Skip Header

Contribute Send feedback
Read comments (?) or add your own

C7R1J9 (C7R1J9_JONDD) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 20. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry infoCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518
Gene names
Ordered Locus Names:Jden_2197
OrganismJonesia denitrificans (strain ATCC 14870 / DSM 20603 / CIP 55134) (Listeria denitrificans) [Complete proteome] [HAMAP]
Taxonomic identifier471856 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Jonesiaceae › Jonesia

Protein attributes

Sequence length923 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733

Sequence similarities

Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206

Contains 1 Toprim domain. SAAS SAAS023406

Sequences

Sequence LengthMass (Da)Tools
C7R1J9 [UniParc].

Last modified October 13, 2009. Version 1.
Checksum: 736B461BB626AF6F

FASTA923101,044
        10         20         30         40         50         60 
MSQGRKLVIV ESPAKARTIS AYLGADFDVE ASVGHIRDLP QPSELPAEMK KSGYGKFAVD 

        70         80         90        100        110        120 
VDNGFAPYYE VYPDKKKKVT ELRRLLKDAD ELYLATDEDR EGEAIAWHLL EALKPKVPVK 

       130        140        150        160        170        180 
RMVFHEITRE AIQRALTTTR DLDERLVDAQ ETRRILDRLY GYEVSPVLWR KVRQGLSAGR 

       190        200        210        220        230        240 
VQSVATRLVV ERERERMAFV PASYWDVTAQ FEATQGQDAG GTFQARLTHV DGTRVATGRD 

       250        260        270        280        290        300 
FTDRGELKAR DVVHLTEDAA TTLAQSLTDA PATVASLDTK PYTRRPAAPF TTSTLQQEAS 

       310        320        330        340        350        360 
RKLRMSSKTA MRVAQTLYEN GYITYMRTDS PALSAQAISA ARSQAADLYG TEFVPEKPRH 

       370        380        390        400        410        420 
YASKSKGAQE AHEAIRPAGD TFRTPAQVAN VLSGDQFRLY ELIWKRTVAS QMADARGSTA 

       430        440        450        460        470        480 
SVRIDVPGQV DGQTTTATFS ASGTVITFRG FLAAYEEGRD VDRYDDGSQS DGRLPHMVEG 

       490        500        510        520        530        540 
DPLASRDVSA DGHTTTPPPR FTEASLVKAL EERGIGRPST YASTISTIQD RGYVNNRGQA 

       550        560        570        580        590        600 
LVPSWLAFSV IRLLEEHFSQ LIDYDFTAQM ESDLDEIAAG QGDRVNWLTQ FYFGGAGGDK 

       610        620        630        640        650        660 
AGLKELVEGL GDIDAREINT VSLGEGIVLR VGRYGPYIEH LTADTAEGEN PPRASVPDDI 

       670        680        690        700        710        720 
APDELTLDVA RDLLENQKDG DIELGSDPVS GHMIVAKSGR FGPYVTEIIP EPELDESMSA 

       730        740        750        760        770        780 
AAKKKAKAAL PKPRTASLFK SHSLTTITLD EALQLLSLPR VVGVDPDTGT EITANNGRYG 

       790        800        810        820        830        840 
PYLKKGTDSR TITSEEQLLT ITLDEALEIY KQPKRGRGAT ASAPLRELGV DPTSEKPIVI 

       850        860        870        880        890        900 
KDGRFGPYVT DGKTNRTLPK DATVESVTFE QAVELLAEKR AKAPVKRATR TTAKKSTATK 

       910        920 
KTTTTAAKKT PAKKTTATKK TQS

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001706 Genomic DNA. Translation: ACV09834.1.
RefSeqYP_003162137.1. NC_013174.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGC7R1J9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID8381355.
GenomeReviewsGene locus Jden_2197 in contig CP001706_GR.
KEGGjde:Jden_2197.
PATRIC22164643. VBIJonDen9837_2288.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAKKVMPRL.
ProtClustDBCLSK2488184.

Family and domain databases

InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR006171. Toprim_domain.
[Graphical view]
Gene3DG3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits.
G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KOK03168.
PANTHERPTHR11390. Topo_IA. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. Topo_IA_core. 1 hit.
TIGRFAMsTIGR01051. TopA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC7R1J9_JONDD
AccessionPrimary (citable) accession number: C7R1J9
Entry history
Integrated into UniProtKB/TrEMBL: October 13, 2009
Last sequence update: October 13, 2009
Last modified: December 14, 2011
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry info