Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutathione synthetase

Gene

gshB

Organism
Accumulibacter phosphatis (strain UW-1)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 1 magnesium or manganese ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi281 – 2811Magnesium or manganeseUniRule annotation
Metal bindingi283 – 2831Magnesium or manganeseUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi151 – 20757ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutathione synthase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. manganese ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Biological processi

Glutathione biosynthesisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciAPHO522306:GHXL-680-MONOMER.
UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Alternative name(s):
GSH synthetaseUniRule annotation
Glutathione synthaseUniRule annotation
Gene namesi
Name:gshBUniRule annotation
Ordered Locus Names:CAP2UW1_0674Imported
OrganismiAccumulibacter phosphatis (strain UW-1)Imported
Taxonomic identifieri522306 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaCandidatus Accumulibacter
ProteomesiUP000001619 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: InterPro
Complete GO annotation...

Interactioni

Protein-protein interaction databases

STRINGi522306.CAP2UW1_0674.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini125 – 310186ATP-graspUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic GSH synthase family.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0189.
HOGENOMiHOG000265022.
KOiK01920.
OrthoDBiEOG6WMHWB.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_00162. GSH_S.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01380. glut_syn. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C7RMJ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIAFVVDPL GSLRAYKDSS VAMMRAAQAR GHAVWAVQQE AIHLSPLHGV
60 70 80 90 100
CAEAVHLEML ADDHDWFVEI DRHVVALRDF GAVVMRKDPP FDIEYVTTTW
110 120 130 140 150
LLERAEGEGA RVFNRPRALR DHSEKLSVAE FARFAVPTLV AREAALIHAF
160 170 180 190 200
IDLERDTILK PLDGMGGSQI FRVRDDDANR NVIVETLTRD GARTIMAQRY
210 220 230 240 250
IPEIVDGDKR ILLVGGQVVP WCLARIPKAG ETRGNLAAGG TGVARELTAR
260 270 280 290 300
DHEIASALAP VLAARGLLLV GLDVIGDWLT EINVTSPTCM VEIAEQTGFD
310
AAEMFVAALE RACAVAAV
Length:318
Mass (Da):34,747
Last modified:October 13, 2009 - v1
Checksum:iC2A0E9B613190CB2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001715 Genomic DNA. Translation: ACV34020.1.
RefSeqiWP_015765268.1. NC_013194.1.
YP_003165949.1. NC_013194.1.

Genome annotation databases

EnsemblBacteriaiACV34020; ACV34020; CAP2UW1_0674.
KEGGiapp:CAP2UW1_0674.
PATRICi21257356. VBICanAcc132554_0944.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001715 Genomic DNA. Translation: ACV34020.1.
RefSeqiWP_015765268.1. NC_013194.1.
YP_003165949.1. NC_013194.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi522306.CAP2UW1_0674.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACV34020; ACV34020; CAP2UW1_0674.
KEGGiapp:CAP2UW1_0674.
PATRICi21257356. VBICanAcc132554_0944.

Phylogenomic databases

eggNOGiCOG0189.
HOGENOMiHOG000265022.
KOiK01920.
OrthoDBiEOG6WMHWB.

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210.
BioCyciAPHO522306:GHXL-680-MONOMER.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_00162. GSH_S.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01380. glut_syn. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome of Candidatus Accumulibacter phosphatis clade IIA str. UW-1."
    US DOE Joint Genome Institute
    Martin H.G., Ivanova N., Kunin V., Warnecke F., Barry K., He S., Salamov A., Szeto E., Dalin E., Pangilinan J.L., Lapidus A., Lowry S., Kyrpides N.C., McMahon K.D., Hugenholtz P.
    Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: UW-1Imported.

Entry informationi

Entry nameiC7RMJ3_ACCPU
AccessioniPrimary (citable) accession number: C7RMJ3
Entry historyi
Integrated into UniProtKB/TrEMBL: October 13, 2009
Last sequence update: October 13, 2009
Last modified: April 1, 2015
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.