C7P7F1 (C7P7F1_METFA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase HAMAP MF_00193 RuleBase RU003812
EC=6.3.1.5 HAMAP MF_00193 RuleBase RU003812

Gene names

Name:	nadE HAMAP MF_00193
Ordered Locus Names:	Mefer_0664

Organism

Methanocaldococcus fervens (strain DSM 4213 / JCM 157852 / AG86) (Methanococcus fervens) [Complete proteome] [HAMAP]

Taxonomic identifier

573064 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193 RuleBase RU003812
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. RuleBase RU004252 HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family. HAMAP MF_00193 RuleBase RU003811

Ontologies

Keywords
Ligand	ATP-binding HAMAP MF_00193 RuleBase RU003811 NAD HAMAP MF_00193 RuleBase RU003811 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00193 RuleBase RU003811 EMBL ACV24483.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	27 – 34	8	ATP By similarity HAMAP MF_00193
Sites
	Active site	29	1	By similarity HAMAP MF_00193

Sequences

	Sequence		Length	Mass (Da)
	C7P7F1 [UniParc]. Last modified October 13, 2009. Version 1. Checksum: B9D81CD3DAE9ED2D	FASTA	253	28,237
	10 20 30 40 50 60 MEKVIEKITN FIKEKVEEAK ANGVVIGLSG GIDSAVTAYL CVKALGKYRV LGIIMPEKNT 70 80 90 100 110 120 NPKDVEHAKM VAENLGIKYT ISDITDILKA FGAGGYVPTR EFDKIADGNL KARIRMCILY 130 140 150 160 170 180 YFANKYNLLV AGTSNKSEIY VGYGTKYGDT ACDIRPIGNL FKTEVRELAK YLGVPKEIIN 190 200 210 220 230 240 KPPSAGLWEG QTDEEELGIK YETLDKILKL YEEGKSKEEI SKELNISVDV VEHVFNLIKK 250 NEHKRTLPPT PEI

References

[1]

"Complete sequence of chromosome of Methanocaldococcus fervens AG86." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Detter J.C., Han C., Tapia R., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Lupa-Sieprawska M., Whitman W.B. Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 4213 / JCM 157852 / AG86.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001696 Genomic DNA. Translation: ACV24483.1.
RefSeq	YP_003127983.1. NC_013156.1.
3D structure databases
ProteinModelPortal	C7P7F1.
ModBase	Search...
Protein-protein interaction databases
STRING	C7P7F1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	8365339.
GenomeReviews	Gene locus Mefer_0664 in contig CP001696_GR.
KEGG	mfe:Mefer_0664.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	DGAVDCH.
ProtClustDB	CLSK2749980.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

C7P7F1_METFA

Accession

Primary (citable) accession number: C7P7F1

Entry history

Integrated into UniProtKB/TrEMBL:	October 13, 2009
Last sequence update:	October 13, 2009
Last modified:	December 14, 2011
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)