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C7T2J9 (PORED_MESAU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 16. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Polyprenol reductase

EC=1.3.1.94
Alternative name(s):
3-oxo-5-alpha-steroid 4-dehydrogenase 3
EC=1.3.1.22
Steroid 5-alpha-reductase 3
Short name=S5AR 3
Short name=SR type 3
Gene names
Name:Srd5a3
OrganismMesocricetus auratus (Golden hamster)
Taxonomic identifier10036 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

Protein attributes

Sequence length330 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism. Also able to convert testosterone (T) into 5-alpha-dihydrotestosterone (DHT) By similarity.

Catalytic activity

Ditrans,polycis-dolichol + NADP+ = ditrans,polycis-polyprenol + NADPH.

A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the steroid 5-alpha reductase family. Polyprenol reductase subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 330330Polyprenol reductase
PRO_0000398649

Regions

Topological domain1 – 1919Cytoplasmic Potential
Transmembrane20 – 4021Helical; Potential
Topological domain41 – 7434Lumenal Potential
Transmembrane75 – 9521Helical; Potential
Topological domain96 – 13237Cytoplasmic Potential
Transmembrane133 – 15321Helical; Potential
Topological domain154 – 16815Lumenal Potential
Transmembrane169 – 18921Helical; Potential
Topological domain190 – 20617Cytoplasmic Potential
Transmembrane207 – 22721Helical; Potential
Topological domain228 – 27750Lumenal Potential
Transmembrane278 – 29821Helical; Potential
Topological domain299 – 33032Cytoplasmic Potential

Sequences

Sequence LengthMass (Da)Tools
C7T2J9 [UniParc].

Last modified October 13, 2009. Version 1.
Checksum: 6E7990C37A6D27D6

FASTA33038,164
        10         20         30         40         50         60 
MASWVGTELS ALNPLRTLWL ALAAAFLLAL LLQLAPAGLL PNCALFQDLI RYGKTKLSGP 

        70         80         90        100        110        120 
RRPAVCRAFD VPKRYFSHFY VVSVLWNGFL LWFLSRSLFL GAPFPNWLRA LLRTLGSTQF 

       130        140        150        160        170        180 
RALEMESKAS QMLVGELALS AFLVLVFLWV HSVRRLFECF YISVFSNAVM HVVQYCFGLV 

       190        200        210        220        230        240 
YYVLVGLTVL SQVPMDDKNV YMLGKNLLLP ARWFHVLGMM MFLWSSAHQY ECHVILSNLR 

       250        260        270        280        290        300 
RNKKGAIVHC QHRIPFGDWF EYVSSANYLA ELMIYISMAV TFGFHNFTWW LVVAYVFFCQ 

       310        320        330 
ALSAFFNHKF YKSTFVSYPK HRKAFLPFLF 

« Hide

References

[1]"Cloning, sequence analysis and tissue distribution of the steroid 5alpha-reductase 3 (Srd5a3) full-length cDNA from Syrian hamster."
Vilchis F., Ramos L., Chavez B.
Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Harderian gland.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FJ851160 mRNA. Translation: ACV30167.1.
RefSeqNP_001268635.1. NM_001281706.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID101843963.

Organism-specific databases

CTD79644.

Enzyme and pathway databases

UniPathwayUPA00378.

Family and domain databases

InterProIPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamPF02544. Steroid_dh. 1 hit.
[Graphical view]
PROSITEPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePORED_MESAU
AccessionPrimary (citable) accession number: C7T2J9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: October 13, 2009
Last modified: February 19, 2014
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways