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C7RMJ3 (C7RMJ3_ACCPU) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Glutathione synthetase HAMAP-Rule MF_00162

EC=6.3.2.3 HAMAP-Rule MF_00162
Alternative name(s):
GSH synthetase HAMAP-Rule MF_00162
Glutathione synthase HAMAP-Rule MF_00162
Gene names
Name:gshB HAMAP-Rule MF_00162
Ordered Locus Names:CAP2UW1_0674 EMBL ACV34020.1
OrganismAccumulibacter phosphatis (strain UW-1) [Complete proteome] [HAMAP] EMBL ACV34020.1
Taxonomic identifier522306 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaCandidatus Accumulibacter

Protein attributes

Sequence length318 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity. HAMAP-Rule MF_00162

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162

Sequence similarities

Belongs to the prokaryotic GSH synthase family. HAMAP-Rule MF_00162

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00162

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain125 – 310186ATP-grasp By similarity HAMAP-Rule MF_00162
Nucleotide binding151 – 20757ATP By similarity HAMAP-Rule MF_00162

Sites

Metal binding2811Magnesium or manganese By similarity HAMAP-Rule MF_00162
Metal binding2831Magnesium or manganese By similarity HAMAP-Rule MF_00162

Sequences

Sequence LengthMass (Da)Tools
C7RMJ3 [UniParc].

Last modified October 13, 2009. Version 1.
Checksum: C2A0E9B613190CB2

FASTA31834,747
        10         20         30         40         50         60 
MRIAFVVDPL GSLRAYKDSS VAMMRAAQAR GHAVWAVQQE AIHLSPLHGV CAEAVHLEML 

        70         80         90        100        110        120 
ADDHDWFVEI DRHVVALRDF GAVVMRKDPP FDIEYVTTTW LLERAEGEGA RVFNRPRALR 

       130        140        150        160        170        180 
DHSEKLSVAE FARFAVPTLV AREAALIHAF IDLERDTILK PLDGMGGSQI FRVRDDDANR 

       190        200        210        220        230        240 
NVIVETLTRD GARTIMAQRY IPEIVDGDKR ILLVGGQVVP WCLARIPKAG ETRGNLAAGG 

       250        260        270        280        290        300 
TGVARELTAR DHEIASALAP VLAARGLLLV GLDVIGDWLT EINVTSPTCM VEIAEQTGFD 

       310 
AAEMFVAALE RACAVAAV 

« Hide

References

[1]"Complete sequence of chromosome of Candidatus Accumulibacter phosphatis clade IIA str. UW-1."
US DOE Joint Genome Institute
Martin H.G., Ivanova N., Kunin V., Warnecke F., Barry K., He S., Salamov A., Szeto E., Dalin E., Pangilinan J.L., Lapidus A., Lowry S., Kyrpides N.C., McMahon K.D., Hugenholtz P.
Submitted (SEP-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: UW-1 EMBL ACV34020.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001715 Genomic DNA. Translation: ACV34020.1.
RefSeqYP_003165949.1. NC_013194.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING522306.CAP2UW1_0674.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACV34020; ACV34020; CAP2UW1_0674.
GeneID8402806.
KEGGapp:CAP2UW1_0674.
PATRIC21257356. VBICanAcc132554_0944.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0189.
HOGENOMHOG000265022.
KOK01920.
OrthoDBEOG6WMHWB.

Enzyme and pathway databases

BioCycAPHO522306:GHXL-680-MONOMER.
UniPathwayUPA00142; UER00210.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00162. GSH_S.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01380. glut_syn. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC7RMJ3_ACCPU
AccessionPrimary (citable) accession number: C7RMJ3
Entry history
Integrated into UniProtKB/TrEMBL: October 13, 2009
Last sequence update: October 13, 2009
Last modified: June 11, 2014
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)