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Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Methanocaldococcus fervens (strain DSM 4213 / JCM 15782 / AG86) (Methanococcus fervens)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei153Proton acceptorUniRule annotation1
Binding sitei155SubstrateUniRule annotation1
Metal bindingi179Magnesium; via carbamate groupUniRule annotation1
Metal bindingi181MagnesiumUniRule annotation1
Metal bindingi182MagnesiumUniRule annotation1
Active sitei269Proton acceptorUniRule annotation1
Binding sitei270SubstrateUniRule annotation1
Binding sitei302SubstrateUniRule annotation1
Sitei309Transition state stabilizerUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationImported, OxidoreductaseUniRule annotation

Keywords - Biological processi

Carbon dioxide fixationUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCOUniRule annotation
Gene namesi
Name:rbcLUniRule annotation
Ordered Locus Names:Mefer_1318Imported
OrganismiMethanocaldococcus fervens (strain DSM 4213 / JCM 15782 / AG86) (Methanococcus fervens)Imported
Taxonomic identifieri573064 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000001495 Componenti: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei179N6-carboxylysineUniRule annotation1

Interactioni

Subunit structurei

Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

Protein-protein interaction databases

STRINGi573064.Mefer_1318.

Structurei

3D structure databases

ProteinModelPortaliC7P995.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 122RuBisCO_large_NInterPro annotationAdd BLAST121
Domaini132 – 424RuBisCO_largeInterPro annotationAdd BLAST293

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni353 – 355Substrate bindingUniRule annotation3
Regioni375 – 378Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04443. Archaea.
COG1850. LUCA.
HOGENOMiHOG000230831.
KOiK01601.
OMAiFTQDWAS.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01133. RuBisCO_L_type3. 1 hit.
InterProiIPR033966. RuBisCO.
IPR017712. RuBisCO_III.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsiTIGR03326. rubisco_III. 1 hit.

Sequencei

Sequence statusi: Complete.

C7P995-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYIDLNYTP NENDLLSCMI IKGENLEKLA NEIAGESSIG TWTKVQTMKS
60 70 80 90 100
DIYTKLKPKV YEIKEIGNEG RYKIGLIKIA YPLYAFEINN MPGVLAGIAG
110 120 130 140 150
NIFGMKIAKG LRILDFRFPE EFVKSYKGPR FGIEGVRKTL KIKERPLLGT
160 170 180 190 200
IVKPKVGLRT EEHAKVAYEA WVGGVDLVKD DENLTSQEFN KFEDRVYKTL
210 220 230 240 250
EMRDKAEEET GERKAYMPNI TAPYREMLRR AEIAEDAGSE YVMIDVVVSG
260 270 280 290 300
FSAVQSFREE EFNFIIHAHR AMHAAITRGR DFGISMLALA KIYRLLGVDQ
310 320 330 340 350
LHIGTVVGKM EGGEKEVKAI RDEIVYDKVE ADNENKFFNQ EWFGINPIFP
360 370 380 390 400
VSSGGVHPKL VPKIVEILGR DLIIQAGGGV HGHPDGTKAG AKAMRAAIEA
410 420
VVEGKSLEEK AEEVEELKKA LGYWK
Length:425
Mass (Da):47,698
Last modified:October 13, 2009 - v1
Checksum:i0CC57F4A783A5602
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001696 Genomic DNA. Translation: ACV25127.1.
RefSeqiWP_015791861.1. NC_013156.1.

Genome annotation databases

EnsemblBacteriaiACV25127; ACV25127; Mefer_1318.
GeneIDi8366021.
KEGGimfe:Mefer_1318.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001696 Genomic DNA. Translation: ACV25127.1.
RefSeqiWP_015791861.1. NC_013156.1.

3D structure databases

ProteinModelPortaliC7P995.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi573064.Mefer_1318.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACV25127; ACV25127; Mefer_1318.
GeneIDi8366021.
KEGGimfe:Mefer_1318.

Phylogenomic databases

eggNOGiarCOG04443. Archaea.
COG1850. LUCA.
HOGENOMiHOG000230831.
KOiK01601.
OMAiFTQDWAS.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01133. RuBisCO_L_type3. 1 hit.
InterProiIPR033966. RuBisCO.
IPR017712. RuBisCO_III.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsiTIGR03326. rubisco_III. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiC7P995_METFA
AccessioniPrimary (citable) accession number: C7P995
Entry historyi
Integrated into UniProtKB/TrEMBL: October 13, 2009
Last sequence update: October 13, 2009
Last modified: November 30, 2016
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

Keywords - Technical termi

Complete proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.