C7PKX5 (C7PKX5_CHIPD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172
EC=2.1.1.14 HAMAP MF_00172

Alternative name(s):
Cobalamin-independent methionine synthase HAMAP MF_00172
Methionine synthase, vitamin-B12 independent isozyme HAMAP MF_00172

Gene names

Name:	metE HAMAP MF_00172
Ordered Locus Names:	Cpin_6481

Organism

Chitinophaga pinensis (strain ATCC 43595 / DSM 2588 / NCIB 11800 / UQM 2034) [Complete proteome] [HAMAP]

Taxonomic identifier

485918 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Sphingobacteriia › Sphingobacteriales › Chitinophagaceae › Chitinophaga

Protein attributes

Sequence length	769 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172 SAAS SAAS013215
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172 SAAS SAAS013215
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172 SAAS SAAS013215
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family. HAMAP MF_00172

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis HAMAP MF_00172 SAAS SAAS013215
Domain	Repeat HAMAP MF_00172
Ligand	Metal-binding HAMAP MF_00172 SAAS SAAS013215 Zinc HAMAP MF_00172 SAAS SAAS013215
Molecular function	Methyltransferase HAMAP MF_00172 SAAS SAAS013215 EMBL ACU63885.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	652	1	Zinc By similarity HAMAP MF_00172
	Metal binding	654	1	Zinc By similarity HAMAP MF_00172
	Metal binding	737	1	Zinc By similarity HAMAP MF_00172

Sequences

	Sequence		Length	Mass (Da)
	C7PKX5 [UniParc]. Last modified October 13, 2009. Version 1. Checksum: 5C16B17A6FB55B07	FASTA	769	86,662
	10 20 30 40 50 60 MQTNILGYPR IGSNRELKKA NEAYWTGKIS LEKLQLTARQ LRRQNWETLH QAGIDLIPSN 70 80 90 100 110 120 DFSYYDQILD MCIMTGAIPA RFQALKESLV TPSSPELYFA MARGYQKNGF DATAMEMTKW 130 140 150 160 170 180 FDTNYHYLVP EFDASQEFSL LYNKCLEEFK EAQAIGIHTK PVLVGPVSFL LLGKIKEESL 190 200 210 220 230 240 QLPALLDKLL PVYIQVLQSL YQAGATWIQI DEPTLVLDLT PAQQDLFLQA YAKIRKAVPA 250 260 270 280 290 300 LKLLLTTYFG GLQDNTALTT QLPVDALHID LVRAPEQLDT LLAALPAGLQ LSVGVVNGRN 310 320 330 340 350 360 IWKNNYDTSL LLINKAIAAL GRERVLIATS CSLLHTPYDL ELETDESVLT PQIKNWMAFA 370 380 390 400 410 420 KQKVYEVIAL KDILNGHDEL LLQTNQHTMQ ERATAAIIHK PAVKARLAAL TPADAARKSI 430 440 450 460 470 480 FTARQKAQEE VLQLPLFPTT TIGSFPQTED IRKLRADLKK GDITKEGYDT AIRKAIQDSV 490 500 510 520 530 540 QLQESLRIDV LVHGEFERND MVEYFGEQLE GFVFTKNGWV QSYGSRCVKP PVIYGDVQRS 550 560 570 580 590 600 TPMTVEWSSY AQSLTNKPMK GMLTGPVTIL QWSFVRDDQP RAETTLQIAL AIRDEVVDLE 610 620 630 640 650 660 QAGIRVIQVD EPAIREGLPL RQADWQSYLD WAVKAFRVSV SGVEDATQIH THMCYSEFND 670 680 690 700 710 720 IISNIAAMDA DVITIETSRS QMELLEVFST FHYPYEIGPG VYDIHSPRVP TVAEMTELLE 730 740 750 760 KAIQLLPARN IWVNPDCGLK TRKWPETEQA LRNMVAAARN MRVAVQTMV

References

[1]

"The complete genome of Chitinophaga pinensis DSM 2588." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kyrpides N., Mavromatis K., Ivanova N., Mikhailova N., Sims D., Meinche L., Brettin T., Detter J.C., Han C. , Larimer F., Land M., Hauser L., Markowitz V., Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Spring S., Klenk H.-P., Eisen J.A. Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43595 / DSM 2588 / NCIB 11800 / UQM 2034.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001699 Genomic DNA. Translation: ACU63885.1.
RefSeq	YP_003126086.1. NC_013132.1.
3D structure databases
ProteinModelPortal	C7PKX5.
ModBase	Search...
Protein-protein interaction databases
STRING	C7PKX5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	8362665.
GenomeReviews	Gene locus Cpin_6481 in contig CP001699_GR.
KEGG	cpi:Cpin_6481.
PATRIC	21361540. VBIChiPin99107_6380.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	RNIWRAN.
ProtClustDB	PRK05222.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

C7PKX5_CHIPD

Accession

Primary (citable) accession number: C7PKX5

Entry history

Integrated into UniProtKB/TrEMBL:	October 13, 2009
Last sequence update:	October 13, 2009
Last modified:	December 14, 2011
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)