C7P996 (C7P996_METFA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA polymerase II large subunit HAMAP MF_00324
Short name=Pol II HAMAP MF_00324
EC=2.7.7.7 HAMAP MF_00324

Gene names

Name:	polC HAMAP MF_00324
Ordered Locus Names:	Mefer_1319

Organism

Methanocaldococcus fervens (strain DSM 4213 / JCM 157852 / AG86) (Methanococcus fervens) [Complete proteome] [HAMAP]

Taxonomic identifier

573064 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	1137 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity. HAMAP MF_00324
Catalytic activity	Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP MF_00324 Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00324
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity. HAMAP MF_00324
Sequence similarities	Belongs to the archaeal DNA polymerase II family. HAMAP MF_00324

Ontologies

Keywords
Biological process	DNA replication HAMAP MF_00324
Ligand	DNA-binding HAMAP MF_00324
Molecular function	DNA-directed DNA polymerase HAMAP MF_00324 Exonuclease HAMAP MF_00324 Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme HAMAP MF_00324
Gene Ontology (GO)
Biological process	DNA catabolic process, exonucleolytic Inferred from electronic annotation. Source: HAMAP DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	C7P996 [UniParc]. Last modified October 13, 2009. Version 1. Checksum: 7913F3D49622A829	FASTA	1,137	129,011
	10 20 30 40 50 60 MVHVACSENM KKYFESIVDE VKKIYKIAEE CRKKGFDPVD EVEIPLAADM ADRVEGLVGP 70 80 90 100 110 120 KGVAGRIREL VKELGKEPAA LEIAKEIVEG KFGDFDKEKK AEQAVRTALA VLTEGIVAAP 130 140 150 160 170 180 LEGIADVKIK KNPDGSEYLA IYYAGPIRSA GGTAQALSVL VGDFVRKAMG LDRYKPTEDE 190 200 210 220 230 240 IERYVEEVEL YQSEVGSFQY NPKADEIRTA VRNIPIEITG EATDDVEVSG HRDLPRVETN 250 260 270 280 290 300 QLRGGALLVL VEGVLLKAPK ILRHVDKLGI EGWDWLKDLM SKKEEEEEEK DEKIDDEDVD 310 320 330 340 350 360 EEEEEISGYW RDIKIEANKK FISEVIAGRP VFAHPSKVGG FRLRYGRSRN TGFATQGFHP 370 380 390 400 410 420 ALMYLVDEFM AVGTQLKTER PGKATCVVPV DSIEPPIVKL KNGDVIRVDT IEKAIEVRDR 430 440 450 460 470 480 VEEILFLGDV LVNYGDFLEN NHPLLPSCWC EEWYEKVLIA SNVGYDKEFI KNPKPEEAVK 490 500 510 520 530 540 FALETKTPLH PRYTYHWHDV SKEDIILLRN WLLKGKEDDF EGKKVWIVGL ETEDDKKAKR 550 560 570 580 590 600 ILELIGCCHL VRNKKVMIEE YYPLLYSLGY DVENKKDLIE NIDEILNSAK NSMHLINLLA 610 620 630 640 650 660 PFEVRRNTYV YVGARMGRPE KAAPRKMKPP VNGLFPIGNA GGQVRLINKA VEENNTDDID 670 680 690 700 710 720 VSYTRCPKCG KISLYRVCPF CGTKVELDTF GKIKAPLKEY WYAALKRLGI NKPGDVKCIK 730 740 750 760 770 780 GMTSKQKIVE PLEKAILRAM NEVYVFKDGT TRFDCTDVPV THFKPNEINV SVEKLRELGY 790 800 810 820 830 840 DKDIYGNELV DGDQVVELKP QDVIIPESCA EYFVKVANFI DDLLEKFYNM ERFYNVKKKE 850 860 870 880 890 900 DLIGHLVIGM APHTSAGMVG RIIGYTKANV GYAHPYFHAA KRRNCDGDED SFFLLLDAFL 910 920 930 940 950 960 NFSKKFLPDK RGGQMDAPLV LTTILDPKEV DGEVHNMDTM WSYPLEFYEK TLEMPSPKEV 970 980 990 1000 1010 1020 KEFMETVEDR LGNPEQYEGI GYTHETSRID LGPKVCAYKT LGSMLEKTTS QLSVAKKIRA 1030 1040 1050 1060 1070 1080 TDERDVAEKV IQSHFIPDLI GNLRAFSRQA VRCKCGAKFR RIPLRGKCPK CGSNLILTVS 1090 1100 1110 1120 1130 KGAVEKYMDV AEKMAEEYDV NNYIKQRLKI IREGINSIFE NDKSKQVKLS DFFKMKT

References

[1]

"Complete sequence of chromosome of Methanocaldococcus fervens AG86." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Detter J.C., Han C., Tapia R., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Lupa-Sieprawska M., Whitman W.B. Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 4213 / JCM 157852 / AG86.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001696 Genomic DNA. Translation: ACV25128.1.
RefSeq	YP_003128628.1. NC_013156.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	C7P996.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	8366022.
GenomeReviews	Gene locus Mefer_1319 in contig CP001696_GR.
KEGG	mfe:Mefer_1319.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	NCDGDED.
ProtClustDB	PRK04023.
Family and domain databases
HAMAP	MF_00324. DNApol_II_L_arch. [Tree]
InterPro	IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view]
KO	K02322.
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
PIRSF	PIRSF016275. PolC_DP2. 1 hit.
TIGRFAMs	TIGR00354. PolC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

C7P996_METFA

Accession

Primary (citable) accession number: C7P996

Entry history

Integrated into UniProtKB/TrEMBL:	October 13, 2009
Last sequence update:	October 13, 2009
Last modified:	November 16, 2011
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)