarity SAAS SAAS000291 HAMAP-Rule MF_00047.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047 Contains 1 ATP-grasp domain. SAAS SAAS000291 HAMAP-Rule MF_00047

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation SAAS SAAS000291 HAMAP-Rule MF_00047 Peptidoglycan synthesis SAAS SAAS000291 HAMAP-Rule MF_00047
Cellular component	Cytoplasm SAAS SAAS000291 HAMAP-Rule MF_00047
Ligand	ATP-binding SAAS SAAS000291 HAMAP-Rule MF_00047 Magnesium SAAS SAAS000291 HAMAP-Rule MF_00047 Manganese SAAS SAAS000291 HAMAP-Rule MF_00047 Metal-binding SAAS SAAS000291 HAMAP-Rule MF_00047 Nucleotide-binding
Molecular function	Ligase SAAS SAAS000291 HAMAP-Rule MF_00047 EMBL CAR86043.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Regions

Domain

145 – 339

195

ATP-grasp By similarity HAMAP-Rule MF_00047

Nucleotide binding

172 – 225

ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding

294

Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047

Metal binding

306

Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047

Metal binding

306

Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Metal binding

308

Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence

Length

Mass (Da)

Tools

C7T8V0 [UniParc].

Last modified October 13, 2009. Version 1.
Checksum: 36583BD6C82AADA7
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FASTA

360

39,261

        10         20         30         40         50         60 
MKIVVLAGGR STERNVSISS GYRITNALRQ KGQQATFIDL FLGYDLEGKT VDQVFDDANT 

        70         80         90        100        110        120 
SKDLKISDAI LTDADINKLR IDGSTQLFGP NVLAICKAAD IVFLALHGGD GENGKVQATL 

       130        140        150        160        170        180 
DLNNVKYTGS GPLASGITMN KVFSKEVMLY HGIQTAAFKE FKRNQNGKHT VPFDFPVVVK 

       190        200        210        220        230        240 
PTSGGSSVGT HIAHNQEELE NGIADVFRFD NSAIVEEFTP GREFSLGVVN GRAFSAIEIK 

       250        260        270        280        290        300 
VHSGWYDFKH KFQAGYTDFI TPPKNLDESV HQAMKDVSLQ TMDVLGLQNY GRIDFFANDK 

       310        320        330        340        350        360 
GVWVIEANNL PGMTPLSLLP QEAEADGVEY GDLVMDIING KLKLYAEGMT EAGIMTTANH

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References

[1]

"Complete genome sequence of the probiotic Lactobacillus rhamnosus ATCC 53103."
Morita H., Toh H., Oshima K., Murakami M., Taylor T.D., Igimi S., Hattori M.
J. Bacteriol. 191:7630-7631(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 53103 EMBL BAI40675.1 and ATCC 53103 / GG [Tokyo].

[2]

"Comparative genomic analysis of Lactobacillus rhamnosus GG reveals pili containing a human- mucus binding protein."
Kankainen M., Paulin L., Tynkkynen S., von Ossowski I., Reunanen J., Partanen P., Satokari R., Vesterlund S., Hendrickx A.P.A., Lebeer S., De Keersmaecker S.C.J., Vanderleyden J., Hamalinen T., Laukkanen S., Salovuori N., Ritari J., Alatalo E., Korpela R.

de Vos W.M.
Proc. Natl. Acad. Sci. U.S.A. 106:17193-17198(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 53103 / GG [Helsinki].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP011548 Genomic DNA. Translation: BAI40675.1. FM179322 Genomic DNA. Translation: CAR86043.1.
RefSeq	YP_003169894.1. NC_013198.1. YP_005864640.1. NC_017482.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	568703.LGG_00148.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAI40675; BAI40675; LRHM_0148. CAR86043; CAR86043; LGG_00148.
GeneID	12473223. 8420513.
KEGG	lrg:LRHM_0148. lrh:LGG_00148.
PATRIC	22261628. VBILacRha2892_0141.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1181.
HOGENOM	HOG000011592.
KO	K01921.
OMA	VEDASCD.
ProtClustDB	CLSK770750.
Enzyme and pathway databases
BioCyc	LRHA568703:GCGS-148-MONOMER.
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 2 hits. 3.40.50.20. 1 hit.
HAMAP	MF_00047. Dala_Dala_lig.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR016185. PreATP-grasp_dom. [Graphical view]
PANTHER	PTHR23132. PTHR23132. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C7T8V0_LACRG

Accession

Primary (citable) accession number: C7T8V0
Secondary accession number(s): C8UYQ5

Entry history

Integrated into UniProtKB/TrEMBL:	October 13, 2009
Last sequence update:	October 13, 2009
Last modified:	May 29, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information