C7P924 (C7P924_METFA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
RNA 3'-terminal phosphate cyclase HAMAP-Rule MF_00200
Short name=RNA cyclase HAMAP-Rule MF_00200
Short name=RNA-3'-phosphate cyclase HAMAP-Rule MF_00200
EC=6.5.1.4 HAMAP-Rule MF_00200

Gene names

Name:	rtcA HAMAP-Rule MF_00200
Ordered Locus Names:	Mefer_1247 EMBL ACV25056.1

Organism

Methanocaldococcus fervens (strain DSM 4213 / JCM 157852 / AG86) (Methanococcus fervens) [Complete proteome] [HAMAP] EMBL ACV25056.1

Taxonomic identifier

573064 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus › Methanocaldococcus fervens

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity. HAMAP-Rule MF_00200
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP-Rule MF_00200
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00200.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily. HAMAP-Rule MF_00200

Ontologies

Keywords
Cellular component	Cytoplasm HAMAP-Rule MF_00200
Ligand	ATP-binding HAMAP-Rule MF_00200 Nucleotide-binding
Molecular function	Ligase HAMAP-Rule MF_00200 EMBL ACV25056.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	280 – 284	5	ATP By similarity HAMAP-Rule MF_00200
Sites
	Active site	304	1	Tele-AMP-histidine intermediate By similarity HAMAP-Rule MF_00200
	Binding site	102	1	ATP By similarity HAMAP-Rule MF_00200

Sequences

	Sequence		Length	Mass (Da)
	C7P924 [UniParc]. Last modified October 13, 2009. Version 1. Checksum: 5ABDB0B55959A276	FASTA	338	37,222
	10 20 30 40 50 60 MDFIIIDGSY LEGGGQIVRT AVSLSALTQK PIKIINIRKK RKNRGLAHQH VSAVKAVKKL 70 80 90 100 110 120 CNAEVYGLKV GSEELTFIPS KLSPKDFTID IGTAGSISLV IQALLPLSLG IDKKFTVKIK 130 140 150 160 170 180 GGTDVKRAPP IDYIKNVTLK ILRDFGVLTE LKVLKRGFYP EGGGEVIFEV KPSKVKKFDL 190 200 210 220 230 240 IEHSKSDLVG GISYVQNLDE NIARRMRKRA VNLLNKRGLM PNIKIECSKG ISTGAGIVLW 250 260 270 280 290 300 NDTLGGSCLG EKGLRAEIVA ERAVDELLNA RKSGMALDKY MGDQIIPFLA FGKGVVGVSE 310 320 330 ITNHTKTNIW VVKHFLDVDF EIREYRENNC NGFTIEVV

References

[1]

"Complete sequence of chromosome of Methanocaldococcus fervens AG86." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Detter J.C., Han C., Tapia R., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Lupa-Sieprawska M., Whitman W.B. Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 4213 / JCM 157852 / AG86.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001696 Genomic DNA. Translation: ACV25056.1.
RefSeq	YP_003128556.1. NC_013156.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	573064.Mefer_1247.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACV25056; ACV25056; Mefer_1247.
GeneID	8365950.
KEGG	mfe:Mefer_1247.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0430.
HOGENOM	HOG000015264.
KO	K01974.
OMA	RRGHYPK.
ProtClustDB	PRK04204.
Enzyme and pathway databases
BioCyc	MFER573064:GHUI-1323-MONOMER.
Family and domain databases
Gene3D	3.30.360.20. 1 hit. 3.65.10.20. 2 hits.
HAMAP	MF_00200. RTC.
InterPro	IPR013791. RNA3'-term_phos_cycl_insert. IPR023797. RNA3'_phos_cyclase_dom. IPR000228. RNA3'_term_phos_cyc. IPR017770. RNA3'_term_phos_cyc_type_1. IPR020719. RNA3'_term_phos_cycl-like_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
PANTHER	PTHR11096. PTHR11096. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C7P924_METFA

Accession

Primary (citable) accession number: C7P924

Entry history

Integrated into UniProtKB/TrEMBL:	October 13, 2009
Last sequence update:	October 13, 2009
Last modified:	May 1, 2013
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)