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C7P995

- C7P995_METFA

UniProt

C7P995 - C7P995_METFA

Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Methanocaldococcus fervens (strain DSM 4213 / JCM 157852 / AG86) (Methanococcus fervens)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 37 (01 Oct 2014)
      Sequence version 1 (13 Oct 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

    Catalytic activityi

    2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
    3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei153 – 1531Proton acceptorUniRule annotation
    Binding sitei155 – 1551SubstrateUniRule annotation
    Metal bindingi179 – 1791Magnesium; via carbamate groupUniRule annotation
    Metal bindingi181 – 1811MagnesiumUniRule annotation
    Metal bindingi182 – 1821MagnesiumUniRule annotation
    Active sitei269 – 2691Proton acceptorUniRule annotation
    Binding sitei270 – 2701SubstrateUniRule annotation
    Binding sitei302 – 3021SubstrateUniRule annotation
    Sitei309 – 3091Transition state stabilizerUniRule annotation

    GO - Molecular functioni

    1. magnesium ion binding Source: UniProtKB-HAMAP
    2. oxidoreductase activity Source: UniProtKB-KW
    3. ribulose-bisphosphate carboxylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. AMP catabolic process Source: UniProtKB-HAMAP
    2. carbon fixation Source: UniProtKB-KW

    Keywords - Molecular functioni

    LyaseUniRule annotationImported, OxidoreductaseUniRule annotation

    Keywords - Biological processi

    Carbon dioxide fixationUniRule annotation

    Keywords - Ligandi

    MagnesiumUniRule annotation, Metal-bindingUniRule annotation

    Enzyme and pathway databases

    BioCyciMFER573064:GHUI-1360-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
    Short name:
    RuBisCOUniRule annotation
    Gene namesi
    Name:rbcLUniRule annotation
    Ordered Locus Names:Mefer_1318Imported
    OrganismiMethanocaldococcus fervens (strain DSM 4213 / JCM 157852 / AG86) (Methanococcus fervens)Imported
    Taxonomic identifieri573064 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
    ProteomesiUP000001495: Chromosome

    PTM / Processingi

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei179 – 1791N6-carboxylysineUniRule annotation

    Interactioni

    Subunit structurei

    Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

    Protein-protein interaction databases

    STRINGi573064.Mefer_1318.

    Structurei

    3D structure databases

    ProteinModelPortaliC7P995.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni353 – 3553Substrate bindingUniRule annotation
    Regioni375 – 3784Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1850.
    HOGENOMiHOG000230831.
    KOiK01601.
    OMAiFTQDWAS.

    Family and domain databases

    Gene3Di3.20.20.110. 1 hit.
    3.30.70.150. 1 hit.
    HAMAPiMF_01133. RuBisCO_L_type3.
    InterProiIPR017712. RuBisCO_III.
    IPR000685. RuBisCO_lsu_C.
    IPR017443. RuBisCO_lsu_fd_N.
    IPR017444. RuBisCO_lsu_N.
    [Graphical view]
    PfamiPF00016. RuBisCO_large. 1 hit.
    PF02788. RuBisCO_large_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51649. SSF51649. 1 hit.
    SSF54966. SSF54966. 1 hit.
    TIGRFAMsiTIGR03326. rubisco_III. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    C7P995-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYIDLNYTP NENDLLSCMI IKGENLEKLA NEIAGESSIG TWTKVQTMKS    50
    DIYTKLKPKV YEIKEIGNEG RYKIGLIKIA YPLYAFEINN MPGVLAGIAG 100
    NIFGMKIAKG LRILDFRFPE EFVKSYKGPR FGIEGVRKTL KIKERPLLGT 150
    IVKPKVGLRT EEHAKVAYEA WVGGVDLVKD DENLTSQEFN KFEDRVYKTL 200
    EMRDKAEEET GERKAYMPNI TAPYREMLRR AEIAEDAGSE YVMIDVVVSG 250
    FSAVQSFREE EFNFIIHAHR AMHAAITRGR DFGISMLALA KIYRLLGVDQ 300
    LHIGTVVGKM EGGEKEVKAI RDEIVYDKVE ADNENKFFNQ EWFGINPIFP 350
    VSSGGVHPKL VPKIVEILGR DLIIQAGGGV HGHPDGTKAG AKAMRAAIEA 400
    VVEGKSLEEK AEEVEELKKA LGYWK 425
    Length:425
    Mass (Da):47,698
    Last modified:October 13, 2009 - v1
    Checksum:i0CC57F4A783A5602
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001696 Genomic DNA. Translation: ACV25127.1.
    RefSeqiYP_003128627.1. NC_013156.1.

    Genome annotation databases

    EnsemblBacteriaiACV25127; ACV25127; Mefer_1318.
    GeneIDi8366021.
    KEGGimfe:Mefer_1318.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001696 Genomic DNA. Translation: ACV25127.1 .
    RefSeqi YP_003128627.1. NC_013156.1.

    3D structure databases

    ProteinModelPortali C7P995.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 573064.Mefer_1318.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACV25127 ; ACV25127 ; Mefer_1318 .
    GeneIDi 8366021.
    KEGGi mfe:Mefer_1318.

    Phylogenomic databases

    eggNOGi COG1850.
    HOGENOMi HOG000230831.
    KOi K01601.
    OMAi FTQDWAS.

    Enzyme and pathway databases

    BioCyci MFER573064:GHUI-1360-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.110. 1 hit.
    3.30.70.150. 1 hit.
    HAMAPi MF_01133. RuBisCO_L_type3.
    InterProi IPR017712. RuBisCO_III.
    IPR000685. RuBisCO_lsu_C.
    IPR017443. RuBisCO_lsu_fd_N.
    IPR017444. RuBisCO_lsu_N.
    [Graphical view ]
    Pfami PF00016. RuBisCO_large. 1 hit.
    PF02788. RuBisCO_large_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51649. SSF51649. 1 hit.
    SSF54966. SSF54966. 1 hit.
    TIGRFAMsi TIGR03326. rubisco_III. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of chromosome of Methanocaldococcus fervens AG86."
      US DOE Joint Genome Institute
      Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Detter J.C., Han C., Tapia R., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Lupa-Sieprawska M., Whitman W.B.
      Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 4213 / JCM 157852 / AG86Imported.

    Entry informationi

    Entry nameiC7P995_METFA
    AccessioniPrimary (citable) accession number: C7P995
    Entry historyi
    Integrated into UniProtKB/TrEMBL: October 13, 2009
    Last sequence update: October 13, 2009
    Last modified: October 1, 2014
    This is version 37 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Miscellaneous

    Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3